FastGene Dye Terminator Removal Kit
Dye Terminator Removal Kit
- cost efficient
- long shelf life - stocking at room temperature
FastGene® Dye Terminator Removal Kit
removes dye terminator molecules from sequencing samples using an efficient and reasonably priced gel filtration. The Kit includes a bottle of gelfiltration matrix, resuspension buffer and filter spin columns. At first an aliquot of the matrix will be mixed with resuspension buffer. Thereafter the equilibrated matrix is transferred into filter spin columns. After a brief spin up to 20 µl of the sequencing reaction can be loaded onto the column. The last centrifugation step will elute the purified sample. All kits can be stored at room temperature.
Specifications
| Parameter | Dye Terminator Removal |
| Max. sample volume | 20 µl sequencing reaction |
| Recovery | > 90% |
| Prep time | 5 minutes |
| Store at | room temperature |
Application data
 | Fig. 1: 20 µl sequencing reaction including 2,5 µl BigDye™ Terminator v3.1 was purified with FastGene® Dye Terminator Removal Kit and analyzed on an ABI 3100 sequencer. The DNA purified by the FastGene® Dye Terminator Removal Kit shows a very good performance in sequencing experiments, no dye blobs occur. |
Ordering information
| Product | Cat.No.: | Content |
| FastGene Dye Terminator Removal Kit | FG-9411 | 50 preps |
