FastGene Gel/PCR Extraction Kit
Gel PCR Extraction Kit
- 2 applications in 1 Kit - no extra buffer necessary
- Agarose Gel Band Cutter (5) free of charge in every kit
- 50 µl (for 10 agarose gels) Midori Green Advance free of charge in every kit
FastGene® Gel/PCR Extraction Kits
are designed for the extraction of DNA from agarose gels and for the purification of PCR products.
DNA fragments purified with FastGene® Gel/PCR Extraction Kits are ready for direct use in all common downstream applications, like sequencing, ligation and transformation, restriction digestion, microarray analysis, PCR and in vitro transcription.
Specifications
| Parameter | Gel Extraktion | PCR Clean-up |
| Max. sample volume | 300 mg Agarosegel | 100 µl PCR Mix |
| Gel | < 2,5% TAE oder TBE | |
| Typical recovery | 70-80% | 80-90% |
| Binding capacity | 10 µg | 10 µg |
| DNA fragment lenght | 50 bp - 10 kb | 50 bp - 10 kb |
| Primer removal | < 25 bp | |
| Elution volume | 20 - 50 µl | 20 -50 µl |
| Prep time | 20 minutes | 20 minutes |
| Format | spin column | spin column |
Application data
 | Fig. 1: PCR fragments of 300 bp were purified from 40 µl of a PCR stock solution using FastGene® Gel/PCR Extraction Kit and a competitor kit, according to manufacturers protocol. DNA was eluted in 20 µl elution buffer and 5 µl of eluate was analyzed on a 1,5% TAE agarose gel. The figure demonstrates that the FastGene® Gel/PCR Extraction Kit shows up to 90% of DNA recovery. |
| Fig. 2: 10 µl of a PCR stock solution with a 100 bp amplicon were applied on a TBE agarose gel. After electrophoresis the DNA was purified using a competitor kit and the FastGene® Gel/PCR Extraction Kit, according to manufacturers protocol. DNA was eluted in 20 µl elution buffer and 10 µl of eluate was analyzed on a 2% TAE agarose gel. The DNA recovery from agarose gels using FastGene® Gel/PCR Extraction Kits is very high even for small fragments. Lane 1: Unpurified PCR fragment; M = Marker Lane 2,3 and 4: Supplier M Lane 5,6 and 7: FastGene® Gel/PCR Extraction Kit |  |
Extra benefits
๏ In each kit 5 Agarose Gel Band Cutter and 50 µl Midori Green Advance DNA stain (for approximately 10 agarose gels) is included
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Ordering information
| Product | Cat.No.: | Content |
| FastGene Gel/PCR Extraction Kit | FG-91202 | 100 preps |
| FastGene Gel/PCR Extraction Kit | FG-91302 | 300 preps |
| FastGene Agarose Gel Band Cutter | FG-803 | 50 pieces |
