FastGene Plasmid Mini Kit
Plasmid Mini Kit
- High and low copy-plasmid DNA preparation kit
- Standard and Fast protocol
- LB tablets are included!!!
FastGene® Plasmid Mini Kits
are designed for rapid small scale isolation and purification of high copy and low copy plasmid DNA. The ready-to-use plasmid DNA is of high quality in low-salt Tris buffer and suitable for typical downstream applications: Cloning, sequencing, PCR, transformation and restriction analysis.
Specifications
| Parameter | High-copy Plasmid | Low-copy Plasmid |
| Max. sample volume | 1-5 ml ON-culture | 5-10 ml ON-culture |
| Typical yield | < 25 µg | < 25 µg |
| Elution volume | 50 µl | 50 µl |
| Binding capacity | 40 µg | 40 µg |
| Size of vector | < 15 kb | < 15 kb |
| Prep time | 26 min / 12 preps | 26 min / 12 preps |
| Format | spin column | spin column |
Application data:
High yield - High purity - Fast preparation
 | Fig. 1: pBluescript plasmid DNA was isolated from a 1,4 ml E.coli culture (DH5α in LB medium) according to the recommended procedures of different kits and eluted in 50 µl elution buffer. 2 µl of each eluate were loaded on a 0,7% TAE agarose gel. The gel demonstrates that FastGene® Plasmid Mini Kits yield equal amount of plasmid DNA compared to other supplier with even more time consuming protocols. The preparation with the FastGene® Plasmid Mini Kit was performed by using the Fast Protocol. |
| Fig.2: Typical fluorescent capillary sequencing result from pBluescript plasmid DNA purified using the FastGene® Plasmid Mini Kit. Cycle sequencing reaction was performed on an ABI3100 sequenzer. 1 µl of purified plasmid DNA was used as a template. Dye terminators were removed by FastGene® Dye Terminator Removal Kit |  |
Protocols - flow chart Ordering information
Ordering information
| Product | Cat.No.: | Content |
| FastGene Plasmid Mini Kit | FG-90402 | 100 preps |
| FastGene Plasmid Mini Kit | FG-90502 | 300 preps |
