FastGene® Gel/PCR Extraction Kits are designed for the extraction of DNA from agarose gels and for the purification of PCR products.
DNA fragments purified with FastGene® Gel/PCR Extraction Kits are ready for direct use in all common downstream applications, like sequencing, ligation and transformation, restriction digestion, microarray analysis, PCR and in vitro transcription.
๏ In each Kit 5 Agarose Gel Band Cutter and 50 µl Midori Green Advance DNA stain (enough to stain 1l of agarose gels) are included
The figure demonstrates that the FastGene® Gel/PCR Extraction Kit shows up to 90% of DNA recovery.
Fig. 1: PCR fragments of 300 bp were purified from 40 µl of a PCR stock solution using FastGene® Gel/PCR Extraction Kit and a competitor kit, according to manufacturers protocol. DNA was eluted in 20 µl elution buffer and 5 µl of eluate was analyzed on a 1,5% TAE agarose
The DNA recovery from agarose gels using FastGene® Gel/PCR Extraction Kits is very high even for small fragments.
Fig. 2: 10 µl of a PCR stock solution with a 100 bp amplicon were applied on a TBE agarose gel. After electrophoresis the DNA was purified using a competitor kit and the FastGene® Gel/PCR Extraction Kit, according to manufacturers protocol. DNA was eluted in 20 µl elution buffer and 10 µl of eluate was analyzed on a 2% TAE agarose gel.
Lane 1: Unpurified PCR fragment; M = Marker
Lane 2,3 and 4: Supplier M
Lane 5,6 and 7: FastGene® Gel/PCR Extraction Kit
|Parameter||Gel Extraktion||PCR Clean-up|
|Max. sample volume||300 mg Agarosegel||100 µl PCR Mix|
|Gel||< 2,5% TAE oder TBE|
|Binding capacity||10 µg||10 µg|
|DNA fragment length||50 bp - 10 kb||50 bp - 10 kb|
|Primer removal||< 25 bp|
|Elution volume||20 - 50 µl||20 -50 µl|
|Prep time||20 minutes||20 minutes|
|Format||spin column||spin column|
|FG-91202||FastGene® Gel/PCR Extraction||100 preps|
|FG-91302||FastGene® Gel/PCR Extraction||300 preps|
|FastGene® Agarose Gel Band Cutter||50 pieces|