This buffer is used to prepare samples and markers for loading on agarose or polyacrylamide gels. The 6x DNA Loading Dye contains two different dyes (Orange G and xylene cyanol FF) for visualization of DNA migration during electrophoresis. The presence of glycerol ensures that the DNA in the ladder and sample forms a layer at the bottom of the well.
Both dyes contain Xylencyanol FF. The difference between these two products is that our 5x loading dye contains Bromphenol blue instead of Orange G. Bromphenol blue is safe.