The freezing medium BambankerTM offered by the Japanese company Lymphotec was developed initially just for their own R&D projects. They needed a suitable medium for long-term storage of highly sensitive cell lines, such as lymphocytes.
Thanks to the innovative formulation of the new freezing medium, an European Patent (EP 1347040) was granted and the development of this media was made commercially available. Today this innovative cell freezing medium BambankerTM is the market leader in Japan and characterized by many different published articles with very sensitive cell lines all over the world.
Universal – even for preservation of stem cells
All common cell lines can be preserved resulting in a high number of intact cells after thawing. Recovery rates of even sensitive cells is much higher compared to regular media.
User friendly with a high stability
No dilution, no addition of components (like DMSO or glycerol). No programmable freezer and no sequential freezing steps are required – saves a lot of time. Shelf life is 24 months, when stored in a fridge (2 – 10 °C).
Because of the unique formulation, Lymphotec Inc. has got a European patent (EP 1347040).
We can offer 1 x 20, 5 x 20 and 1 x 120 ml bottles.
Stabilization of Mouse Embryonic Stem Cells
||15% FBS/DMEM (1 mM of Sodium pyruvate, 100 µM of NEAA, 100 µM of ß-ME, 1000 U/ml of LIF) was used as culture medium. Mouse Embryonic Fibroblasts (MEF) were used as „feeder cells“.
||Cells were frozen in 5 vials / ( 60mm dish corresponds to 3.0 x 106 cells/vial ). 1 ml/vial of BambankerTM freezing medium was added and the mixture was directly frozen in -80 °C. The following day, the vials were transferred to liquid nitrogen (slow freezing).
||Cells were incubated at 37 °C and transferred in cooled culture media. After collection, cells were seeded in 6 well plates and 6 cm dishes.
||Stabilization of Mouse Embryonic Stem Cells by using BambankerTM was succesful. Cells were undifferentiated, even after freeze and thaw procedure. No modifications of cells could be observed.
Data were kindly provided by Dr. Ahn (Tokyo Institute of Technology Graduate School of Bioscience and Biotechnology Department of Biomolecular Engineering, Tagawa Laboratory, Japan).
Die Daten wurden von Dr. Ahn (Tokyo Institute of Technology Graduate School of Bioscience and Biotechnology Department of Biomolecular Engineering, Tagawa Laboratory, Japan ) zur Verfügung gestellt.
Some cell lines which already were successfull tested with Bambanker:
P3U1 (mouse myeloma cell line)
K562 (human leukemia cell line)
Human gastric epithelial cell line
Human γδT cell
Daudi (human B cell line)
PC12 (rat-derived adrenal pheochromocytoma)
Human B cell line
OKT4 (mouse hybridoma cell line)
Monkey B cell line
Mouse ES cell line
Activated lymphocyte cell line derived from human peripheral blood
Activated lymphocyte cell line derived from mouse spleen
Astrocyte cell line dervied from brain tissue of newborn Wistar rats (Rattus norvegicus)
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