Bambanker (20 ml)

Article number: BB03

20 ml serum free freezing media for long storage of cells

Bambanker Gefriermedium

The freezing medium BambankerTM offered by the Japanese company Lymphotec was developed initially just for their own R&D projects. They needed a suitable medium for long-term storage of highly sensitive cell lines, such as lymphocytes.

Thanks to the innovative formulation of the new freezing medium, an European Patent (EP 1347040) was granted and the development of this media was made commercially available. Today this innovative cell freezing medium BambankerTM is the market leader in Japan and characterized by many different published articles with very sensitive cell lines all over the world.

Universal – even for preservation of stem cells

All common cell lines can be preserved resulting in a high number of intact cells after thawing. Recovery rates of  even sensitive cells is much higher compared to regular media.

User friendly with a high stability

No dilution, no addition of components (like DMSO or glycerol). No programmable freezer and no sequential freezing steps are required – saves a lot of time. Shelf life is 24 months, when stored in a fridge (2 – 10 °C).

 

Haltbarkeit von Bambanker Gefriermedium

Innovative

Because of the unique formulation, Lymphotec Inc. has got a European patent (EP 1347040).

 

Flexible

We can offer 1 x 20, 5 x 20 and 1 x 120 ml bottles.

Verpackungsgrößen von Bambanker Gefriermedium

Stabilization of Mouse Embryonic Stem Cells

Embryonale Mausstammzellen mit Bambanker Medium eingefroren

Cultivation 15% FBS/DMEM (1 mM of Sodium pyruvate, 100 µM of NEAA, 100 µM of ß-ME, 1000 U/ml of LIF) was used as culture medium. Mouse Embryonic Fibroblasts (MEF) were used as „feeder cells“.
Freezing Cells were frozen in 5 vials / ( 60mm dish corresponds to 3.0 x 106 cells/vial ). 1 ml/vial of BambankerTM freezing medium was added and the mixture was directly frozen in -80 °C. The following day, the vials were transferred to liquid nitrogen (slow freezing).
Thawing Cells were incubated at 37 °C and transferred in cooled culture media. After collection, cells were seeded in 6 well plates and 6 cm dishes.
Results Stabilization of Mouse Embryonic Stem Cells by using BambankerTM was succesful. Cells were undifferentiated, even after freeze and thaw procedure. No modifications of cells could be observed.
Data were kindly provided by Dr. Ahn (Tokyo Institute of Technology Graduate School of Bioscience and Biotechnology Department of Biomolecular Engineering, Tagawa Laboratory, Japan).
Die Daten wurden von Dr. Ahn (Tokyo Institute of Technology Graduate School of Bioscience and Biotechnology Department of Biomolecular Engineering, Tagawa Laboratory, Japan ) zur Verfügung gestellt.

Some cell lines which already were successfull tested with Bambanker:

P3U1 (mouse myeloma cell line)
K562 (human leukemia cell line)
Human gastric epithelial cell line
Human γδT cell
Daudi (human B cell line)
PC12 (rat-derived adrenal pheochromocytoma)
Human B cell line
OKT4 (mouse hybridoma cell line)
Monkey B cell line
Mouse ES cell line
Activated lymphocyte cell line derived from human peripheral blood
Activated lymphocyte cell line derived from mouse spleen
Astrocyte cell line dervied from brain tissue of newborn Wistar rats (Rattus norvegicus)

 

Hatoya, S. et al. Effect of co-culturing with embryonic fibroblasts on IVM, IVF and IVC of canine oocytes. Theriogenology 66, 1083–1090 (2006).

Hikichi, T. et al. Differentiation potential of parthenogenetic embryonic stem cells is improved by nuclear transfer. Stem Cells 25, 46–53 (2007).

Huang, Y. H., Yang, J. C., Wang, C. W. & Lee, S. Y. Dental Stem Cells and Tooth Banking for Regenerative Medicine. J. Exp. Clin. Med. 2, 111–117 (2010).

Lechner, S. M. Interaktionen von Inseminatbestandteilen mit Epithelzellen und Leukozyten im Uterus des Rindes. (2008).

Liu, D. et al. Relation between human decay-accelerating factor (hDAF) expression in pig cells and inhibition of human serum anti-pig cytotoxicity: Value of highly expressed hDAF for xenotransplantation. Xenotransplantation 14, 67–73 (2007).

Mieno, S. et al. Effects of diabetes mellitus on VEGF-induced proliferation response in bone marrow derived endothelial progenitor cells. J. Card. Surg. 25, 618–625 (2010).

Mieno, S. et al. Characteristics and Function of Cryopreserved Bone Marrow-Derived Endothelial Progenitor Cells. Ann. Thorac. Surg. 85, 1361–1366 (2008).

Naito, H. et al. The advantages of three-dimensional culture in a collagen hydrogel for stem cell differentiation. J. Biomed. Mater. Res. – Part A 101, 2838–2845 (2013).

Shimizu, Y. et al. Impaired tax-specific t-cell responses with insufficient control of HTLV-1 in a subgroup of individuals at asymptomatic and smoldering stages. Cancer Sci. 100, 481–489 (2009).

Takata, Y. et al. Generation of iPS Cells Using a BacMam Multigene Expression System. Cell Struct. Funct. 36, 209–222 (2011).

Tamai, Y. et al. Potential contribution of a novel Tax epitope-specific CD4+ T cells to graft-versus-Tax effect in adult T cell leukemia patients after allogeneic hematopoietic stem cell transplantation. J. Immunol. 190, 4382–92 (2013).

Zaidi, S. K. et al. Runx2 deficiency and defective subnuclear targeting bypass senescence to promote immortalization and tumorigenic potential. Proc. Natl. Acad. Sci. U. S. A. 104, 19861–19866 (2007).

DownloadsApplication Note - JCRB
Bambanker Test und Resultate der JCRB Zellbank für 4 besonders schwer kultivierbare Zelllinien
MSDS
Material Safety Data Sheet
Protocol
Protocol for cell freezing
Bambanker Stem-cells flyer
Bambanker Stem-cells flyer
Cell biology Products
Bambanker Flyer

on 07/23/2018

Mit der Verwendung des Bambaker Einfriermediums konnten wir den prozentualen Anteil überlebender iPS-Zellen nach dem Auftauen wesentlich erhöhen. Wir sind mit diesem Produkt überaus zufrieden und verwenden es mittlerweile für alle Stammzellkulturen.

Translation by Nippon Genetics:

By using the Bambaker freezing medium, we were able to increase significantly the percentage of surviving iPS cells after thawing. We are very satisfied with this product and now we use it for all our stem cell cultures.

on 02/02/2018

Since many years we are working with primary cell cultures derived from high-grade brain tumors which are highly susceptible to environmental changes and differing culture conditions. Thus, freezing and thawing is a challenging process for these cells. According to our experiences, we could not avoid to lose 30% to 40% of living cells after each freezing/thawing cycle. In addition, the cells need a recovery time of at least five days after freezing in our previous freezing medium, obtained from other manufacturers. We’ve recently tested the Bambanker medium with striking results: recovery times of only two days of 80% of living and “healthy” cells. We observed this improvement of recovery time and proportion of living cells with all four cell lines, that we’ve tested so far. We are very satisfied with Bambanker and will continue to use it.

Translated by Nippon Genetics:

Wir arbeiten seit vielen Jahren mit primären Zellkulturen, die aus weitfortgeschrittenen Gehirntumoren gewonnen werden. Diese Zellen reagieren sehr empfindlich auf Umweltveränderungen und sich ändernde Kulturbedingungen. Das Einfrieren und Auftauen wird damit zu einem schwierigen Prozess für diese Zellen. Unseren Erfahrungen nach können wir einen Verlust von 30 % bis 40 % der lebenden Zellen nach jedem Einfrieren/Auftauen Zyklus nicht vermeiden. Zusätzlich benötigen die Zellen nach dem Einfrieren in unserem vorherigen Medium eines anderen Herstellers eine Erholzeit von mindestens fünf Tagen. Kürzlich haben wir das Bambanker Medium mit faszinierenden Resultaten getestet: Erholzeiten von nur zwei Tagen von 80 % der lebenden und "gesunden" Zellen. Wir konnten diese Verbesserung der Erholzeit und die Proportion von lebenden Zellen bei allen vier Zelllinien, die wir bisher getestet haben, beobachten. Wir sind sehr zufrieden mit Bambanker und werden es weiterhin benutzen.

on 10/24/2017

“JCRB cell bank has carried out cell bank business for 30 years and we currently store 1400 types of cell lines. In 2013, we offered about 4,300 ampules for a fee to domestic and foreign researchers. Due to the high number and the wide variety of cell lines, we had some problems. Thus some users complained that their cell lines of dying after thawing, resulting in unsuccessful cultivation. Especially four types of cell lines were a problem which had to be urgently improved. Therefore, we compared Bambanker™ with our currently used commercial preservation medium in a cryopreservation test. The cell lines, which were stored with Bambanker™, showed much higher cell proliferation than cells, which were stored with our currently used commercially available product. Surprisingly, with Bambanker™ we got for all four cell lines very reproducible results. In addition, Bambanker has no differences between the lots since it does not contain serum. We are very grateful for that because it strongly simplifies the delivery procedure overseas. In the future, we will completely change to Bambanker™ in order to improve the survival rate and growth of our cells. We are thankful for resolving that long-standing problem and recommend Bambanker™ to all domestic researchers and foreign cell banks. This data will be also described at our JCRB website. In the near future we plan to test Bambanker™ for human tissue-derived and human iPS cell lines.”
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