BlueStar Prestained Protein Marker (500 µl)

Catalogue number: MWP03

1 x 500 µl Protein Marker in loading buffer, sufficient for 100 mini gels or 50 large gels

Broad range: 10 – 180 kDa

BlueStar Prestained Protein Marker

The BlueStar Prestained Protein Ladder is a three color protein standard with 10 prestained proteins covering a wide range molecular weights from 10 to 180 kDa. The BlueStar Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon or nitrocellulose) and for approximating the size of proteins.

 

Lauferhalten dse BlueStar Proteinmarkers in Tris-Glycin, Bis-Tris MOPS und Bis Tris MES Puffer

 

  • Broad range: 10 – 180 kDa
  • High performance: 10 bands with 3 colours (blue, orange and green)
  • Ready to use: Supplied in a loading buffer for direct loading on gels.
  • Reference bands: 28 kDa (green) and 75 kDa (orange)
  • Sharp bands

 

Storage Buffer

20 mM Disodium hydrogen phosphate pH 8.0 (at 25°C ), 2 % SDS, 0.2% Triton X-100,10 mM 2-Mercaptoethanol, 6 M Urea and 27% (v/v) Glycerol.

 

Storage

Stable at -20°C for 12 months.
Stable at +4°C for 3 months.

 

Recommendations for Loading

1. Thaw the ladder either at room temperature or at 37-40°C for a few minutes to dissolve precipitated solids. Do not boil.
2. Mix thoroughly, to ensure that the solution is homogeneous.
3. Load the following volumes of the ladder on SDS-polyacrylamide gel:

  • 5 μl per well for mini-gels, 3 μl per well for blots
  • 10 μl per well for large gels, 6 μl per well for blots

Applications

• Monitoring of protein migration during SDS-polyacrylamide gel electrophoresis

• Monitoring of protein transfer onto membranes during Western blotting

• Sizing of proteins on SDS-polyacrylamide gels and Western blots

Manual

MWP03_Manual

 

Safety Data Sheet (MSDS)

MWP03_MSDS

Does this marker contain Formaldehyde or Formamide?

No.

on 02/22/2018

Very good product. The range of the protein ladder is for our purposes perfect. Nicely visible protein ladder. We use it as our standard protein ladder for gel electrophoresis.

Translation by Nippon Genetics:

Sehr gutes Produkt. Der Bereich der Protein Ladder ist für unsere Zwecke perfekt. Eine gut sichtbare Protein Ladder. Wir nutzen diesen Protein Marker als unseren Standard für die Gelelektrophorese.

on 02/08/2018

We have been using Nippon Genetics protein markers in our laboratory for the past 7 years. They are easy to use and exceptionally cost-efficient. The range of the molecular weights is very broad so we can use them for all of our SDS-polyacrylamide gel applications. The bands are sharp and focused and very easily identifiable by the reference colors. Overall, we are extremely satisfied with the quality of these markers.

Translation by Nippon Genetics:

Wir benutzen die Protein Marker von Nippon Genetics seit 7 Jahren in unserem Labor. Sie sind leicht anzuwenden und besonders preisgünstig. Der Molekulargrößenbereich ist sehr umfassend, so dass wir die Marker für alle unsere SDS-Polyacrylamidgelapplikationen benutzen können. Die Banden sind scharf, klar und durch die Referenzfarbbanden sehr einfach identifizierbar. Insgesamt sind wir sehr zufrieden mit der Qualität dieser Marker.
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