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FAS Digi Gel Documentation System

Article number: GP-05LED

FastGene® FAS Digi imaging system: Gel documentation system with Blue/Green LED technology

DNA Detection and Documentation with red or green DNA dyes

The FastGene® Blue/Green LED technology enables the detection of red dyes, such as Ethidium Bromide and of our next generation green dyes Midori Green. Both dyes can be visualized using the amber filter on top of the unit.

New LED Technology

Figure 1 – The absorbance spectrum of Ethidium Bromide (red), Midori Green Advance (green) and Midori Green Direct (light green)

See the sharpest bands, even at low signals

The FastGene® FAS Digi Imaging System uses the Panasonic GF7 digital camera. Its lens has an optimal zoom range (24-128 mm) and has a big aperture (F/3.55), therefore allowing bright bands, even in the darkest situations.

Very easy to handle and perfect results out of the box

Get great results right after you start. The simplicity of the system guaranties a quick learning process. Images are saved in the established jpeg format, allowing you to use any imaging program to review your image.

Advanced options are available

The Panasonic GF7 is able to record images in a RAW format, so that advanced users can get the most information out of the images.

Robust and Durable

The FastGene® FAS Digi Geldocumentation System is very robust due to its metal body and high quality components.

Specifications

 

CameraDigital camera with a CMOS Sensor, 12-16 Megapixel and 3″ LCD Monitor
LensZoom range 24-64 mm, (Fullframe), Aperture f/3.5-f/5.6
Size34 x 45 x 27 cm (length x height x depth)
Weight6.3 kg
IlluminatorFastGene® Blue/Green Transilluminator
Optical filterAmber filter
Compatible printerP93E
Power supplyBlue/Green Transilluminator:

• input: 100-240 V, 50-60 Hz (24 V, 1 A)
• output: 24 V, 1 A

Pentax Battery Charger:

• input: 110-240 V, 50-60 Hz
• output: 4.2 V, 0.8 A


 

Access your gel and cut your DNA Bands easily thanks to the separable components

 

Images taken with the FastGene® FAS Digi Imaging System

FAS-Digi and midori green advance

Figure 1 – PCR-Products separated on a 1% TBE-Agarosegel. The DNA stain used was the Midori Green Advance.

Fas-Digi and Midori Green Direct

Figure 2 – PCR-Products separated on a 1% TBE-Agarosegel.The DNA stain used was the Midori Green Advance.

Downloads

Manual

Imaging Broschüre
Geldokumentationssysteme mit einzigartiger blau/grün LED Technologie

FAS Digi - P93E Printer

How does the Fas Digi work with a printer? Does this use the same cord as would be used to connect the camera to a computer? Can you also connect the system to a computer and printer at the same time? Is it possible to plug in old printers (e.g. P90)?

You can connect a printer. See the protocol which you can find in the download section. There are different cables for connecting a computer or a printer. You can connect the pc to a printer and the camera the same time, but you can’t connect the camera to the printer and computer the same time. It is possible to connect the old printer, if it has an analogue signal.


 

We want to stain microplastic by nile-red and want to count the fluorescent particles. We know that it is possible to activate nile-red by UV, so we wanted to know if this is also possible by the FasDigi?

We do not have direct information regarding the performance of nile-red and the FasDigi. The absorption maximum is at 559nm. So really far to green nearly yellow, which is not optimal for the FasDigi. But we know that we can detect mCherry, which has its maxima at 587nm.


 

How can I ensure that my bands are not overexposed?

The camera has an overexporsure alarm. To set this alarm go to the menu custom settings page 4/7 there is the menu highlight. Turning that on will highlight in black if a pixel is overexposured. If you want to see it in the live view there is a zebra function in the camera where the saturation value can be adjusted from 50% saturated to 105% saturated (oversaturated). Page 188 in the manual has a full description of the function.


 

I want to perform densitometry on the images and prefer to use the images in reverse “negative” mode. Is this possible to set it up in the camera?

I do not think that this mode is possible with this camera, but any software on the computer should be able to do.


 

Is it possible to chain the camera to the system?

No, we recommend to lock the camera with the adapter in a draw when it is not used. Normally there are always lockable draw in a lab.


 

How large is the SD card on the camera?

8GB


 

What type of images are recorded?

The GF7 records jpeg and RAW at the same time.


 

In the Wifi mode, is the image sent to the device which is directly connected?

That’s correct. Multiple connections with more than one devices is not possible at the same time.


 

Can you reduce the image size of the jpegs images?

There are three different sizes of jpeg files. You will find them here: Press the menu. Then choose the camera symbol, which you can see on the top of the left symbol column (you can choose it with the menu/set button in the middle of the ring on the right camera side). Then choose “picture size”. There are three options L= 12M, M=8M and S=2M.


 

Do you need special software for saving images via cable to a PC?

No, there is no special Lumix software necessary. You can connect the camera with the PC and open the pictures with standard programs. You will find a folder with the pictures which you can save on your computer and then open and edit with your own program.


 

Can I use ethidium bromide or any other nucleic acid staining dye other than midori green in this gel doc system? Do I need to buy any extra optical filter?

You can use EtBr, GelRed and many others. You can always use the same filter.


 

How can I transfer images to the computer?

There is a USB cable and you can use the SD card.


 

How is the durability of the gel doc system?

We have systems running since 2014 without problems.


 

How is the lifetime of the blue green light?

The LEDs have a life experience of 50000h (more than 24 years).


 

How to charge the camera?

The camera comes with a battery charger. So you have to load the batterie from time to time.


 

Can I connect the Fas-Digi with a screen?

You can connect the camera to a computer monitor by using a Micro-HDMI to HDMI cable. But then you only can see images which you shot with the camera and no live view. There is also an App (Panasonic Image App)by which you can control the camera on a Tablet or mobile phone. Here you can see the live view image and shoot pictures.


Can you digital beyond zoom (beyond the manual zoom) with the camera?

Yes you can! It is in the menu of the camera settings. It is described on page 195 of the manual.

 


Does the camera has to be fixed by the screw?

No, please remove the screw form the dark box. The screw is only added to secure the camera while the system is not in use.

on 09/27/2017

"Our research group purchased a FAS-Digi, being composed of a Blue/Green LED transilluminator, hood and camera, in October 2014. It replaced a much larger gel documentation, which had UV-light and was being used with EtBr. The old gel doc system had more function but these functions were not being used. We enjoyed the fact that the FAS-Digi was a compact gel documentation system. Due to its size and changing to an alternative dye, we placed it next to our mini-gel tanks. We are using “DNA-Dye Non Tox” from AppliChem. This dye can be directly added to the sample making it possible to cut the unused part of the gel and storing it for later. The FAS-Digi is manageable and useful and is enough for our applications. We are using the system for the documentation of DNA mini and maxi gels (mainly PCR and analysis of restriction digests of mini-preps), as well as the white light table for SDS-gels. There is a possibility to move the camera backwards, enabling a look through the build-in amber filter to check if a gel worked without having to turn on the camera. Additionally, the included amber goggles can be used to excise pieces of the gel for cloning, which works great (both the excision of gels and the cloning). The sensitivity is at least as with the old UV-light system, although we were not able to perform an exact test. The regular consumer camera, goes back to its original setup even if a user has changed it previously. We have changed the settings to include a date setting when taking an image. The gel documentation system is working for the last 2.5 years and does show only little signs of wear-off. We have not connected it a laptop but to save an image we remove the SD-card instead and use a normal card reader on a PC. Additionally, I back up all the images every 6 months. Until now, the back up was not necessary. There are some drawbacks. The system should be placed on a leveled surface. Otherwise, the door opens very quickly and hits the back part the instruments. Many users also believe that the cable coming from the back powers the camera. This is not the case, since it only powers the nicely working LED-table. The camera has its own battery which lasts for several months. I remove the battery occasionally to charge it with the included charger. In the end, we can say that the purchase was not regretted and that we would buy the system again."

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