FAS V Gel Documentation System (CCD-Sensor)

Article number: GP-FAS-V

Price on request

FastGene® FAS V – Gel documentation system with Blue/Green LED technology – for documentation of DNA/RNA in agarose gels, protein gels and petri dishes

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 DNA Detection and Documentation with red or green DNA dyes

The FastGene® Blue/Green LED technology enables the detection of red dyes, such as Ethidium Bromide and of our next generation green dyes Midori Green. Both dyes can be visualized using the amber filter on top of the unit.

Unique LED Technology

Figure 1 – The absorbance spectrum of Ethidium Bromide (grey), Midori Green Advance (purple) and Midori Green Direct (green)

Very easy to handle and perfect results out of the box

Get great results right after you start. The simplicity of the system guarantees a quick learning process. Images are saved in the established jpeg and tiff format format, allowing you to use any imaging program to review your image. Additionally, the images can be saved in PNG and BMP format as well.

Robust and Durable

The FastGene® FAS-V Gel documentation System is very robust due to its metal body and high quality components.

Touchscreen Operation

touchscreen

The FastGene® FAS-V is controlled by a 10.4″ touchscreen display:

  • All three light sources can be activated and deactivated by the touchscreen.
  • The exposure time and Gain can be adjusted slide function or by directly adding the desired value.
  • Capturing the image can be achieved easily by pressing capture

6 pictures simultaneously

The FastGene® FAS-V will not only take 1 picture but 6 simultaneously using different exposure times. The user can then decide which one to use.

different image formats

The chosen image is then saved in JPEG, TIFF, BMP or PNG-format (BMP is selected in the image)

Blue/Green LED Transilluminator

Blue/Green LED technology enables the detection of red and green DNA dyes. The FastGene® FAS-V has the biggest Blue/Green LED Transilluminator yet. The huge imaging area is 26 cm x 21 cm big and has a superb uniform transillumination. This is guaranteed by 12 LED arrays at each side.

White LED Transilluminator

The detection of protein is possible due to the very large white LED table (26 cm x 21 cm). This table can be directly controlled using the touchscreen.

White LED Room light

The documentation of membranes and Petri dishes is done by switching on the room light LED. Two perfectly placed LED arrays illuminate the whole imaging box. Black or white background can be chosen to increase or decrease the contrast.

Very sensitive CCD-Sensor

The FastGene® FAS-V has a big CCD-Sensor, made in Japan, with a diameter of 1/1.8“. The pixel size of 4.4 µm allows the detection of lowest light signal. Images can be recorded in the common TIFF and JPEG as well as in BMP and PNG format. The files can be stored in the 16 GB internal SSD storage or on a USB-stick.

The exposure time of the sensor can be set from 0.001 sec up to 30 sec. The very short exposure guarantees that even the brightest DNA band will be still within the dynamic range. Exposing the sensor for up to 30 sec will enable the detection even of the faintest bands.

Very bright parfocal lens – no more focusing:

The lens of the FastGene® FAS-V is parfocal, as most microscopic lenses and prefocused on the imaging area. This enables you to zoom in to the area of interest without having to readjust the focus.

The huge aperture* of f/1.2 enables the transmission of light to the sensor. The aperture can be regulated steplessly until complete closure, making it your decision which aperture delivers the best image.

The 6 x zoom, with a focal length of 12.5 mm to 75 mm, allows a perfect enlargement of the area of interest. Setting the lens at 12.5 mm allows the imaging of the complete illuminated area, while zooming into 75 mm will eliminate any unnecessary area.

*opening of the lens iris

 

Storage

The internal 16 GB storage can be expandable using common USB-sticks.

Network

Die FastGene® FAS-V besitzt einen 100 MBits Ethernetport.

Painting

The FastGene® FAS-V comes with the driver for the Mitsubishi P95DE driver preinstalled, for perfect gel documentation on side.

Solicit a quotation for the FAS-V in combination with the Mitsubishi P95DE printer.

It is also possible to purchase the printer at a later date.

Recorded images can be directly edited in the FastGene® FAS-V:

  1. The image can be cropped using your finger or a stylus.
  2. Increase the contrast of the image
  3. The image can be rotated clockwise and anti-clockwise
  4. The image can be vertically and horizontally mirrored
  5. Reduce printing costs by inverting the image

Camera 2 MPixel 1/1,8″ CCD Sensor
Lens Parfocal, Zoom range 12,5-75 mm, (Fullframe), Aperture f/1,2
Size 78,5 x 40 x 38,2 cm
Touchscreen 10,4″ Screen Technology for Finger and Stylus Input
Magnification 6x optical zoom
Data storage Internal storage + external via USB
Connections Connection to Mitsubishi Thermoprinter P95DE

Ethernet 100 MBit

3 x USB

Weight 35 kg
Illuminators 1. FastGene® Blue/Green LED Transilluminator XL (21 x 26 cm)
2. FastGene® White LED Transilluminator XL (21 x 26 cm)
3. FastGene® White LED Room Light
Optical Filter Amber Filter
Power Supply
  • Input: 100-240 V, 50-60 Hz
  • Output: 24 V, 2 A

 

Downloads

Imaging Broschüre
Geldokumentationssysteme mit einzigartiger blau/grün LED Technologie

Manual
Handbuch der FAS-V (auf Englisch)

QuickGuide
Kurze Beschreibung für die Bedienung der FAS-V

CE Declaration of Conformity

FCC Declaration of Conformity
Zertifikat gemäß den EMV- und funkspezifischen Anforderungen der Federal Communications Commission (FCC)

What is the benefit of CCD camera?

A CCD sensor has the highest sensitivity and a linear signal acquisition, enabling quantification of DNA Bands.


 

The FasV has a 2mp camera and other have 5mp. Isn’t that much better?

The resolution is not really important, as the gel sizes are not big. A detailed image is guaranteed by the zoom, enabling the documentation of single bands. The more important feature is the sensitivity of the sensor, which is superb at the FAS-V (0.13 Lux, Normal dy light 100,000 Lux).


 

How flexible is the software?

The editing software can label, crop, change contrast, rotate and mirror the image, invert, print the image. There is also a quantification software as separate product. This software can analyse and quantify the bands documented by the FAS-V.


 

What are the parameters than can be analyzed with?

Quantification software: Automatic removal of background, automatic lane and band determination, determination of molecular size when compared to a known standard, determination of signal intensity and quantification of DNA/Protein when compared to a known standard.


 

What is the benefit of a removable filter?

DNA and proteins can be recorded. Our filter is comparable with green DNA dyes (Midori) or red DNA dyes (EtBr). To document protein gels, the filter has to be removed (very easy).


 

Is it possible to set the device up using DHCP for networking?

The FAS-V has no DHCP setting function. It is required to setup a fixed IP. Please make sure the mask and gateway IP is also set up properly.


 

Is it possible to have different user accounts?

It is not possible to have different user accounts. But you can create for every user an individual folder, so that every user will have an overview over his pictures.


 

Does the Fas-V has a sleeping mode or do I have to switch it off?

The Fas-V switches in sleeping mode. You can set the time in the operating menu.


 

Is the glass on the Blue/Green Transilluminator replaceable?

The glass itself is not replaceable since we need to make it watertight. There is however the option of replacing the table should anything happen. Small hint from my side if the agarose gel band cutter is not an option: Purchasing an acrylic glass plate from the local DYI-store and using it as a cutting board on top of the illuminator might do the trick.


 

Q: Can the system be connected to the computer directly?

GP-FAS-V can be connected via network, but only to save images.


 

Q: Can the system be connected to a printer?

A: Yes, to the P95DE


 

on 05/29/2018

FAS-V imager is a very convenient device with great optics and manual controls over image acquisition. Of special note is a sensitive camera producing sharp images in a wide range of signal intensities.

Main convenience over other imagers is high sensitivity and low background, thus, allowing economise on gel stains (2-5 time less can be used); and the manual adjustment of the focus, the zoom and the orifice. Since all the adjustment rings are close to each other, it all can be set with one hand and with immediate effect observed on the screen. Additional digital adjustments with Gain and Gamma options also helpful to get the most from the image. Adjustable “Image size” option gives is very convenient to accommodate gels of various sizes.

In our department, FAS-V is used for imaging of DNA/RNA gels stained with SybrSafe and for Coomassie protein gels. In addition, we found it very useful for screening fluorescent GFP –tagged vs non-tagged colonies on agar surface. The imager is also capable of imaging bioluminescent bacteria (equipped with bacterial luciferase LuxAB) as both colonies on agar surface and in liquid in quantitative manner.

I am very satisfied and I would certainly recommend FAS-V for other users.

This imager can be further improved though:

1. The screen angle has to be more “horizontal” to be well seen from the height of an average person.

2. Extra USB ports needed to accommodate more than one USB connected devices: wireless mouse and memory stick

To give more functionality: Exchangeable filters (perhaps a revolver) can be added to allow for imaging not only GFP but also mCherry and, probably, other fluorescent proteins, many users asked for it.

on 01/11/2018

Wir verwenden das Gerät Fas-V um z.B PCR-Produkte sichtbar zu machen. Hierbei verwenden wir den Farbstoff SYBR Safe. Jedoch verwenden wir das Gerät nicht nur um DNA, sondern auch RNA sichtbar zu machen. Diese werden mit Ethidiumbromid angefärbt. Sowohl SYBR Safe als auch Ethidiumbromid stellen kein Problem für das Gerät Fas-V dar. Was wir persönlich an dieser Technologie so toll finden ist zum einen, dass das Blau-Grünlicht kaum die DNA schädigt und zum anderen, dass es nicht gefährlich für die eigene Person ist. Mit der Filterbrille kann man direkt auf das Gel schauen und beurteilen ob z.B. die PCR erfolgreich war, ohne das Gel erst fotografieren zu müssen. Auch die integrierte Weißlichtplatte ist sehr praktisch. Bei Bedarf klappt man diese einfach an der Seite herunter und zieht sie hervor. Somit erspart man sich das Umstecken von externen Platten. Ein Bild von einem Film oder z.B. einer Platte ist schnell gemacht (allerdings nur in schwarz-weiß). Die Bedienung des Geräts über das Touchpad ist sehr modern und angenehm. Datum, Uhrzeit, Titel und auch Bildeinstellungen werden auf dem Bild abgebildet, wenn man das möchte. Ein kleiner zusätzliches Thermoprinter ermöglicht ein schnelles Festhalten der Ergebnisse im Laborbuch! Ein super kompaktes und benutzerfreundliches Gerät, welches sehr zu empfehlen ist!

Translation by Nippon Genetics:

We use the FAS-V gel documentation system to visualize PCR-products. For that we use the DNA dye SYBR safe. Additionally, besides DNA we also use the system to visualize RNA, which was stained with ethidium bromide. Both dyes are no problem for the FAS-V gel documentation system. Personally, the two biggest advantages of the Blue/Green light is that the light is not damaging the DNA and is not dangerous for the user. Using the included filter goggles, it is possible to determine the success of the PCR without having to take an image of the gel first. The white light LED illuminator is very handy too. When needed, the plate is turned down and positioned centrally without having to reconnect external illuminator and taking an image of an X-ray film or dish is very easy (although only monochrome). The handling of the system using the touchscreen is pretty modern and comfortable. Date, time, a title and the image recording settings are saved on the image, if one wants to have it. An additional thermoprinter does the documentation in a lab journal very quick. A super compact and user-friendly system, which we heavily recommend!

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