DNA Detection and Documentation with red or green DNA dyes
The FastGene® Blue/Green LED technology enables the detection of red dyes, such as Ethidium Bromide and of our next generation green dyes Midori Green. Both dyes can be visualized using the amber filter on top of the unit.
Unique LED Technology
Figure 1 – The absorbance spectrum of Ethidium Bromide (grey), Midori Green Advance (purple) and Midori Green Direct (green)
Very easy to handle and perfect results out of the box
Get great results right after you start. The simplicity of the system guarantees a quick learning process. Images are saved in the established jpeg and tiff format format, allowing you to use any imaging program to review your image. Additionally, the images can be saved in PNG and BMP format as well.
Robust and Durable
The FastGene® FAS-V Gel documentation System is very robust due to its metal body and high quality components.
The FastGene® FAS-V is controlled by a 10.4″ touchscreen display:
- All three light sources can be activated and deactivated by the touchscreen.
- The exposure time and Gain can be adjusted slide function or by directly adding the desired value.
- Capturing the image can be achieved easily by pressing capture
The FastGene® FAS-V will not only take 1 picture but 6 simultaneously using different exposure times. The user can then decide which one to use.
The chosen image is then saved in JPEG, TIFF, BMP or PNG-format (BMP is selected in the image)
Blue/Green LED Transilluminator
Blue/Green LED technology enables the detection of red and green DNA dyes. The FastGene® FAS-V has the biggest Blue/Green LED Transilluminator yet. The huge imaging area is 26 cm x 21 cm big and has a superb uniform transillumination. This is guaranteed by 12 LED arrays at each side.
White LED Transilluminator
The detection of protein is possible due to the very large white LED table (26 cm x 21 cm). This table can be directly controlled using the touchscreen.
White LED Room light
The documentation of membranes and Petri dishes is done by switching on the room light LED. Two perfectly placed LED arrays illuminate the whole imaging box. Black or white background can be chosen to increase or decrease the contrast.
Very sensitive CCD-Sensor
The FastGene® FAS-V has a big CCD-Sensor, made in Japan, with a diameter of 1/1.8“. The pixel size of 4.4 µm allows the detection of lowest light signal. Images can be recorded in the common TIFF and JPEG as well as in BMP and PNG format. The files can be stored in the 16 GB internal SSD storage or on a USB-stick.
The exposure time of the sensor can be set from 0.001 sec up to 30 sec. The very short exposure guarantees that even the brightest DNA band will be still within the dynamic range. Exposing the sensor for up to 30 sec will enable the detection even of the faintest bands.
Very bright parfocal lens – no more focusing:
The lens of the FastGene® FAS-V is parfocal, as most microscopic lenses and prefocused on the imaging area. This enables you to zoom in to the area of interest without having to readjust the focus.
The huge aperture* of f/1.2 enables the transmission of light to the sensor. The aperture can be regulated steplessly until complete closure, making it your decision which aperture delivers the best image.
The 6 x zoom, with a focal length of 12.5 mm to 75 mm, allows a perfect enlargement of the area of interest. Setting the lens at 12.5 mm allows the imaging of the complete illuminated area, while zooming into 75 mm will eliminate any unnecessary area.
*opening of the lens iris
The internal 16 GB storage can be expandable using common USB-sticks.
The FastGene® FAS-V has a 100 MBits Ethernetport.
The FastGene® FAS-V comes with the driver for the Mitsubishi P95DE, for perfect gel documentation on side.
Solicit a quotation for the FAS-V in combination with the Mitsubishi P95DE printer.
It is also possible to purchase the printer at a later date.
Recorded images can be directly edited in the FastGene® FAS-V:
- The image can be cropped using your finger or a stylus.
- Increase the contrast of the image
- The image can be rotated clockwise and anti-clockwise
- The image can be vertically and horizontally mirrored
- Reduce printing costs by inverting the image
||2 MPixel 1/1,8″ CCD Sensor
||Parfocal, Zoom range 12,5-75 mm, (Fullframe), Aperture f/1,2
||78,5 x 40 x 38,2 cm
||10,4″ Screen Technology for Finger and Stylus Input
||6x optical zoom
||Internal storage + external via USB
||Connection to Mitsubishi Thermoprinter P95DE
Ethernet 100 MBit
3 x USB
||1. FastGene® Blue/Green LED Transilluminator XL (21 x 26 cm)
2. FastGene® White LED Transilluminator XL (21 x 26 cm)
3. FastGene® White LED Room Light
- Input: 100-240 V, 50-60 Hz
- Output: 24 V, 2 A
What is the benefit of CCD camera?
A CCD sensor has the highest sensitivity and a linear signal acquisition, enabling quantification of DNA Bands.
The FasV has a 2mp camera and other have 5mp. Isn’t that much better?
The resolution is not really important, as the gel sizes are not big. A detailed image is guaranteed by the zoom, enabling the documentation of single bands. The more important feature is the sensitivity of the sensor, which is superb at the FAS-V (0.13 Lux, Normal dy light 100,000 Lux).
How flexible is the software?
The editing software can label, crop, change contrast, rotate and mirror the image, invert, print the image. There is also a quantification software as separate product. This software can analyse and quantify the bands documented by the FAS-V.
What are the parameters than can be analyzed with?
Quantification software: Automatic removal of background, automatic lane and band determination, determination of molecular size when compared to a known standard, determination of signal intensity and quantification of DNA/Protein when compared to a known standard.
What is the benefit of a removable filter?
DNA and proteins can be recorded. Our filter is comparable with green DNA dyes (Midori) or red DNA dyes (EtBr). To document protein gels, the filter has to be removed (very easy).
Is it possible to set the device up using DHCP for networking?
The FAS-V has no DHCP setting function. It is required to setup a fixed IP. Please make sure the mask and gateway IP is also set up properly.
Is it possible to have different user accounts?
It is not possible to have different user accounts. But you can create for every user an individual folder, so that every user will have an overview over his pictures.
Does the Fas-V has a sleeping mode or do I have to switch it off?
The Fas-V switches in sleeping mode. You can set the time in the operating menu.
Is the glass on the Blue/Green Transilluminator replaceable?
The glass itself is not replaceable since we need to make it watertight. There is however the option of replacing the table should anything happen. Small hint from my side if the agarose gel band cutter is not an option: Purchasing an acrylic glass plate from the local DYI-store and using it as a cutting board on top of the illuminator might do the trick.
Q: Can the system be connected to the computer directly?
GP-FAS-V can be connected via network, but only to save images.
Q: Can the system be connected to a printer?
A: Yes, to the P95DE