The FastGene® apTaq DNA-Polymerase is a recombinant and thermostable Taq-Polymerase using the aptamer based Hot Start activation technology. The aptamer allows a reversible and immediate activation of the polymerase, leading to specific priming and a very fast PCR.
The FastGene® apTaq DNA-Polymerase is a Hot Start enzyme, which is completely inactive during room-temperature and become active only after heating. Random primer annealing and unspecific amplification that can occur with standard PCR enzymes are problems of yesterday. In contrast to antibody-based methods, the apTaq DNA-Polymerase contains synthetically manufactured aptamer-oligonucleotides. At low temperatures the polymerase is inactivated through a reversible tight bond of the aptamer. Interestingly, the aptamer acts as a molecular switch, changing its tertiary structure at higher temperatures. Temperatures below 45° C inactive the polymerase, whereas temperatures above 60 °C fully activated the enzyme. Therefore, the FastGene® apTaq DNA-Polymerase is less temperature-sensitive and reduce the risk of contamination.
The polymerase aptamer-oligonucleotide mixture is a reversible, temperature-dependent Hot Start system.
The FastGene® apTaq HotStart Polymerase is ideally suited for: