The FastGene® BAC-free HS Taq DNA Polymerase is based on the single-subunit, wild-type Taq DNA polymerase of the thermophilic bacterium Thermus aquaticus. It is however not a bacterial recombinant protein but purified from a eukaryotic expression system.
Performing PCR with bacterial templates could lead to a false positive, when using Taq enzymes purified from E. coli expression systems due to a contamination of the Taq enzyme with prokaryotic genomes. The FastGene® BAC-free HotStart Taq DNA Polymerase is produced using eukaryotic cells. Hence, no bacterial genome is present.