FastGene Dye Terminator Removal Kit (50)

Catalogue number: FG-9411

FastGene® Dye Terminator Removal Kit removes dye terminator molecules from sequencing samples using an efficient and reasonably priced gel filtration. The Kit includes a bottle of gelfiltration matrix, resuspension buffer and filter spin columns. At first an aliquot of the matrix will be mixed with resuspension buffer. Thereafter the equilibrated matrix is transferred into filter spin columns. After a brief spin, up to 20 µl of the sequencing reaction can be loaded onto the column. The last centrifugation step will elute the purified sample. All kit components can be stored at room temperature.

Application data

The DNA purified by the FastGene® Dye Terminator Removal Kit shows a very good performance in sequencing experiments, no dye blobs occur.

Sequencing result after removal of dye terminator

Fig. 1: 20 µl sequencing reaction including 2,5 µl BigDye Terminator v3.1 was purified with FastGene® Dye Terminator Removal Kit and analyzed on an ABI 3100 sequencer.

Parameter Dye Terminator Removal
Max. sample volume 20 µl sequencing reaction
Recovery > 90%
Prep time 5 minutes
Store at room temperature

Quickguide of the FastGene Dye Terminator Removal Kit

on 10/02/2017

Fast and easy to use. Before injection, we dry the purified samples with SpeedVac (without heating) and resuspend in HiDi formamide.
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