FastGene® Gel/PCR Extraction Kits are designed for the extraction of DNA from agarose gels and for the purification of PCR products.
DNA fragments purified with FastGene® Gel/PCR Extraction Kits are ready for direct use in all common downstream applications, like sequencing, ligation and transformation, restriction digestion, microarray analysis, PCR and in vitro transcription.
๏ In each Kit 5 Agarose Gel Band Cutter and 50 µl Midori Green Advance DNA stain (enough to stain 1l of agarose gels) are included
The figure demonstrates that the FastGene® Gel/PCR Extraction Kit shows up to 90% of DNA recovery.
Fig. 1: PCR fragments of 300 bp were purified from 40 µl of a PCR stock solution using FastGene® Gel/PCR Extraction Kit and a competitor kit, according to manufacturers protocol. DNA was eluted in 20 µl elution buffer and 5 µl of eluate was analyzed on a 1,5% TAE agarose
The DNA recovery from agarose gels using FastGene® Gel/PCR Extraction Kits is very high even for small fragments.
Fig. 2: 10 µl of a PCR stock solution with a 100 bp amplicon were applied on a TBE agarose gel. After electrophoresis the DNA was purified using a competitor kit and the FastGene® Gel/PCR Extraction Kit, according to manufacturers protocol. DNA was eluted in 20 µl elution buffer and 10 µl of eluate was analyzed on a 2% TAE agarose gel.
Lane 1: Unpurified PCR fragment; M = Marker
Lane 2,3 and 4: Supplier M
Lane 5,6 and 7: FastGene® Gel/PCR Extraction Kit
|Parameter||Gel Extraktion||PCR Clean-up|
|Max. sample volume||300 mg Agarosegel||100 µl PCR Mix|
|Gel||< 2,5% TAE oder TBE|
|Binding capacity||10 µg||10 µg|
|DNA fragment length||50 bp – 10 kb||50 bp – 10 kb|
|Primer removal||< 25 bp|
|Elution volume||20 – 50 µl||20 -50 µl|
|Prep time||20 minutes||20 minutes|
|Format||spin column||spin column|
A: We do not sell GP1 separately or as a Buffer Set.
The elution buffer GP3 contains just 10 mM Tris Buffer pH8 and no EtOH or EDTA.
You can use water but we would recommend to use the GP3 buffer. The pH should be between 7 and 8. If you buy water this should be OK. Please check the elution efficiency. If this is not OK check the pH of the water or use GP3.