FastGene MagnaStand 1.5

Catalogue number: FG-SSMAG1.5

Magnetic Stands for the purification with magnetic beads

Magnetic Beads have been used for a long time to isolate nucleic acids as well as recombinant proteins. The purification was however problematic, often with carry over when the pellet was disturbed or with contamination when the supernatant was not completely removed.

The FastGene® MagnaStands solve both issues. By creating a pellet at the side walls rather than at the bottom, the MagnaStands allow with their very strong neodymium magnets the complete removal of the supernatant without touching the pellet.

Additionally, with the MagnaStand 1.5, the vertical position is adjustable allowing the magnets’ place to be fine tuned on the tube according to volume used in the purification.

Neodymium Magnets for faster attraction of magnetic particles

The Neodymium magnets are the strongest permanent magnets in the world. The advantages of using such strong magnets are:

  • Very quick separation of magnetic beads from the solution
  • Stable pellet even when resuspending
  • Complete collection of the magnetic beads for less material loss

Together with the vertical position of the magnets, the magnetic strength gives you the optimal solution for purification of magnetic beads.

  • Separation of magnetic beads.
  • NGS library preparation
  • recombinant protein
Material  Aluminium body
Magnets Neodymium Magnets
Number of Magnets  8 or 96 magnets

MagnaStand 1.5

For large volumes using 1.5 ml tubes. The position of the magnets is adjustable in horizontal as well as vertical axis.

FG-SSMAG1.5 magnetic stand

Perform up to 8 purifications in parallel
Extra large magnets for larger volumes
Adjust the magnet position to the volume of your sample

MagnaStand 0.2

For purifications of ultra low volumes (down to 5 µl) using normal PCR-tubes. The optimal horizontal position can be fine-tuned.

The FastGene® MagnaStand (FG-SSMAG2) from Nippon Genetics was implemented in the diagnostic lab of our institute to isolate genomic DNA from patients, for a SureSelectQXT Target Enrichment (Agilent), before being sequenced using a MiSeq instrument (Illumina). The FastGene® Magna Stand has been used in all steps where AMPure XP beads (BeckmanCoulter) or Dynabeads (Life Technology) were utilized.
The handling of the FastGene® Magna Stands is very easy and the results obtained so far are good. The pellet stays, as desired, at the side of the tube wall, enabling an easy removal of the supernatant. In some cases, the complete removal of the elution was possible. The FastGene® Magna Stand is compact and robust. We are very satisfied with it and would recommend it any time!
Simone Rost, PhD – Institute of Human Genetics – University of Würzburg – Germany

  • Perform up to 8 purifications in parallel
  • Optimized for extra small volumes
  • Adjust the magnet position for closer contact to the tube

FG-SSMAG2 Magnetic stand

MagnaStand 96

  • Perform up to 96 purifications in parallel
  • Compatible with Full-, Semi-, and Non-skirted (with adapter) 96 well plates

Can you give me some Technical Data about the MagnaStand and how is the N-rating of the magnets?

A neodymium magnet (also known as NdFeB, NIB or Neo magnet), the most widely used type of rare-earth magnet, is a permanent magnet made from an alloy of neodymium, iron and boron to form the Nd2Fe14B tetragonal crystalline structure. Developed in 1982 by General Motors and Sumitomo Special Metals, neodymium magnets are the strongest type of permanent magnet commercially available. They have replaced other types of magnets in the many applications in modern products that require strong permanent magnets, such as motors in cordless tools, hard disk drives and magnetic fasteners. Neodymium magnets are graded according to their maximum energy product, which relates to the magnetic flux output per unit volume. Higher values indicate stronger magnets and range from N35 up to N52. Letters following the grade indicate maximum operating temperatures (often the Curie temperature), which range from M (up to 100 C) to EH (200 C).


 

What does the “N rating”, or grade, of the neodymium magnets mean?

The grade, or “N rating” of the magnet refers to the Maximum Energy Product of the material that the magnet is made from. It refers to the maximum strength that the material can be magnetized to. The grade of neodymium magnets is generally measured in units millions of Gauss Oersted (MGOe). A magnet of grade N42 has a Maximum Energy Product of 42 MGOe. Generally speaking, the higher the grade, the stronger the magnet.

We can state that for all magnets the N-rating is “N40”.

on 02/24/2020

Der Magnet ist seit mehr als einem Jahr im Dauerbetrieb und überzeugt durch seine robuste Verarbeitung, Magnetstärke, sowie die verstellbare Magnetschiene. Deshalb haben wir uns auch noch den Magnastand 0,2 zugelegt.

on 02/24/2020

Der Magnet ist seit mehr als einem Jahr im Dauerbetrieb und überzeugt durch seine robuste Verarbeitung, Magnetstärke, sowie die verstellbare Magnetschiene. Deshalb haben wir uns auch noch den Magnastand 0,2 zugelegt.

on 03/20/2019

Wir verwenden sowohl den Magnastand 0,2 als auch den 1,5 für die Library-Präparation in unserer Routinediagnostik. Nach Austesten verschiedener Anbieter und auch eines Eigenbaus sind wir mit diesem Produkt hochzufrieden.

Translation from Nippon Genetics:

We use both the MagnaStand for 0.2 and 1.5 ml for the preparation of cDNA libraries. We are very satisfied with this product. Before using the MagnaStand we test several magnetic stands from other companies and a homemade magnetic stand.

on 03/06/2019

My research involves purifying DNA from a very small sample volume, often in a 96-well format. The first rack I had used a magnet in the form of an O-ring. Unfortunately, it did not work well below 50ul. The shape of the magnet also caused the problem since using it is convenient to support the tip of multichannel pipette on the PCR tubes. The Nippon magnet stands proved to be the perfect solution. Full mobility of the magnet holder makes it possible to adapt it to any volume and model of PCR tubes. In addition, it is possible to purify DNA from different sample volumes, thanks to individually fitted magnets. Another advantage is the possibility of firmly fixing the eppendorf (so that it does not fall out) and setting the entire stand in any position, even upside down. Since the whole stand is made of metal, it is very convenient to elute ssDNA from biotinylated DNA caught on streptavidin by heating the whole to 70°C. And all this made me a happy owner of 3 different Nippon stands so far 🙂

on 03/05/2019

Aufgrund seiner individuellen Einstellmöglichkeiten ist dieser robuste Magnetständer bei uns im Labor bei verschiedenen Applikationen im Einsatz. Alle Anwender sind sehr zufrieden. Klare Empfehlung

Translation by Nippon Genetics:

Due to its individual setting options, this robust magnetic stand is used by us in the laboratory for various applications. All users are very satisfied with it. I can absolutely recommend this product.

on 01/26/2018

„Actually Postdoc using it is truly happy with it, she will recommend to anybody will ask“.

on 01/26/2018

„Wir haben verschiedene magnetic racks für PCR-tubes verglichen und der FastGene® MagnaStand 0.2 hat uns mit seiner Robustheit, Magnetstärke, Justierbarkeit und dem guten Halt der PCR-tubes überzeugt.“

on 01/26/2018

„Der Magnetständer FastGene® MagnaStand 0.2 der Firma Nippon Genetics wird im Diagnostik-Labor unseres Instituts für das SureSelectQXT Target Enrichment (Agilent) von genomischer Patienten-DNA zur Sequenzierung am MiSeq (Illumina) verwendet. Bei folgenden Schritten wird der Magnetständer eingesetzt: während der beiden Aufreinigungsschritte der Library mittels AMPure XP Beads, während der „Capture“-Schritte der hybridisierten DNA mittels Streptavidin-coated Magnetic Beads (Dynabeads von Life Technologie), sowie während der finalen Aufreinigung der amplifizierten Library mittels AMPure XP Beads. Zusammenfassend wird der Magnetständer folglich in jedem Schritt eingesetzt, in dem entweder AMPure Beads oder Dynabeads verwendet werden. Die Dynabeads lassen sich etwas schlechter trennen als die AMPure Beads, aber dieses Problem scheint unabhängig vom verwendeten Magnetständer aufzutreten.“

Translation by Nippon Genetics

“The magnetic stand FastGene® Magna Stand (FG-SSMAG2) from Nippon Genetics was implemented in the diagnostic lab of our institute to isolate genomic DNA from patients, for a SureSelectQXT Target Enrichment (Agilent), before being sequenced using a MiSeq instrument (Illumina). The FastGene® Magna Stand has been used in all steps where AMPure XP beads (BeckmanCoulter) or Dynabeads (Life Technology) were utilized. The handling of the FastGene® Magna Stands is very easy and the results obtained so far are good. The pellet stays, as desired, at the side of the tube wall, enabling an easy removal of the supernatant. In some cases, the complete removal of the elution was possible. The FastGene® Magna Stand is compact and robust. We are very satisfied with it and would recommend it any time!”

on 10/24/2017

“More and more applications are performed using magnetic beads, so that a magnetic stand is required which can handle very small volumes. Especially in the genomics research field, where massively parallel sequencing is performed, it is essential for an ideal magnetic stand that is used for the purification of DNA and RNA, to be compatible with a variety of commercially available PCR tubes and plates. The available magnetic stands could not capture the magnetic beads firmly or provide an easy handling for the researcher. The handling of the FastGene® MagnaStands is easy, without any loss of magnetic beads during purification even when very few µl of liquid volume were used. Hence, it is the solution for the well-known and common problem of sample loss due to magnetic beads being aspirated by the pipette during a purification using a small sample volume. The FastGene® MagnaStand 0.2 and the FastGene® MagnaStand 1.5 can be used for the purification of small number of samples. The FastGene® MagnaStand 96 is useful for the purification of a large number of samples, e.g. for single-cell genomic research. All FastGene® MagnaStands are the best tool to easily purify magnetic beads from small volumes, since the magnetic beads are firmly held in one position of the tube wall.”

Translation by Nippon Genetics

„Immer mehr Applikationen werden mit Hilfe von Magnetic Beads durchgeführt, so dass Magnetische Stands benötigt werden, die auch sehr kleine Volumen händeln können. Besonders im Bereich der genomischen Forschung, in der äußerst viele parallele Sequenzierungen durchgeführt werden, sind für die DNA und RNA Aufreinigung benutzte magnetische Stands essentiell, die kompatibel mit diversen kommerziell erhältlichen PCR Reaktionsgefäßen und Platten sind. Die bisher erhältlichen Magnetic Stands konnten die Magnetic Beads nicht gänzlich erfassen oder waren nur schwer bedienbar. Die Handhabung von FastGene® MagnaStands ist einfach und Magnetic Beads gehen während der Aufreinigung nicht einmal dann verloren, wenn nur wenige µl Flüssikeit verwendet werden. Somit sind sie die Lösung für das altbekannte, häufig auftretende Problem des Probeverlustes durch das Einsaugen der Magnetic Beads mit der Pipette bei der Verwendung geringer Probevolumen. Das FastGene® MagnaStand 0.2 und das FastGene® MagnaStand 1.5 kann für die Aufreinigung einer kleinen Anzahl von Proben verwendet werden. Das FastGene® MagnaStand 96 ist nützlich für die Aufreinigung einer großen Anzahl von Proben, z.B. für eine Single cell Genomsequenzierung. Alle FastGene® MagnaStands sind die besten Arbeitshilfen zum Reinigen von Magnetic Beads, die in geringen Volumen vorliegen, da die Magnetic Beads fest in einer Position an der Wand des Raktionsgefäßes gehalten werden.

Dr. Yohei Sasagawa ist ein Forscher der in Zusammenarbeit mit Nippon Genetics die FastGene® MagnaStands für seine NGS Library Präparation entwickelt hat.
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