Magnetic Beads have been used for a long time to isolate nucleic acids as well as recombinant proteins. The purification was however problematic, often with carry over when the pellet was disturbed or with contamination when the supernatant was not completely removed.
The FastGene® MagnaStands solve both issues. By creating a pellet at the side walls rather than at the bottom, the MagnaStands allow with their very strong neodymium magnets the complete removal of the supernatant without touching the pellet.
Additionally, with the MagnaStand 1.5, the vertical position is adjustable allowing the magnets’ place to be fine tuned on the tube according to volume used in the purification.
Neodymium Magnets for faster attraction of magnetic particles
The Neodymium magnets are the strongest permanent magnets in the world. The advantages of using such strong magnets are:
Together with the vertical position of the magnets, the magnetic strength gives you the optimal solution for purification of magnetic beads.
|Number of Magnets||8 or 96 magnets|
For large volumes using 1.5 ml tubes. The position of the magnets is adjustable in horizontal as well as vertical axis.
Perform up to 8 purifications in parallel
Extra large magnets for larger volumes
Adjust the magnet position to the volume of your sample
For purifications of ultra low volumes (down to 5 µl) using normal PCR-tubes. The optimal horizontal position can be fine-tuned.
The FastGene® MagnaStand (FG-SSMAG2) from Nippon Genetics was implemented in the diagnostic lab of our institute to isolate genomic DNA from patients, for a SureSelectQXT Target Enrichment (Agilent), before being sequenced using a MiSeq instrument (Illumina). The FastGene® Magna Stand has been used in all steps where AMPure XP beads (BeckmanCoulter) or Dynabeads (Life Technology) were utilized.
The handling of the FastGene® Magna Stands is very easy and the results obtained so far are good. The pellet stays, as desired, at the side of the tube wall, enabling an easy removal of the supernatant. In some cases, the complete removal of the elution was possible. The FastGene® Magna Stand is compact and robust. We are very satisfied with it and would recommend it any time!
Simone Rost, PhD – Institute of Human Genetics – University of Würzburg – Germany
A neodymium magnet (also known as NdFeB, NIB or Neo magnet), the most widely used type of rare-earth magnet, is a permanent magnet made from an alloy of neodymium, iron and boron to form the Nd2Fe14B tetragonal crystalline structure. Developed in 1982 by General Motors and Sumitomo Special Metals, neodymium magnets are the strongest type of permanent magnet commercially available. They have replaced other types of magnets in the many applications in modern products that require strong permanent magnets, such as motors in cordless tools, hard disk drives and magnetic fasteners. Neodymium magnets are graded according to their maximum energy product, which relates to the magnetic flux output per unit volume. Higher values indicate stronger magnets and range from N35 up to N52. Letters following the grade indicate maximum operating temperatures (often the Curie temperature), which range from M (up to 100 C) to EH (200 C).
The grade, or “N rating” of the magnet refers to the Maximum Energy Product of the material that the magnet is made from. It refers to the maximum strength that the material can be magnetized to. The grade of neodymium magnets is generally measured in units millions of Gauss Oersted (MGOe). A magnet of grade N42 has a Maximum Energy Product of 42 MGOe. Generally speaking, the higher the grade, the stronger the magnet.
We can state that for all magnets the N-rating is “N40”.