100 preparations for the isolation of low-copy and high-copy plasmid DNA. Inclusive Ready-to-use LB medium capsules.
are designed for rapid small scale isolation and purification of high copy and low copy plasmid DNA. The ready-to-use plasmid DNA is of high quality in low-salt Tris buffer and suitable for typical downstream applications: Cloning, sequencing, PCR, transformation and restriction analysis.
|Parameter||High-copy Plasmid||Low-copy Plasmid|
|Max. sample volume||1-5 ml ON-culture||5-10 ml ON-culture|
|Typical yield||< 25 µg||< 25 µg|
|Elution volume||50 µl||50 µl|
|Binding capacity||40 µg||40 µg|
|Size of vector||< 15 kb||< 15 kb|
|Prep time||26 min / 12 preps||26 min / 12 preps|
|Format||Spin column||Spin column|
The gel demonstrates that FastGene® Plasmid Mini Kits yield equal amount of plasmid DNA compared to other supplier with even more time consuming protocols. The preparation with the FastGene® Plasmid Mini Kit was performed by using the Fast Protocol.
Fig. 1: pBluescript plasmid DNA was isolated from a 1,4 ml E.coli culture (DH5α in LB medium) according to the recommended
procedures of different kits and eluted in 50 µl elution buffer. 2 µl of each eluate were loaded on a 0,7% TAE agarose gel.
Fig.2: Typical fluorescent capillary sequencing result from pBluescript plasmid DNA purified using the FastGene® Plasmid Mini Kit. Cycle sequencing reaction was performed on an ABI3100 sequenzer. 1 µl of purified plasmid DNA was used as a template. Dye terminators were removed by FastGene® Dye Terminator Removal Kit
We would not recommend to use the Plasmid Kit with the Qiacube. The problem is the fitting of the tube. It must fit perfectly, otherwise it could lead to leaking of the tubes.