Translated by Nippon Genetics:
"It is easy to use and leads to very nice results, combined with a very good price."
The FastGene® Scriptase Basic is an enhanced version of the Murine Leukemia Virus (MuLV) reverse transcriptase. Hence, like the wildtype, it has the ability to synthesize a cDNA strand, a reduced RNase H activity and processitivity. The robustness however was greatly increased. It is the perfect enzyme for large RNA quantities and easy templates.
The special buffer formulation permits a high RNA concentration. Other reverse transcriptases are not able to process such large quantities.
The FastGene® Scriptase Basic was designed for large RNA quantities, typically used in an endpoint RT-PCR. Nonetheless, it is also able to process lower RNA concentrations.
The FastGene® Scriptase Basic is available as enzyme only, containing the enzyme, buffer and dNTPs as well as a
cDNA synthesis kit which contains the components of the enzyme plus Oligo dTs, random hexamers and RNase inhibitor. We have also chosen a 100 reactions kit size so that you have enough for a whole 96-well plate.
(A) Competitor N (B) FastGene® Scriptase Basic
RNA (ng) 2 1 0.5 0.1 0.01 2 1 0.5 0.1 0.01
Fig. 1: Higher sensitivity of the FastGene® Scriptase Basic.
Compared to a wildtype MuLV (A) the FastGene® Scriptase Basic (B) is an optimized MuLV-Reverse transcriptase which has a higher sensitivity, being able to produce a template from RNA concentrations as low as 0.1 ng.