FastGene Taq HotStart DNA Polymerase (1000 U)

Catalogue number: LS25

FastGene® DNA HotStart Polymerase is combined with a proprietary antibody that inactivates the enzyme until the first denaturation step. This eliminates spurious amplification products resulting from non-specific priming events during reaction setup and initiation, and increases overall reaction efficiency and sensitivity.

The reaction buffer does not include MgCl2 but the kit includes a separate vial with Magnesiumchloride.

Fig. 1: Reactions (25 µl) were set up according to manufacturer’s instructions, with 20 ng of hgDNA as template, and 0.5 µM of each primer. DMSO was included at a final concentration of 5%. PCR was performed for a total of 35 cycles.

Fig. 2: Reactions (25 µl) were set up according to manufacturer’s instructions, with 25 ng of hgDNA as template,
and 0.5 µM of each primer. Qiagen Q-solution was included at a final concentration of 1X. PCR was performed for a total of 35 cycles.

Cat. No. Product Content
LS23 FastGene® HotStart TAQ DNA polymerase 100 Units
LS24 FastGene® HotStart TAQ DNA polymerase 250 Units
LS25 FastGene® HotStart TAQ DNA polymerase 1000 Units

 

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