# Qsep Bio-Fragment Analyzer --- url: https://www.nippongenetics.eu/en/products/capillary-gel-electrophoresis/qsep-fragment-analyzer/qsep-bio-fragment-analyzer/ language: en type: product modified: 2026-04-09T09:05:41+02:00 --- ## Capillary gel electrophoresis for DNA, RNA and proteins ## Product Details - **SKU**: C400100, C100200, C100001, C100001-P - **Stock status**: instock - **Categories**: Capillary Gel Electrophoresis, Qsep Fragment Analyzer ## Attributes - **Sample amount**: 1-8 samples (1 channel), < 96 samples (1 channel), < 96 samples (4 channels) - Fully automated fragment analyzer for DNA, RNA and protein analysis - Easiest handling thanks to ready-to-use, [exchangeable cartridges](https://www.nippongenetics.eu/en/product-category/capillary-gel-electrophoresis/qsep-cartridges/) with integrated capillaries, fluorophores and gel matrices (patented technology [(link)](https://www.nippongenetics.eu/app/uploads/2020/06/BiOptic-european-patent-specification-EP11770927NWB1.pdf)) - Perfect results and reproducible results without extensive training - 4 models are available differing in number of samples that can be performed per run: - Qsep1 series: - Qsep1: For 1 to 8 samples (1 channel) - Qsep1-Plus: For 1 to 16 samples (1 channel) - Qsep100 2.0: For up to 96 samples (1 channel) - Qsep400: For up to 96 samples (4 channels) [PRODUCT INQUIRY](https://www.nippongenetics.eu/en/product-inquiry-qsep-fragment-analyzer/) ### Details ##### Technology The Qsep Bio-Fragment Analyzer replaces old-fashioned, labor-intensive gel analysis of DNA, RNA and proteins. The single-channel capillary electrophoresis (CGE) system automates sensitive, high-resolution analysis of 1 to 96 samples, in 2-7 minutes per run. Ready-to-run gel [cartridges](https://www.nippongenetics.eu/en/product-category/capillary-gel-electrophoresis/qsep-cartridges/) allow up to 200 samples to be analyzed with a minimum of hands-on interaction, reducing manual handling errors and eliminating the need for gel preparation. An intuitive Q-Analyzer software ensures convenient analysis and documentation of data. The Qsep Bioanalyzers provide accurate results in three simple steps: 1. Insert the disposable pen-shaped gel cartridge into the instrument. 2. Place the samples in the sample tray. 3. Choose the appropriate method and run. In just a few minutes, users can get reliable results in diverse formats (PDF, Word, Excel), including peak and gel charts. ![Qsep performance in three steps](https://www.nippongenetics.eu/app/uploads/2020/06/Qsep-Durchfuehrung-Schritte-en.png) ##### **Availability** The Qsep Fragment Analyzers are available in Germany, Austria, Belgium, Denmark, Finnland, Greece, Croatia and Serbia. For other countries, [please contact us](#section-inquiry_form). ##### Qsep instruments The four Qsep Fragment Analyzer models differ in the number of samples that can be performed per run. Handling, technology as well as the type and quality of the feasible applications are identical. **Qsep1: For 1 to 8 samples (1 channel)** The Qsep1 instrument is comprised of a capillary gel electrophoresis (CGE) core integrated with a high sensitivity LED-induced fluorescence (LEDIF) detector and 1 to 8 well auto-sampler. The replaceable gel-cartridge automatically regenerates the capillary gel between runs and can be used for up to 200 sample injections providing robust and superior results for all kinds of molecular genetic analysis applications. Qsep1 with variety of replaceable gel-cartridge kit is specifically designed for DNA, RNA and Protein analysis providing reproducible and accurate test results in matter of seconds. Qsep1 comes with Q-Analyzer software which has a simple-to-use User Interface (UI) that delivers consistent and high-precision data analysis and data export functions helping users to turn molecular biology into “Moving” biology. ![](https://www.nippongenetics.eu/app/uploads/2020/06/qsep1-1-300x300.png) **Qsep1-Plus: For 1 to 16 samples (1 channel)** Qsep1-Plus is ideal for small laboratories with low throughput (1~16 samples) needs (details, see Qsep1). ![](https://www.nippongenetics.eu/app/uploads/bio-fragment-analyzer-qsep1-plus.png) **Qsep100 2.0: For up to 96 samples (1 channel)** Qsep100 2.0 is the updated version of the Qsep100 (C100100). It is is a small, simple and user friendly bench top capillary gel electrophoresis (CGE) system integrated with a high sensitivity LED-induced fluorescence (LEDIF) detector with an easily-replaceable gel cartridge and 96-well auto-sampler. The 150 mm long capillary mounted into the cartridge contains a gel matrix that is replenished from the top reservoir of the cartridge automatically between sample runs. The reservoir provides sufficient gel for up to 200 sample injections providing robust and superior results for all kinds of molecular genetic analysis applications. Qsep100 2.0 with variety of replaceable gel-cartridge kit is specifically designed for DNA, RNA and Protein analysis providing reproducible and accurate test results in matter of seconds. Qsep100 2.0 comes with Q-Analyzer software which has a simple-to-use User Interface (UI) that delivers consistent and high-precision data analysis and data export functions helping users in their daily molecular biology experiments. The updated version 2.0 not only delivers reliable performance but also introduces several important improvements: - Modernized design - Reduced sample input volume: 0.2 mL PCR tube now requires only 10 μL, compared to the previous 20 μL. - A wider sample door and built-in lighting inside the unit, allowing customers to clearly view the tray and sample status during operation. ![Qsep100 2.0 Bio-Fragment Analyzer | Front view](https://www.nippongenetics.eu/app/uploads/qsep100-2-0-front-view-300x300.png) **Qsep 400: For up to 96 samples (4 channels)** Qsep400 has the highest throughput in the Qsep family. The 4-channel instrument is designed as a 4-capillaries gel electrophoresis (CGE) system, integrated with a high sensitivity LED-induced fluorescence (LEDIF) detector with an easily-replaceable 4-channel gel-cartridge and 96-well auto-sampler, which can analyze / detect four samples at the same time. Qsep400 has been designed for the larger research and clinical laboratories, who have large sample volumes. The high-throughput system provides high-speed analysis, superior results for all kinds of molecular genetic analysis applications. Qsep400 with variety of replaceable gel-cartridge kit is specifically designed for DNA, RNA and Protein analysis providing reproducible and accurate test results in matter of seconds. Qsep400 comes with Q-Analyzer software which has a simple-to-use User Interface (UI) that delivers consistent and high-precision data analysis and data export functions helping users in their daily molecular biology experiments. ![](https://www.nippongenetics.eu/app/uploads/2020/07/qsep400wp-300x300.png) ### Specifications ##### Specifications ##### Qsep1 ##### Qsep1-Plus ##### Qsep100 2.0 ##### Qsep 400 ##### ![Qsep1 Bio-Fragment Analyzer | Front view](https://www.nippongenetics.eu/app/uploads/qsep1-front-view-300x300.png) ##### ![Qsep1-Plus Bio-Fragment Analyzer | Front view](https://www.nippongenetics.eu/app/uploads/qsep1-plus-front-view-150x150.png) ##### ![Qsep100 2.0 Bio-Fragment Analyzer | Front view](https://www.nippongenetics.eu/app/uploads/qsep100-2-0-front-view-150x150.png) ##### ![Qsep400 Bio-Fragment Analyzer | Front view](https://www.nippongenetics.eu/app/uploads/qsep400-front-view-150x150.png) Cat. No.C100001C100001-PC100200C400100LED Light Source525 nm525 nm525 nm525 nmFluorescence Detection590 nm590 nm590 nm590 nmSeparation Voltage1-8 KV1-8 KV1-15 KV1-15 KVConnectionWiFi and CableWiFi and CableUSBUSBPour SourceAC 100-240VAC 100-240VAC 100-240VAC 100-240VMaximum Power30W30W30W60WDimension240 mm x 210 mm x 300 mm240 mm x 210 mm x 300 mm410 mm x 300 mm x 430mm540 mm x 400 mm x 360mmWeight5.5 kg5.5 kg16.5 kg26 kg##### **Highlights** System Type1 Channel Portable System1 Channel Portable System1 Channel System4 Channel SystemAutomated Sampling1-8 samples1-16 samples1-96 samples1-96 samplesRapid Analysis2-7 min/sample2-7 min/sample2-7 min/sample2-7 min/ 4 samplesResolution1-4 bp (between 100-500 bp)1-4 bp (between 100-500 bp)1-4 bp (between 100-500 bp)1-4 bp (between 100-500 bp)Detection Sensitivity5 pg/uL (2 pg/µL)5 pg/uL (2 pg/µL)- 5 pg/uL (2 pg/µL) - 1 pg/µL (if diluted in ddH2O) 5 pg/uL (2 pg/µL)Minimum Sample Volume2 µL (Micro-Vial)2 µL (Micro-Vial)- 2 µL (Micro-Vial) - 10 µL (0.1 and 0.2 mL tube) 2 µL (Micro-Vial)SoftwareQ-AnalyzerQ-AnalyzerQ-AnalyzerQ-AnalyzerL.O.D.: 2pg/ul (if diluted in water) ### Applications **Standard Cartridge (S2)****High Resolution Cartridge (S1)****Kilobase Cartridge (S3)****Fast Cartridge (F3)** Only suitable for Qsep100**High Sensitivity Cartridge (N1)****RNA Cartridge (R1)****Protein Cartridge (P2)****High sensitivity Kilobase Cartridge (N3)****High sensitivity RNA Cartridge (NR1)**PCR product screeningxxxRestriction Fragment Length Polymorphism (RFLP)xxxPlasmid purification & Vector cloning analysisxxNext Generation Sequencing (NGS) QCxGenomic DNA analysisxxCell free DNA detectionxRNA AnalysisxxLow concentration samplexxxCRISPR QC & AnalysisxxxxLarge size analysis (>50kb)xxReplace SDS-PAGExIgG Purity Testx[Cartridges](https://www.nippongenetics.eu/app/uploads/2020/06/cartridges.jpg) ### DNA **Cartridge name / Specfication****Standard Cartridge Kit(S2)****High Resolution Cartridge Kit (S1)****Fast Cartridge Kit (F3)** Only suitable for Qsep100**High Sensitivity Cartridge Kit (N1)****Kilo Base Cartridge Kit (S3)****High Sensitivity Kilo Base Cartridge Kit (N3)****Standard Quantitative Cartridge Kit (S2)****High Resolution Quantitative Cartridge Kit (S1)****High Sensitivity Quantitative Cartridge Kit (N1)****1-channel | 1 cartridge** [C105101](https://www.nippongenetics.eu/en/products/capillary-gel-electrophoresis/qsep-cartridges/cartridges-qsep100/) (1 pcs)[C105102](https://www.nippongenetics.eu/en/products/capillary-gel-electrophoresis/qsep-cartridges/cartridges-qsep100/) (1 pcs)[C105103](https://www.nippongenetics.eu/en/products/capillary-gel-electrophoresis/qsep-cartridges/cartridges-qsep100/) (1 pcs)[C105105](https://www.nippongenetics.eu/en/products/capillary-gel-electrophoresis/qsep-cartridges/cartridges-qsep100/) (1 pc)[C105106](https://www.nippongenetics.eu/en/products/capillary-gel-electrophoresis/qsep-cartridges/cartridges-qsep100/) (1 pcs)[C105153](https://www.nippongenetics.eu/en/products/capillary-gel-electrophoresis/qsep-cartridges/cartridges-qsep100/) (1 pc)**1-channel | 2 cartridges**[C105201](https://www.nippongenetics.eu/en/products/capillary-gel-electrophoresis/qsep-cartridges/cartridges-qsep100/) (2 pcs)[C105202](https://www.nippongenetics.eu/en/products/capillary-gel-electrophoresis/qsep-cartridges/cartridges-qsep100/) (2 pcs)[C105203](https://www.nippongenetics.eu/en/products/capillary-gel-electrophoresis/qsep-cartridges/cartridges-qsep100/) (2 pcs)[C105205](https://www.nippongenetics.eu/en/products/capillary-gel-electrophoresis/qsep-cartridges/cartridges-qsep100/) (2 pcs)[C105206](https://www.nippongenetics.eu/en/products/capillary-gel-electrophoresis/qsep-cartridges/cartridges-qsep100/) (2 pcs)[C105253](https://www.nippongenetics.eu/en/products/capillary-gel-electrophoresis/qsep-cartridges/cartridges-qsep100/) (2 pcs)[C105201-Q](https://www.nippongenetics.eu/en/products/capillary-gel-electrophoresis/qsep-cartridges/cartridges-qsep100/https://www.nippongenetics.eu/en/products/capillary-gel-electrophoresis/qsep-cartridges/cartridges-qsep100/) (2 pcs)[C105202-Q](https://www.nippongenetics.eu/en/products/capillary-gel-electrophoresis/qsep-cartridges/cartridges-qsep100/) (2 pcs)[C105205-Q](https://www.nippongenetics.eu/en/products/capillary-gel-electrophoresis/qsep-cartridges/cartridges-qsep100/) (2 pcs)**1-channel | 8 cartridges**[C105801](https://www.nippongenetics.eu/en/products/capillary-gel-electrophoresis/qsep-cartridges/cartridges-qsep100/) (8 pcs)[C105802](https://www.nippongenetics.eu/en/products/capillary-gel-electrophoresis/qsep-cartridges/cartridges-qsep100/) (8 pcs)[C105803](https://www.nippongenetics.eu/en/products/capillary-gel-electrophoresis/qsep-cartridges/cartridges-qsep100/) (8 pcs)[C105806](https://www.nippongenetics.eu/en/products/capillary-gel-electrophoresis/qsep-cartridges/cartridges-qsep100/) (8 pcs)**4-channels | 1 cartridge**[C405101](https://www.nippongenetics.eu/en/products/capillary-gel-electrophoresis/qsep-cartridges/cartridges-for-qsep-biofragment-analyzer-qsep400/) (1 pc)[C405102](https://www.nippongenetics.eu/en/products/capillary-gel-electrophoresis/qsep-cartridges/cartridges-for-qsep-biofragment-analyzer-qsep400/) (1 pc)[C405103](https://www.nippongenetics.eu/en/products/capillary-gel-electrophoresis/qsep-cartridges/cartridges-for-qsep-biofragment-analyzer-qsep400/) (1 pc)[C405105](https://www.nippongenetics.eu/en/products/capillary-gel-electrophoresis/qsep-cartridges/cartridges-for-qsep-biofragment-analyzer-qsep400/) (1 pc)[C405106](https://www.nippongenetics.eu/en/products/capillary-gel-electrophoresis/qsep-cartridges/cartridges-for-qsep-biofragment-analyzer-qsep400/) (1 pc)[C405101-Q](https://www.nippongenetics.eu/en/products/capillary-gel-electrophoresis/qsep-cartridges/cartridges-for-qsep-biofragment-analyzer-qsep400/) (1 pc)[C405102-Q](https://www.nippongenetics.eu/en/products/capillary-gel-electrophoresis/qsep-cartridges/cartridges-for-qsep-biofragment-analyzer-qsep400/) (1 pc)**Sample size range**10-5000 bp10-5000 bp10-5000 bp10-5000 bp10-50000 bp10-50000 bp10-1500 bp10-1500 bp10-5000 bp**L.O.D.**0.1 ng/uL\*0.1 ng/uL\*0.1 ng/uL\*5 pg/uL\*0.1 ng/uL\*5 pg/uL\*0.1 ng/uL\*0.1 ng/uL\*5 pg/uL\***Best Resolution**4-10 bp1-4 bp>50 bp>10 bp10-50 bp10-50 bp4-10 bp1-4 bp>10 bp**Analysis time (per sample)**2-3 mins3-5 mins1-2 mins2-3 mins5-21 mins9-25 mins2-3 mins3-5 mins5-21 mins**Sample number (per cartridge)**200 runs200 runs300 runs100 runs200 runs100 runs200 runs200 runs200 runs**Sample consumption (per run)**<0.1 µL<0.1 µL<0.1 µL<0.1 µL<0.1 µL<0.1 µL<0.1 µL<0.1 µL<0.1 µL**Recommended sample volume**0.1 mL tube: 10 µL0.1 mL tube: 10 µL0.1 mL tube: 10 µL0.1 mL tube: 10 µL0.1 mL tube: 10 µL0.1 mL tube: 10 µL0.1 mL tube: 10 µL0.1 mL tube: 10 µL0.1 mL tube: 10 µL0.2 mL tube: 20 µL0.2 mL tube: 20 µL0.2 mL tube: 20 µL0.2 mL tube: 20 µL0.2 mL tube: 20 µL0.2 mL tube: 20 µL0.2 mL tube: 20 µL0.2 mL tube: 20 µL0.2 mL tube: 20 µLMicro vial: 2 µL (C104250)Micro vial: 2 µL (C104250)Micro vial: 2 µL (C104250)Micro vial: 2 µL (C104250)Micro vial: 2 µL (C104250)Micro vial: 2 µL (C104250)**Shelf life**6 months6 months4 months3 months6 months3 months6 months6 months3 months\* Determined by the DNA ladder 15-622 (DNA size marker C109200) L.O.D.: 2pg/µL (if diluted in water) ### RNA and Proteins **Cartridge name / Specfication****RNA Cartridge (R1)****High Sensitivity RNA Cartridge (NR1)****Protein Cartridge (SDS) (P2)****1-channel | 1 cartridge**[C105110](https://www.nippongenetics.eu/en/products/capillary-gel-electrophoresis/qsep-cartridges/cartridges-qsep100/) (1 pc)[C105111](https://www.nippongenetics.eu/en/products/capillary-gel-electrophoresis/qsep-cartridges/cartridges-qsep100/) (1 pc)[C105121](https://www.nippongenetics.eu/en/products/capillary-gel-electrophoresis/qsep-cartridges/cartridges-qsep100/) (1 pc)**1-channel | 2 cartridges**[C105210](https://www.nippongenetics.eu/en/products/capillary-gel-electrophoresis/qsep-cartridges/cartridges-qsep100/) (2 pcs)[C105211](https://www.nippongenetics.eu/en/products/capillary-gel-electrophoresis/qsep-cartridges/cartridges-qsep100/) (2 pcs)[C105221](https://www.nippongenetics.eu/en/products/capillary-gel-electrophoresis/qsep-cartridges/cartridges-qsep100/) (2 pcs)**1-channel | 8 cartridges**[C105810](https://www.nippongenetics.eu/en/products/capillary-gel-electrophoresis/qsep-cartridges/cartridges-qsep100/) (8 pcs)**4-channels | 1 cartridge**[C405110](https://www.nippongenetics.eu/en/products/capillary-gel-electrophoresis/qsep-cartridges/cartridges-for-qsep-biofragment-analyzer-qsep400/) (1 pc)[C405121](https://www.nippongenetics.eu/en/products/capillary-gel-electrophoresis/qsep-cartridges/cartridges-for-qsep-biofragment-analyzer-qsep400/) (1 pc)**Sample size range**N/AN/A14-150 kDa**L.O.D.**5 ng/µL1 ng/µL0.5 ng/µL (BSA)**Best Resolution**N/AN/AN/A**Analysis time (per sample)**5-10 mins5-10 mins6 mins (BSA)10 mins (IgG)**Sample number (per cartridge)**100 runs100 runs100 runs**Sample consumption (per run)**<0.1 µL<0.1 µL<0.1 µL**Recommended sample volume**0.1 mL tube: 10 µL0.1 mL tube: 10 µL0.1 mL tube: 10 µL0.2 mL tube: 20 µL0.2 mL tube: 20 µL0.2 mL tube: 20 µLMicro vial: 1 µLMicro vial: 1 µL(C104250)(C104250)**Shelf life**3 months3 months3 monthsL.O.D.: 2pg/µL (if diluted in water) ### Downloads & Service ##### Service for customers ##### [REgister your instrument](https://www.nippongenetics.eu/en/instrument-registration/) ###### Activate our exclusive service now & enjoy your benefits - **Priority access** to latest software updates - **Fast line** to our expert service team - **Sneak peak** at new features and accessories [More information](https://www.nippongenetics.eu/en/instrument-registration/) ##### Manuals [Manual | Qsep1, Qsep1-Plus Bio Fragment Analyzer](https://www.nippongenetics.eu/app/uploads/manual-qsep1-qsep1plus-bio-fragment-analyzer.pdf) [Manual | Qsep400 Bio Fragment Analyzer](https://www.nippongenetics.eu/app/uploads/manual-qsep400-bio-fragment-analyzer.pdf) [F0045\_Qsep Series Operation Manaual-Software-ENG-A](https://www.nippongenetics.eu/app/uploads/2020/06/F0045_Qsep-Series-Operation-Manaual-Software-ENG-A.pdf) [F0042-Qsep1 Quick start installation CHT&ENG](https://www.nippongenetics.eu/app/uploads/2020/06/F0042-Qsep1-Quick-start-installation-CHTENG.pdf) [F0016-Qsep1-Quick start operation-ENG-C-20190903](https://www.nippongenetics.eu/app/uploads/2020/06/F0016-Qsep1-Quick-start-operation-ENG-C-20190903.pdf) [F0002-Software Installation Quick Start -ENG-E](https://www.nippongenetics.eu/app/uploads/2020/06/F0002-Software-Installation-Quick-Start-ENG-E.pdf) [F0006-Q-Viewer and Q-Editor Quick Start -ENG-D](https://www.nippongenetics.eu/app/uploads/2020/06/F0006-Q-Viewer-and-Q-Editor-Quick-Start-ENG-D.pdf) ##### Flyer [Flyer Qsep Fragment Analyzer](https://www.nippongenetics.eu/app/uploads/Flyer-Qsep-Fragment-Analyzer-EN.pdf) [Flyer Qsep1 Biofragment Analyzer](https://www.nippongenetics.eu/app/uploads/2020/06/flyer-qsep1-biofragment-analyzer.pdf) [Flyer Qsep100 2.0 Biofragment Analyzer](https://www.nippongenetics.eu/app/uploads/flyer-qsep100-2-0-biofragment-analyzer-bioptics.pdf) ##### Patent [Patent BiOptic European Patent Specification](https://www.nippongenetics.eu/app/uploads/2020/06/BiOptic-european-patent-specification-EP11770927NWB1.pdf) ##### Publications [Qsep Bio-Fragment Analyzer: NGS Library Validation Using Automated Capillary Gel Electrophoresis](https://news.nippongenetics.eu/en/qsep-bio-fragment-analyzer-ngs-library-validation-using-automated-capillary-gel-electrophoresis) ### Product Videos ##### NEW video: Qsep Bio-Fragment Analyzer Series Operating procedure | Different devices for different requirements Um das YouTube-Video zu sehen, müssen Sie Cookies akzeptieren. Cookie-Einstellungen ![](https://www.nippongenetics.eu/app/uploads/video-qsep-series-introduction-1024x577.jpg) ![]() ##### Qsep 100 Bio-Fragment Analyzer Applications | Cartridges | Handling and procedure Um das YouTube-Video zu sehen, müssen Sie Cookies akzeptieren. Cookie-Einstellungen ![](https://www.nippongenetics.eu/app/uploads/video-qsep-100-1024x576.jpg) ![]() ### Rated **5** out of 5 based on 1 customer rating 1 review for Qsep Bio-Fragment Analyzer 1. ![](https://secure.gravatar.com/avatar/230a45052f67d8458f3b1b5502362df75e546802c2f30e7a3a0a4c70682d0cf0?s=60&d=mm&r=g)Rated **5** out of 5 **Gotsmann, Eukaryontengenetik, Technische Universität Kaiserslautern** – 06.01.2022 Das Gerät ist absolut Spitze! Auflösung und Sensitivität von dem Gerät sind wirklich toll. Wir haben den Qsep mittlerweile fest in unsere workflows für RNA-Seq und Ribo-Seq Experimente integriert und ich habe bereits einige NGS libraries damit analysiert. Bisher lief immer alles problemlos und wir sind froh, dass wir unsere libraries endlich auch in-house analysieren können, bevor wir sie zum Sequenzieren verschicken. Vor allem die Messung in den micro-vials ist sehr bequem, gerade wenn die Ausbeute bei der library prep eher gering war. Translation by NIPPON Genetics EUROPE: The device is absolutely great! The resolution and sensitivity of the device are really great. We have now firmly integrated the Qsep into our workflows for RNA-Seq and Ribo-Seq experiments and I have already used it to analyze several NGS libraries. So far, everything has always run smoothly and we are happy that we can finally analyze our libraries in-house before we send them off for sequencing. Above all, the measurement in the micro-vials is very convenient, especially when the yield from library prep was rather low. [Verified User](https://www.nippongenetics.eu/en/authenticity-of-reviews/) Only logged in customers may leave a review.