StemFit Basic04 Complete Type

Stem cell culture media

The complete and convenient solution

  • No animal- and human-derived components
  • Manufactured under strictly controlled conditions
  • Media for embryonic stem cells and iPS cells
  • bFGF included – no additional supplement needed
  • Save more than 50% of the media during cell cultivation
  • Reproducible and consistent growth rates
  • Higher proliferation rate
  • Available in Europe
  • High cloning performance
  • Recommended for single cell passaging
Price on request


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Cat. No. Basic04CT

Details

1. Animal-Origin Free

Minimize risk of virus contamination and lot-to-lot variation with animal-origin free formula.

2. Weekend-Free Feeding

The maintenance of stem cells is very complicated and labor intense and the feeding step during the weekends is very tedious. StemFit® allows a feeding-free weekend, by using the recommended weekly workflow shown below.

 

3. Single-Cell Expansion and compatible with various matrices

Experience superior expansion with single-cell passaging and consistent performance with various matrices.

hiPSCs (1210B2) were cultured with StemFit Basic04 on respective cell adhesion substrates. The graph shows accumulated fold expansion.

4. Less media volume needed

Due to the high-quality components and the ideal concentration of nutrients, the required volume of StemFit® is lower than of conventional media. Additionally, reduced feeding steps during the week, creates a total volume difference between StemFit® and conventional media of more than 50%

Culture vessel: 6-well plate | Culture period: 1 week

5. Recommended by the nobel prize winner Shin’ya Yamanaka

StemFit® is recommended by leading scientist. The lab of Shin’ya Yamanake tested StemFit® and obtain excellent results on the differentiation of various cell types via single cell cloning.

“StemFit®, a newly developed Xf-medium, was the best medium for hESC and hiPSC culture with rLN511E8.” Download here the paper

6. One Bottle Composition

Achieve sterilization by simplifying the procedure with ready to use packaging.

7. Consistent gene expression profile

The cultivation of stem-cells is very stressful for the cells. Every passage and growth period could therefore introduce unwanted changes in the genome expression profile. Hence, the CGI Catapult Institute in London investigated the genomic profile of StemFit® after 1 passage, 3 and 5 passages and compared it to 4 commercially available media. The most consistent gene expression was obtained using StemFit® (labeled as AK03N).

9. Less production of lactate

The production of lactate is the result of hypoxic stress. The consequences can be changes in the genome expression profile or lead to unwanted differentiation of the stem cells. The CGI Catapult Institute in London showed that there is considerably less lactate production when the cells are grown in StemFit®.

 

StemFit® Media (code name AK03N) caused a considerably lower accumulation of lactate when compared to 4 other commercially available Stem cell media.

10. High cloning performance

StemFit® Basic02/03 shows a 4-5 higher cloning performance compared to other commercially available media.

The cloning performance of StemFit® Basic04 is about 30% lower than StemFit® Basic02/03, but it is still higher than other commercially available media.

Cloning supplements (e.g., CloneR) could even enhance the cloning performance of StemFit® Basic04 (customer data).

References

StemFit® is recommended by leading Scientists

StemFit® medium was developed 2014 in Japan by the Aminoscience division at Ajinomoto. Since that time, StemFit® has been citied in more than 75 scientific publications so far! The responses from the scientific society, when usingusing the StemFit® media for embryonic stem cells and iPS cells, were very positive . Enclosed a citation from Nakagawa et al., 2014 (Yamanaka’s lab):

„A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cell„

StemFit®, a newly developed Xf-medium, was the best medium for hESC and hiPSC culture with rLN511E8.”

Citations:

Nakagawa, M. et al. (Yamanakas lab) (2014). A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells. Scientific Reports, 4:3594.
Morizane R and Bonventre JV (2017). Generation of nephron progenitor cells and kidney organoids from human pluripotent stem cells. Nature Protocols, 12: 195-207.

Camp JG el al. (2017). Multilineage communication regulates human liver bud development from pluripotency. Nature, 456: 533-538.
Takamichi Miyazaki et al. (2017). Efficient Adhesion Culture of Human Pluripotent Stem Cells Using Laminin Fragments in an Uncoated Manner. Scientific Reports, 7: 41165.

Comparison table

IMPORTANT NOTE: Consolidation of the StemFit® product range
The manufacturer is stopping the international version of the Basic02 and Basic04 media (see announcement here). We recommend the substitution by Basic04 Complete.

Choose your media: Basic04(CT) for basic research and Basic03 or Basic04(CT) for clinical research

StemFit® Basic03
StemFit® Basic04 Complete Type
Single-cell cloning Yes Yes
Free of animal & human material Yes Yes
Basic research Yes
Clinical Research Yes Yes
GMP manufacturing Available In preparation
bFGF Sold separately Included (80 ng/mL)
Number od bottles 2 1

 

Application

Stem cells successfully used with StemFit®:

(based from over 75 scientific reports)

Human embryonic stem cells (hESCs) Human induced pluripotent stem cells (hiPSCs)
253G1 1020C7
KhES1 1027B3
SEES-3 1027B6
SEES-4 1039A1
SEES-5 1120C12
SEES-6 1123C1-G
SEES-7 1156D15
1156D2
more comming soon… 1210B2
1231A3
1335A1
1383D2
1383D6
1383D7
201B7
253G1
253G4
32R1
404C2
409B2
414C2
585A1
585B1
836B1
836B3
987A3
987A7
Ff-I06
Ff-I14
Ff-I01
MIFF3
TIG107
TkDA3-4
more comming soon…

Downloads

Product Information

StemFit Basic04CT_Product Information

Manual

StemFit_Instruction-manual

StemFit with iMatrix-511_Instruction-manual

Safety Data Sheet

StemFit Basic04 Complete Type SDS

FAQ

Which Extracellular Matrix (ECM) is recommended?

Any commercially available ECMs for hPSCs will work (e.g., iMatrix-511, Geltrex, VTN-N, LN521, Matrigel and Synthemax).

How should I switch medium to Basic04?

We recommend to switch to Basic04CT 2 days before passaging.
Please refer to Technical tips for more detail.

What’s the difference between Basic04 and Basic04CT?

Basic04 does not contain bFGF, therefore bFGF can be added and optimized for a specific purpose. Basic04CT does contain bFGF and can be used for PSC expansion without additional supplement.

Can I use Stemfit in a bioreactor?

It is possible to use Basic02 in Bioreactors. There is a presentation deposit available.

How is the performance of StemFit in small clump splitting? Is it possile that the cell walls seem darker and the cell number is reduced?

We recommend single -cell passaging, but small clump passaging is also working. Cell morphologies will always vary among different media. Sometimes the edge of colonies seems “darker” if you use StemFit, but the morphology is always good. Regarding the cell number there are different possibilities:

1.: StemFit tends to make more packed colonies compared to other media, so the actual number would be larger than it’s surface confluency.

2.: In general, adaption to other medium requires several passages. Cell growth would be improved after 2-3 passages.

Which amount of StemFit should I use in a 12-well plate and which cell density is suitable?

In general the medium volume depends on the area of the culture vessels. Therefore, the volume of StemFit would be 750 µL and a cell density of 5,000 – 10,000 cells/well (Depending on the cell line).

However, the evaportaion is sometimes different between different sizes of plates. If cells grow poorly, we would recommend to increase the volume of media up to 1 ml.

3.: Addition of Rock inhibitor on the passaging day will increase cell viability and cell number.

Can I use StemFit for murine cells?

This is not possible, because murine cells require different types of growth factors compared to ES/iPSCs.

What’s the difference between Basic04 and Basic04 Complete Type(Basic04CT)?

Basic04 does not contain bFGF, therefore bFGF can be added and optimized for a specific purpose. Basic04CT does contain bFGF and can be used for PSC expansion without additional supplement.

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