Culture media for stem cells: Recommended by the nobel prize winner Shin’ya Yamanaka:
“StemFit®, a newly developed Xf-medium, was the best medium for hESC and hiPSC culture with rLN511E8.”
Feeder cells and Xeno-free culture medium
StemFit® medium was developed to produce a reliable and well-defined growth condition for stem cells. It contains only Xeno-free components and all necessary components for the culture of embryonic as well as induced Pluripotent Stem (iPS) cells. These important benefits lead to a reduction of variances and contaminations in the cultivation of stem cells, when using the StemFit® medium.
Very reproducible growth rate
The cultivation of stem cells using StemFit® results in a very reproducible growth rate (Fig. 1). This enables a perfect planning of experiments. No more variation due to different starting conditions caused by the natural variation when culturing on feeder cells. Analysing the morphology of stem cells cultivated in StemFit® shows that the colony shape and size are very similar to the cells grown on feeder cells (Fig. 2).
Fig. 1: Reproducible and continuous growth rates of a human iPS-cell line. 201B7 hiPS cells were seeded at different concentrations. The cell number was determined every 7 days for four weeks.
Fig. 2: Colony morphology shows a very similar shape and size of the hiPS-cells when compared to feeder cells.
Less media volume needed
Due to the high-quality components and the ideal concentration of nutrients, the required volume of StemFit® is lower than of conventional media. Additionally, reduced feeding steps during the week, creates a total volume difference between StemFit® and conventional media of more than 50% (Fig 3.).
Fig. 3: The volume of StemFit® can be reduced to by 25% per well. The reduced amount of media changes leads to a further volume reduction of more than 50%.
The maintenance of stem cells is very complicated and labor intense and the feeding step during the weekends is very tedious. StemFit® allows a feeding-free weekend, by using the recommended weekly workflow shown below (Fig. 4).
Fig. 4: StemFit® allows a feeding-free weekend.
Start from a single cell
StemFit® enables a superior colony forming efficiency from a single cell clone, which minimize the effects of stress and result in reliable cells for downstream applications. Furthermore, with StemFit® you can easily determine the efficiency of your cell production and duplicate individual clones.
Easy transition from feeder cells
Majority of the stem cells are still maintained in feeder-cell dependent cultivation. The transition from feeder-cells to nonfeeder-cells system could not be easier: Switch the medium twice before passaging the cells and continue cultivating them in StemFit®. After 2-3 passages the feeder cells will be completely diluted out and a pure stem cell culture will be obtained (Fig. 5).
Fig. 5: Easy transition from feeder-cells to nonfeeder-cells systems with StemFit®.
Compatible with many matrices
StemFit® has been tested on many different matrices. As shown on Fig. 6., the fold expansion rate of cells cultured in StemFit® is much higher when compared to medium A from the market leading competitor.
Fig. 6: Different cell matrices were used as growing matrix and combined with StemFit® or with medium A. The fold expansion of 201B7 hiPS cells was determined after 7 days.
Consistent gene expression profile
The cultivation of stem-cells is very stressful for the cells. Every passage and growth period could therefore introduce unwanted changes in the genome expression profile. Hence, the CGI Catapult Institute in London investigated the genomic profile of StemFit® after 1 passage, 3 and 5 passages and compared it to 4 commercially available media. The most consistent gene expression was obtained using StemFit® (Fig. 7 , StemFit® labeled as AK03N).
Fig. 7: Consistent gene expression when using StemFit® Media (code name AK03N). The expression profile using the TaqMan ScoreCard™ assay (n=3) showed that the most consistent gene expression of after 5 passages were obtained using StemFit® media.
Less production of lactate
The production of lactate is the result of hypoxic stress. The consequences can be changes in the genome expression profile or lead to unwanted differentiation of the stem cells. The CGI Catapult Institute in London showed that there is considerably less lactate production when the cells are grown in StemFit® (Fig. 8).
Fig. 8: StemFit® Media (code name AK03N) caused a considerably lower accumulation of lactate when compared to 4 other commercially available Stem cell media.
High cloning performance
StemFit® Basic02/03 shows a 4-5 higher cloning performance compared to other commercially available media.
The cloning performance of StemFit® Basic04 is about 30% lower than StemFit® Basic02/03, but it is still higher than other commercially available media.
Cloning supplements (e.g., CloneR) could even enhance the cloning performance of StemFit® Basic04 (customer data).