# Bambanker DMSO Free
---
url: https://www.nippongenetics.eu/en/products/cell-biology/freezing-media/dmso-free-media/bambanker-dmso-free/
language: en
type: product
modified: 2026-07-01T08:43:21+02:00
---
## Serum-free cryopreservation media - for most sensitive cells
## Product Details
- **SKU**: BBF01
- **Stock status**: instock
- **Categories**: Cell Biology, Freezing Media, DMSO-free Media
- **Tags**: DMSO free, no stepwise freezing, Serum free
Serum-free freezing media for long storage of cells | DMSO-free – for the most sensitiv cells | Enables long term cell storage at -80°C or -196°C | Improved cell recovery and viability after thawing | Ready-to-use freezing media – no programmed or sequential freezing required | Serum-free – no risk of contamination | Usable for all know cell lines | Long shelf life
- DMSO-free – for the most sensitive cells
- Enables long term cell storage at -80°C or -196°C
- Improved cell recovery and viability after thawing
- Ready-to-use freezing media – no programmed or sequential freezing required
- Serum-free – no risk of contamination
- Manufactured in **GMP-compliant** facilities[ (more information)](#quality)
- Usable for all known cell lines
- Long shelf life
[PRODUCT REQUEST](#section-inquiry_form)
### Details
##### No more DMSO – for the most sensitive cells
DMSO is added to most freezing reagents to help avoid formation of ice crystals, which of course harm the cells. However, DMSO is cytotoxic and can reduce the survival rate of certain sensitive cell lines. Bambanker DMSO Free is made without DMSO. Instead, it uses a unique formulation to avoid the formation of ice crystals. This makes Bambanker DMSO Free especially suitable for cell lines that proved to be sensitive to DMSO under long-term storage conditions.
##### High quality – Manufactured in GMP-compliant facilities
All products of the Bambanker series are manufactured in facilities compliant with current good manufacturing practices (GMP).
The products are subject to a solid quality management system that ensures the consistency, reliability and high quality you can rely on.
Notification: [Manufacturer’s notification for Bambanker as GMP-manufactured product (pdf file)](https://www.nippongenetics.eu/app/uploads/notification-Bambanker-GMP-quality.pdf)
##### Flexible
There a four different versions of Bambanker™.
- All Bambanker™ types are free of fetal bovine serum (FBS) and have a defined and constant composition.
- They also do not contain antibiotic or antifungal ingredients.
The four different types of Bambanker™ are recommended depending on your specific needs:
[Bambanker™](https://www.nippongenetics.eu/en/products/cell-biology/freezing-media/for-all-cell-lines/bambanker-120-ml/) (standard for all cell lines, package units: 20 mL, 5 x 20 mL, 100 mL)
Please note that the 120 mL bottle size (BB01) is no longer available and has been replaced by the new 100 mL bottle size (BB05).[Bambanker™ Direct](https://www.nippongenetics.eu/en/products/cell-biology/freezing-media/for-hybridoma-cells/bambanker-direct-2/) (for hybridoma cells, package unit: 20 mL)[Bambanker™ DMSO Free](https://www.nippongenetics.eu/en/products/cell-biology/freezing-media/dmso-free-media/bambanker-dmso-free/) (for high sensitivity cells, package unit: 20 mL)[Bambanker™ hRM](https://www.nippongenetics.eu/en/products/cell-biology/freezing-media/for-ips-es-cells/bambanker-hrm/) (for ES and iPS cells, package units: 20 mL, 100 mL)
Please note that the 120 mL bottle size (BBH03) is no longer available and has been replaced by the new 100 mL bottle size (BBH04).
### Cell lines & References
##### Cell lines
The following cell lines have successfully been frozen with Bambanker DMSO-free:
1. Jurkat (Floating cells）
2. PC12 (Adrenal Pheochromocytoma, Rat) (Adhesive cells)
3. HepG2 (Adhesive cells)
4. Hela S3 （Epithelioid Carcinoma, Cervix, Human）(Adhesive cells)
5. HEK293T （Transformed Primary Embryonal Kidney, Human）(Adhesive cells)
6. OKT4 (Floating cells）
7. Vero P4 （Kidney, African Green Monkey(Adhesive cells)
8. MDCK (Kidney, Canine）(Adhesive cells)
9. NIH3T3 （Embryonic Fibroblast, Mouse）(Adhesive cells)
##### Customer references
I consider Bambanker a very useful worktool for the following reasons:
1\) replacement of a toxic substance (DMSO)
2\) saving of 4 hours of time required for freezing in nitrogen / -80° C foreseen with the use of DMSO
3\) cellular vitality guaranteed after the defrosting of all the linesthat I manage
_Dott.ssa Allessandra Leone, Department of Virology and Cell Culture, IZSAM “G.Caporale” Teramo_
##### Literature reference
Effect of DMSO on cell differentitation:
Jiang, C. _et al._ Down-regulation of TRRAP-dependent hTERT and TRRAP-independent CAD activation by Myc/Max contributes to the differentiation of HL60 cells after exposure to DMSO _International Immunopharmacology,_ 6 (7), 1204-1213 (2006)
### Downloads
##### Manual
[Manual | Bambanker DMSO-Free (BBF0#)](https://www.nippongenetics.eu/app/uploads/manual-bambanker-dmso-free-bbf0.pdf)
##### Brochure & E-Book
[Brochure | Cryopreservation workflow: Tips and products](https://www.nippongenetics.eu/app/uploads/brochure-cryopreservation-workflow.pdf)
[e-Book | Practical guide to 3D cryopreservation with Bambanker](https://www.nippongenetics.eu/app/uploads/ebook-bambanker-practical-guide-to-3D-cryopreservation.pdf)
##### Flyer
[Flyer Bambanker Freezing Media](https://www.nippongenetics.eu/app/uploads/flyer-bambanker-freezing-media.pdf)
[](https://www.nippongenetics.eu/app/uploads/flyer-bambanker-freezing-media.pdf)
##### Application Note
[Application Note | Bambanker Cell Freezing Media | Overview of cell lines](https://www.nippongenetics.eu/app/uploads/AN-BB-Bambanker-cell-line-table-overview.pdf)
##### MSDS
[MSDS | Bambanker DMSO Free (BBF0#)](https://www.nippongenetics.eu/app/uploads/msds-bambanker-dmso-free-bbf0.pdf)
##### **Certificates of Analysis**
[CoA Finder](https://www.nippongenetics.eu/en/certificates-of-analysis-coas/)
### Rated **5** out of 5 based on 4 customer ratings
4 reviews for Bambanker DMSO Free
1. Rated **5** out of 5
**Eduardo Costa, Centre for Biotechnology and Fine Chemistry, Universidade Católica Portuguesa, Porto** – 02.07.2025
Os meios de congelamento funcionam lindamente e estamos a conseguir recuperar as linhas celulares com viabilidades muito superiores ao tradicional congelamento em FBS.
_Translation by NIPPON Genetis EUROPE:_
The freezing media work beautifully, and we are able to recover the cell lines with much higher viabilities compared to traditional freezing in FBS.
[Verified User](https://www.nippongenetics.eu/en/authenticity-of-reviews/)
2. Rated **5** out of 5
**Desiree Argiriu, Technical University of Munich, Germany** – 12.12.2023
I needed a DMSO-free Medium for my very sinsitive Mastcells and it worked very nice! The medium helped that after freezing and thawing the cells grow faster and have a higher viability. Nice product!
[Verified User](https://www.nippongenetics.eu/en/authenticity-of-reviews/)
3. Rated **5** out of 5
**Dr. Céline Laschet, BCiPharma, Belgium** – 25.07.2023
We observed that there were more living cells after thawing (4.5 times more cells after 3 days of thawing) but not yet enough cells to pass into a T75. It took 7 days of thawing for the passage in a T75 vs 10 days for our classic freezing condition.
[Verified User](https://www.nippongenetics.eu/en/authenticity-of-reviews/)
4. Rated **5** out of 5
**Mady, Tübingen University, Germany** – 11.07.2017
We had a major liquid Nitrogen tank issue. We looked for an alternative. We requested a free sample to try.
Bambanker was sooo easy to use and very good on sensitive cells (no toxicity see), which can also be stored at -80. No more DMSO/FBS!!
I highly recommend it (10 years+ (primary& cell line) cell culture experience!)
[Verified User](https://www.nippongenetics.eu/en/authenticity-of-reviews/)
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