# MIDORI Green Xtra --- url: https://www.nippongenetics.eu/en/products/electrophoresis-dna-rna/nucleic-acid-stains/midori-green-xtra/ language: en type: product modified: 2026-06-17T02:55:21+02:00 --- ## Safe DNA/RNA stain ## Product Details - **SKU**: MG10 - **Stock status**: instock - **Categories**: DNA/RNA Electrophoresis, Nucleic Acid Stains ## Attributes - **Type of staining**: In-gel and post-staining - **Light source**: Blue/Green LED Ultra-sensitive DNA stain for the detection of DNA/RNA in agarose gels | Flexible: For in-gel staining and post staining | Safe alternative to ethidium bromide: Non-carcinogenic and non-toxic | Optimal for Blue/Green LED technology and Blue LED light. - Ultra-sensitive DNA stain for the detection of DNA/RNA in agarose gels - Flexible: For in-gel staining and post staining - Safe alternative to ethidium bromide: Non-carcinogenic and non-toxic - Optimal for Blue/Green LED technology and Blue LED light. (For UV light we recommended [MIDORIGreen Advance](https://www.nippongenetics.eu/en/product/midori-green-advance/).) ### Details ##### MIDORIGreen Xtra – Non-carcinogenic DNA stain of the latest generation – Ultra-sensitive for LED illumination MIDORIGreen Xtra is a new highly sensitive green fluorescent stain for a safe visualization of DNA and RNA in agarose gels. This DNA stain is a safe and better alternative to the traditional nucleic acid stain ethidium bromide (EtBr). Remarkably, agarose gels stained with MIDORIGreen Xtra have a very low background fluorescence, which makes the identification of low amounts of DNA very easy. Be one of the first and test MIDORIGreen Xtra for a safe detection of DNA with an unbeatable sensitivity. ![Ultra-sensitive DNA signals with the DNA stain MIDORI Green Xtra](https://www.nippongenetics.eu/app/uploads/2018/08/MG10_MIDORI-Green-Xtra_Gele-mit-abgerundeten-Kanten-300x129.png)Ultra-high sensitivity of DNA bands detected with MIDORI Green Xtra. Left: Detection with a Blue/Green LED transilluminator. Right: Detection with a Blue LED transilluminator. ##### Ultra-sensitive DNA signals and low background – Just a perfect signal MIDORIGreen Xtra is optimized for Blue/Green and Blue LED light, leading to unbeatable fluorescence signals of DNA and RNA in agarose gels. In addition, UV-light is also suitable for the detection of nucleic acid, but less efficient than non-damaging visible light. Remarkably, MIDORIGreen Xtra did not stain the agarose gel, leading to an excellent signal to noise ratio. ##### Safe Alternative to Ethidium Bromide MIDORIGreen Xtra delivers even better DNA/RNA signals than ethidium bromide. However, this innovative DNA stain is non-carcinogenic, non-mutagenic and non-toxic and therefore not harmful for your health. Independent laboratories confirmed its safety: Both the mutagenicity test (ames test) and the cytotoxicity test were negative. Convince yourself and download here our [safety report](https://www.nippongenetics.eu/app/uploads/2018/08/Midori-Green-Xtra-Safety-Report.pdf). ### Compatibility MIDORIGreen Xtra is optimized for Blue/Green and Blue LED light, leading to unbeatable fluorescence signals of DNA and RNA in agarose gels. MIDORIGreen Xtra is suitable for In-Gel and Poststaining. MIDORI Green DNA DyeBlue/Green LEDBlue LEDUV-LightIn-GelPoststainingDirectAdvance (MG04)![Compatible](https://www.nippongenetics.eu/app/uploads/2017/01/Midori-gruen-e1613117293365.png)![Compatible](https://www.nippongenetics.eu/app/uploads/2017/01/Midori-gruen-e1613117293365.png)![Compatible](https://www.nippongenetics.eu/app/uploads/2017/01/Midori-gruen-e1613117293365.png)![Compatible](https://www.nippongenetics.eu/app/uploads/2017/01/Midori-gruen-e1613117293365.png)![Compatible](https://www.nippongenetics.eu/app/uploads/2017/01/Midori-gruen-e1613117293365.png)![Not Compatible](https://www.nippongenetics.eu/app/uploads/2017/01/Midori-rot-e1613117305907.png)Direct (MG06)![Compatible](https://www.nippongenetics.eu/app/uploads/2017/01/Midori-gruen-e1613117293365.png)![Compatible](https://www.nippongenetics.eu/app/uploads/2017/01/Midori-gruen-e1613117293365.png)![Not Recommended](https://www.nippongenetics.eu/app/uploads/2017/01/Midori-gelb-e1613117324663.png)![Not Compatible](https://www.nippongenetics.eu/app/uploads/2017/01/Midori-rot-e1613117305907.png)![Not Compatible](https://www.nippongenetics.eu/app/uploads/2017/01/Midori-rot-e1613117305907.png)![Compatible](https://www.nippongenetics.eu/app/uploads/2017/01/Midori-gruen-e1613117293365.png)Xtra (MG10)![Compatible](https://www.nippongenetics.eu/app/uploads/2017/01/Midori-gruen-e1613117293365.png)![Compatible](https://www.nippongenetics.eu/app/uploads/2017/01/Midori-gruen-e1613117293365.png)![Not Recommended](https://www.nippongenetics.eu/app/uploads/2017/01/Midori-gelb-e1613117324663.png)![Compatible](https://www.nippongenetics.eu/app/uploads/2017/01/Midori-gruen-e1613117293365.png)![Compatible](https://www.nippongenetics.eu/app/uploads/2017/01/Midori-gruen-e1613117293365.png)![Not Compatible](https://www.nippongenetics.eu/app/uploads/2017/01/Midori-rot-e1613117305907.png)![Compatible](https://www.nippongenetics.eu/app/uploads/2017/01/Midori-gruen-e1613117293365.png) Compatible ![Not Recommended](https://www.nippongenetics.eu/app/uploads/2017/01/Midori-gelb-e1613117324663.png) Compatible, but not recommended ![Not Compatible](https://www.nippongenetics.eu/app/uploads/2017/01/Midori-rot-e1613117305907.png) Not compatible ### Free sample ### Downloads ##### Manual [Manual | MIDORI Green Xtra (MG10, MG09)](https://www.nippongenetics.eu/app/uploads/MG10-MG09-Midori-Green-Xtra-Manual.pdf) ##### Product Flyer [Flyer – MIDORI Green Safe DNA Stain](https://www.nippongenetics.eu/app/uploads/flyer-midori-green-safe-dna-stain.pdf) ##### MSDS [Safety Data Sheet](https://www.nippongenetics.eu/app/uploads/MSDS-Midori-Green-Xtra-MG10.pdf) ##### Safety Report [Safety Report – MIDORI Green Xtra](https://www.nippongenetics.eu/app/uploads/safety-report-midori-green-xtra.pdf) ##### Technical Notes [Technical Note | Comparison of MIDORI Green dyes and ethidium bromide](https://www.nippongenetics.eu/app/uploads/tcn-mg04-mg10-comparison-of-midori-green-with-etbr.pdf) [Pre-staining of DNA with MIDORIGreen Xtra (Technical Note 2018\_06)](https://www.nippongenetics.eu/app/uploads/2019/12/MG10_Midori-Green-Xtra_Technical-Note-2018_06.pdf) [Post-staining of DNA with MIDORIGreen Xtra (Technical Note 2019\_01)](https://www.nippongenetics.eu/app/uploads/2019/12/MG10_Midori-Green-Xtra_TN_2019_01.pdf) [Storage of MGX stained Gels (TN 2019\_04)](https://www.nippongenetics.eu/app/uploads/2020/07/MG10_Midori-Green-Xtra_TN_2019_4.pdf) ##### Certificates of Analysis [CoA Finder](https://www.nippongenetics.eu/en/certificates-of-analysis-coas/) ### FAQ ##### I have seen a white smear in my agarose gels using Midori Green Xtra and Orange G as loading dye. We know that the combination of Midori Green Xtra and Orange G will lead to a white smear while using UV light. To avoid this, please use Blue or Blue/Green LED detection systems or use a different loading dye. ##### I want to separate fragments of around 100 bp. So I set up the run at 100V for 1.5h. Now I can see that the stain is migrating out of the gel. What do you recommend? The dye is oppositely charged to the DNA, so if the DNA migrated downwards, the stain will migrate upwards. Therefore, the gel will destain. We recommend to use a higher agarose percentage and a quicker run. ##### The stain is precipitating after storage at 4°C. What do you recommend. We commend to warm the stain up to RT or 37°C for several minutes. Please vortex the stain before usage. ### Rated **5** out of 5 based on 9 customer ratings 9 reviews for MIDORI Green Xtra 1. ![](https://secure.gravatar.com/avatar/aaf2dbd51994356afbb21008cba68cf511ec488bb489292a528c6b761ba36261?s=60&d=mm&r=g)Rated **5** out of 5 **Mint-Campus Alte Schmelz** – 06.08.2025 Wir machen eine PCR zum Nachweis von Bakterien (Lactobacillen aus Joghurt) und zur Geschlechtsbestimmung aus Vogelfedern als Schulversuch bzw. Jugend Forscht-Arbeit. Das bedeutet für uns, der Farbstoff muss sicher sein: ungiftig und nicht mutagen, er muss passen auf unseren Transilluminator blaue LEDs (ohne UV) und sollte eine hohe Sensitivität haben. Die Schüler haben zum ersten Mal eine Pipette in der Hand, dann sollte man auch was sehen, wenn der Pipettierfehler bis zu 20 % ist 😉 und das klappt. Daher möchten wir dem Produkt 5\* geben und sind zufrieden damit. Translation by NIPPON Genetics EUROPE We are conducting a PCR test to detect bacteria (lactobacilli from yoghurt) and to determine the sex of birds from their feathers as part of a school experiment and a Jugend Forscht project. This means that the dye must be safe: non-toxic and non-mutagenic, it must be compatible with our transilluminator blue LEDs (without UV) and should have a high sensitivity. The students are using a pipette for the first time, so you should be able to see something even if the pipetting error is up to 20% 😉 and it works. That’s why we would like to give the product 5\* and are satisfied with it. [Verified User](https://www.nippongenetics.eu/en/authenticity-of-reviews/) 2. ![](https://secure.gravatar.com/avatar/045ce081e26bebc864f36cdc8b90d3771382900ae4d1710e61b0d5dfab3a693d?s=60&d=mm&r=g)Rated **5** out of 5 **Cynthia Geelen, KNAW, Netherlands Institute for Neuroscience** – 20.06.2024 I am amazed at how well Midori Green performs compared to Ethidium Bromide, which I have been using throughout my working career. The comparable results have made it an easy choice for me to switch to create a safer workplace. [Verified User](https://www.nippongenetics.eu/en/authenticity-of-reviews/) 3. ![](https://secure.gravatar.com/avatar/045ce081e26bebc864f36cdc8b90d3771382900ae4d1710e61b0d5dfab3a693d?s=60&d=mm&r=g)Rated **5** out of 5 **Diagnostik Labor, Hamburg** – 06.10.2021 Sehr schön zu sehen ist, dass der Xtra-Farbstoff deutlich besser in der Sensitivität und im fast nicht gefärbten Hintergrund ist. Neben den Markers ist BSA aufgetragen, um zu sehen, ob wir hier auch Proteine aus z.B. Restriktionsverdauen färben. Aber auch das ist sehr positiv, da die Proteine nicht gefärbt werden. Wir haben uns auch schon den Midori Green Xtra-Farbstoff bei Ihnen bestellt und wechseln jetzt dauerhaft auf diesen. Translation by NIPPON Genetics EUROPE: It is very nice to see that the Xtra dye is significantly better in sensitivity and in the almost non-colored background. In addition to the markers, BSA is applied to see whether we are also staining proteins from e.g. restriction digests. But this is also very positive because the proteins are not colored. We have already ordered the Midori Green Xtra dye from you and are now permanently switching to it. [Verified User](https://www.nippongenetics.eu/en/authenticity-of-reviews/) 4. ![](https://secure.gravatar.com/avatar/?s=60&d=mm&r=g)Rated **5** out of 5 **Researcher at University of Münster (WWU), Germany** – 08.09.2020 A very safe and sensitive dye. Works extremely good with the blue/green LED. Havent tested it on a blue table, but sensitivity is much better then other ethidiumbromide alternatives we tested. It even works extremely well with light on in the lab using the amber shield or googles to extract the bands from the gel. We are completely satisfied and convinced. [Verified User](https://www.nippongenetics.eu/en/authenticity-of-reviews/) 5. ![](https://secure.gravatar.com/avatar/be2a6c9a656e7fc4d68b81e18a4e5d6bd3dd28f0802b6fc7110b839ba88bc616?s=60&d=mm&r=g)Rated **5** out of 5 **Matt from Nextbiotics** – 06.08.2019 Midori Green Xtra shows fantastic performance in the lab and is half the price per gel of SYBR Safe, so we’ll be switching to this awesome dye for all of our agarose gels moving forward! [Verified User](https://www.nippongenetics.eu/en/authenticity-of-reviews/) 6. ![](https://secure.gravatar.com/avatar/?s=60&d=mm&r=g)Rated **5** out of 5 **IPK Gatersleben, Plant Reproductive Biology** – 15.05.2019 This stain is the first real replacement for ethidiumbromide. So I´m satisfied. [Verified User](https://www.nippongenetics.eu/en/authenticity-of-reviews/) 7. ![](https://secure.gravatar.com/avatar/be2a6c9a656e7fc4d68b81e18a4e5d6bd3dd28f0802b6fc7110b839ba88bc616?s=60&d=mm&r=g)Rated **5** out of 5 **Teacher from private high school in Japan** – 10.04.2019 In class experiments, it is important to have all three results of “Successful experiment”, “Easy to get the result” and “Easy handling (safe)” at the same time. In that respect, MIDORI Green Xtra is significantly superior to other products. Perfectly fit to FAS-Nano’s Blue/Green LED, and the band was clearly visible. With this instrument, you can take an image with iPad and see the results immediately while giving an explanation of electrophoresis result to my students. Blue LED also worked with this dye by the way. [Verified User](https://www.nippongenetics.eu/en/authenticity-of-reviews/) 8. ![](https://secure.gravatar.com/avatar/be2a6c9a656e7fc4d68b81e18a4e5d6bd3dd28f0802b6fc7110b839ba88bc616?s=60&d=mm&r=g)Rated **5** out of 5 **Japanese Researcher from National Cancer Research Center** – 28.03.2019 Compared to the Etidium Bromide, the sensitivity is absolutely fine with having better safety, and also easier handling than the Midori Green Direct. I really like this dye because it can be used with both In-gel staining and post-staining. [Verified User](https://www.nippongenetics.eu/en/authenticity-of-reviews/) 9. ![](https://secure.gravatar.com/avatar/be2a6c9a656e7fc4d68b81e18a4e5d6bd3dd28f0802b6fc7110b839ba88bc616?s=60&d=mm&r=g)Rated **5** out of 5 **Japanese Researcher, Jichi Medical University Department of Regenerative Medicine, Shimotsuke, Japan** – 01.02.2019 Overwhelming results with Blue LED light. Much better than Ethidium bromide! [Verified User](https://www.nippongenetics.eu/en/authenticity-of-reviews/) Only logged in customers may leave a review.