# FastGene Plasmid Mini Kit --- url: https://www.nippongenetics.eu/en/products/nucleic-acid-purification/plasmid-dna/fastgene-plasmid-mini-kit/ language: en type: product modified: 2026-01-05T10:02:12+01:00 --- ## Plasmid Mini prep kit ## Product Details - **SKU**: FG-90402, FG-90502 - **Stock status**: instock - **Categories**: Nucleic Acid Purification, Plasmid DNA - **Tags**: Fast protocol, High and low copy plasmids, LB tablets included ## Attributes - **Package unit**: 100 preps, 300 preps Rapid small scale isolation and purification of high copy and low copy plasmid DNA | High yields of plasmid DNA | Cost effective preparations | LB-Broth capsules included - Rapid small scale isolation and purification of high copy and low copy plasmid DNA - High yields of plasmid DNA - Cost effective preparations - LB-Broth capsules included ### Details **FastGene® Plasmid Mini Kits** are designed for rapid small scale isolation and purification of high copy and low copy plasmid DNA. The ready-to-use plasmid DNA is of high quality in low-salt Tris buffer and suitable for typical downstream applications: Cloning, sequencing, PCR, transformation and restriction analysis. ##### One kit with all components Each kit comes with ready-to-use LB-Broth capsules. Add one LB-Broth capsule in 40 mL water, autoclave your solution and start your cloning experiment. The kit includes everything that is needed for a plasmid preparation. ![](https://www.nippongenetics.eu/app/uploads/2017/03/LB01_LB-Broth-Capsules-100-150x150.jpg) ##### High and low-copy plasmid DNA preparation The FastGene® Plasmid Mini Kit is designed for rapid small scale isolation and purification of high copy and low copy plasmid DNA. The purified plasmid DNA is of high quality and ready to use in low-salt Tris buffer, suitable for typical downstream applications: Cloning, sequencing, PCR, transformation and restriction analysis. ##### High yield – High purity – Fast preparation The FastGene® Plasmid Mini Kits are faster than competitors with comparable yield. This allows you to save time and perform your downstream application quicker. The gel demonstrates that FastGene® Plasmid Mini Kits yield equal amount of plasmid DNA compared to other supplier with even more time consuming protocols. The preparation with the FastGene® Plasmid Mini Kit was performed by using the Fast Protocol. ![FastGene® Plasmid Mini Kit - Ausbeute-Vergleich ](https://www.nippongenetics.eu/app/uploads/2016/05/plasmid-gelbild-mit-beschriftung0_small.jpg) Fig. 1: pBluescript plasmid DNA was isolated from a 1,4 ml E.coli culture (DH5α in LB medium) according to the recommended procedures of different kits and eluted in 50 µl elution buffer. 2 µl of each eluate were loaded on a 0,7% TAE agarose gel. ![Sequencing result pf plasmid dna purified with FastGene Plasmid Mini Kit](https://www.nippongenetics.eu/app/uploads/2016/05/plasmid_sequencedata00_1100x740-300x202.jpg) Fig.2: Typical fluorescent capillary sequencing result from pBluescript plasmid DNA purified using the FastGene® Plasmid Mini Kit. Cycle sequencing reaction was performed on an ABI3100 sequenzer. 1 µl of purified plasmid DNA was used as a template. Dye terminators were removed by [FastGene® Dye Terminator Removal Kit](https://www.nippongenetics.eu/en/produkte/nucleic-acid-purification/dye-terminator-removal/fastgene-dye-terminator-removal-kit-50/) ### Specifications **Parameter****High-copy plasmid****Low-copy plasmid****Max. sample volume**1-5 mL over-night-culture5-10 mL over-night-culture**Typical yield**< 25 µg< 25 µg**Elution volume**50 µl50 µl**Binding capacity**40 µg40 µg**Size of vector**< 15 kb< 15 kb**Prep time**26 min / 12 preps26 min / 12 preps**Format**Spin columnSpin column### Protocols ![](https://www.nippongenetics.eu/bilder/products/manuelle-dna-extraktion/plasmid-mini-kit/fgplasmidqguide0_810x10000_810x1000.jpg) ### Documents ##### Manuals [QuickGuide FastGene Plasmid Mini Kit (FG-90)](https://www.nippongenetics.eu/app/uploads/2017/01/FGPlasmidminikit-QuickGuide.pdf) [Manual FastGene Plasmid Mini Kit (FG-90)](https://www.nippongenetics.eu/app/uploads/2017/01/FG-90_FG-Plasmid-Mini-Kit_Manual.pdf) ##### MSDS [MSDS | FastGene Plasmid Mini Kit | FG-90](https://www.nippongenetics.eu/app/uploads/msds-fastgene-plasmid-mini-kit-fg-90.pdf) ##### Application Notes [Application Note – Customers product feedback](https://www.nippongenetics.eu/app/uploads/2019/11/FG-90_FG-Plasmid-Mini-Kit_AN_2016_27_en.pdf) [Application Note – Purification of plasmids using FastGene® Plasmid Mini Kit](https://www.nippongenetics.eu/app/uploads/2017/03/FG-90502_FG-Plasmid-Mini-Kit_Purification-of-plasmids-using-FastGene®-Plasmid-Mini-Kit.pdf) ### FAQ ##### Can the Plasmid Mini Kit be used with the Qiacube automated system? We would not recommend to use the Plasmid Kit with the Qiacube. The problem is the fitting of the tube. It must fit perfectly, otherwise it could lead to leaking of the tubes. ### Reviews There are no reviews yet. Only logged in customers may leave a review.