# DNAreleasy Advance --- url: https://www.nippongenetics.eu/en/products/pcr-reagents-and-enzymes/endpoint-pcr/direct-pcr/dnareleasy-advance-1-5-ml-50-rxns/ language: en type: product modified: 2026-04-29T09:19:31+02:00 --- ## Product Details - **SKU**: LS06 - **Stock status**: instock - **Categories**: PCR Reagents and Enzymes, Endpoint PCR, Direct PCR - **Tags**: automatable, Direct PCR, time saving - 1.5 mL, 50 reactions - PCR done the easy way - Successful lysis of different biological material - Very easy to use ### Details ##### From cells to PCR in less than 15 minutes DNAreleasy Advance replaces time-consuming and difficult extraction methods. The released DNA can directly be used for a PCR reaction or stored for several months at -20 °C. Are you tired of the time-consuming extraction processes and costly spin columns that you’ve been using to prepare samples for DNA amplification? With the DNAreleasy Advance Direct Lysis Kit, we now offer a better solution. The new cell lysing reagent only requires a 15 minutes incubation in a thermal cycler before the DNA is ready-to-use directly for your PCR — without any further sample processing! ##### Successfully used samples - Saliva - Hair roots - Animal tissue (horse, pig liver, etc.) - Mouse tails and ears - Plants (leaf, blossom, pollem): Cabbage, maize, canola, soy, sugar beet, etc. - Drosophila - Yeast - Mollusca ### Applications ##### Genomic DNA from scallops Genomic DNA scallops was isolated by DNAreleasy Advance and a part of the supernatant was directly added to the PCR reaction. The agarose gel shows the high yield of amplified DNA fragment. ![](https://www.nippongenetics.eu/app/uploads/2016/05/dnareleasy-advance_gel_eurofins0_287x1690_287x169.jpg) The same amplified DNA was used for subsequent sequencing experiments. ![](https://www.nippongenetics.eu/app/uploads/2016/05/dnareleasy-advance_seq_eurofins0_1000x5910_1000x591.jpg) Data kindly provided by Dr. Schubbert, Eurofins Medigenomix GmbH, Ebersberg, Germany ##### Quantitative PCR of plant tissues Total DNA was isolated by DNAreleasy Advance from various plant species. Thereafter the melting curve was determined by RT PCR by using a Light Cycler instrument (Roche): ![](https://www.nippongenetics.eu/app/uploads/2016/05/dnareleasy-advance-plant-melt-peak-web0_500x2740_500x274.jpg) Positive control (PTC), maize meal, wheat flour, sugar beet, white cabbage; negative control (NTC) ##### Human and animal genomic DNA Genomic DNA was isolatedby using DNAreleasy Advance from different materials and organism and analyzed by Roche Light Cycler: ![](https://www.nippongenetics.eu/app/uploads/2016/05/dnareleasy-advance-quant-cytb-web0_747x4160_747x416.jpg) (1) positive control human DNA, (2) saliva, (3) hair root, (4) pig liver, (5) drosophila melangogaster, (6) horse meat ### Downloads ##### Manual [Manual | DNAreleasy Advance | LS06](https://www.nippongenetics.eu/app/uploads/manual-dnareleasy-advance-ls05-ls06.pdf) ##### MSDS [MSDS | DNAreleasy Advance | LS06](https://www.nippongenetics.eu/app/uploads/msds-dnareleasy-advance-ls06.pdf) ### FAQ ##### Does the lysing solution contain proteinase K? Yes, it contains proteinase K. ##### After the lysis and running my PCR, I have seen a huge band of genomic DNA. How I can get rid of it? The best idea would be to dilute the template up to 1:1000. It it possible that the amount of DNA is to high ,which will lead to a template inhibition. ### Rated **5** out of 5 based on 1 customer rating 1 review for DNAreleasy Advance 1. ![](https://secure.gravatar.com/avatar/045ce081e26bebc864f36cdc8b90d3771382900ae4d1710e61b0d5dfab3a693d?s=60&d=mm&r=g)Rated **5** out of 5 **Viral Vector Core Facility, University of Oxford** – 13.08.2024 We have been using DNA Releasy Advance since August 2023. We use it to recover a suitable DNA sample from purified virus, to use for PCR as part of our QC process. We find it to be a very reliable product which produces consistent results. We value product dependability, and a straightforward ordering and fast despatch process. [Verified User](https://www.nippongenetics.eu/en/authenticity-of-reviews/) Only logged in customers may leave a review.