# FastGene HiFi HS 2x Master Mix --- url: https://www.nippongenetics.eu/en/products/pcr-reagents-and-enzymes/endpoint-pcr/hifi-polymerase/fastgene-hifi-hs-2x-master-mix/ language: en type: product modified: 2026-01-05T10:02:55+01:00 --- ## High fidelity HS (Hot Start) DNA polymerase ## Product Details - **SKU**: LS36 - **Stock status**: instock - **Categories**: PCR Reagents and Enzymes, Endpoint PCR, HIFI Polymerase - **Tags**: 500 Units, PCR Enzymes, Taq Polymerase ## Attributes - **Template**: DNA - **Application**: High GC-content, Genotyping, HotStart, High Fidelity - **Technique**: End-point PCR Reliable high fidelity polymerase | Superior performance under difficult conditions, e.g., AT or GC-rich sequences and long templates | Perfect for mulitplex-PCRs and applications where high accuracy is needed (e.g., cloning, site-directed mutagenesis, sequencing) | Ready-to-use cocktail including all components neccessary for a wide range of targets and fragment sizes | 3’-5’ exonuclease and proof-reading activity - Reliable high fidelity polymerase - 100x higher fidelity than Taq DNA polymerase - Special hot start technology for maximum sensitivity and specificity (see below) - Superior performance under difficult conditions, e.g., AT or GC-rich sequences and long templates (up to 17.5 kb) - Perfect for mulitplex-PCRs and applications where high accuracy is required (e.g., cloning, site-directed mutagenesis, sequencing) - Ready-to-use cocktail with all components neccessary for a wide range of targets and fragment sizes - 3’-5’ exonuclease and proof-reading activity - Easy experimental set-up at room temperature - Stability of the reaction mixture for up to 24 hours before and after the PCR run ### Details ##### High fidelity is the key The high fidelity of the FastGene® HiFi HS (Hot Start) Polymerase is based on the improved 3`-5` exonuclease activity, which significantly reduces the error rate of the enzyme and makes it around 100 times more accurate than a Taq DNA polymerase. This is ideal for applications where high fidelity is essential, such as sequencing, cloning and site-directed mutagenesis. ##### Superior hot start technology The FastGene® HiFi HS Polymerase uses a special technology with an aptamer-like molecule that gives the polymerase hot start activity that is different from the antibody-mediated hot start normally used. The aptamer-like molecule reversibly binds and inhibits the 3′-5′ exonuclease and 5′-3′ polymerase activities of the enzyme at temperatures below 40°C. This sophisticated hot start molecule prevents primer dimer formation and non-specific amplification. It therefore maximises the sensitivity and specificity of your PCR and allows easy experimental set-up at room temperature. ##### Save time with the Master Mix The FastGene® HiFi Polymerase is provided in a 2x Master Mix, which significantly speeds up the preparation of a PCR. It contains an advanced buffering system with dNTPs, Mg2+, reaction enhancers and the polymerase enzyme. ##### Product applications The FastGene® HiFi HS 2x Master Mix (LS36) is ideally suited for: - Accurate Cloning - Sequencing - Site-directed mutagenesis - High fidelity PCR - Long range PCR - Any standard PCR application for which a hot start formulation of a high-quality thermostable DNA polymerase is require ##### Challenging PCR? Perfect for FastGene® HiFi Polymerase! The FastGene® HiFi Polymerase is the high-fidelity enzyme for precise PCR amplifications. The enzyme was engineered in a way that it can amplify particularly **long templates (up to 17.5 kb)** with a high sequence accuracy. Furthermore, it shows a significant improvement in extension times (10-30 sec per kb), while generating high yields, even with difficult templates. ##### Amplify long fragments ![](https://www.nippongenetics.eu/app/uploads/ls36-fastgene-hifi-polymerase-long-fragments-300x204.png) _Figure 1: The FastGene® HiFi Polymerase is capable of amplifying a long range of fragments, even up to 17500 bp. (The image was generated with [MIDORIGreen Xtra](https://www.nippongenetics.eu/en/products/electrophoresis-dna-rna/nucleic-acid-stains/midori-green-xtra/) safe DNA dye and [FastGene® FAS-DIGI PRO](https://www.nippongenetics.eu/en/products/gel-documentation/gel-documentation-systems/high-end/fastgene-fas-digi-pro/) gel documentation system.)_ ##### Deal with tough templates ![](https://www.nippongenetics.eu/app/uploads/ls36-fastgene-hifi-polymerase-gc-content-300x193.png) _Figure 2: Templates with varying GC-content, ranging from ~30 % – 80 % can be successfully amplified with the FastGene® HiFi Polymerase. (The image was generated with [MIDORIGreen Xtra](https://www.nippongenetics.eu/en/products/electrophoresis-dna-rna/nucleic-acid-stains/midori-green-xtra/) safe DNA dye and [FastGene® FAS-DIGI PRO](https://www.nippongenetics.eu/en/products/gel-documentation/gel-documentation-systems/high-end/fastgene-fas-digi-pro/) gel documentation system.)_ ##### Perform easy multiplexing ![](https://www.nippongenetics.eu/app/uploads/ls36-fastgene-hifi-ppolymerase-multiplexing-242x300.png) _Figure 3: 6 phage DNA fragments, 4 mouse DNA fragments and a combination of both were successfully amplified with the FastGene® HiFi Polymerase in a multiplex PCR. (The image was generated with [MIDORIGreen Xtra](https://www.nippongenetics.eu/en/products/electrophoresis-dna-rna/nucleic-acid-stains/midori-green-xtra/) safe DNA dye and [FastGene® FAS-DIGI PRO](https://www.nippongenetics.eu/en/products/gel-documentation/gel-documentation-systems/high-end/fastgene-fas-digi-pro/) gel documentation system.)_ ### Free sample ##### Convince yourself and ask for a free sample! ##### ### Downloads ##### Manual [Manual – FastGene® HiFi HS 2x Master Mix (LS36)](https://www.nippongenetics.eu/app/uploads/manual-ls36-fg-hifi-hs-2x-master-mix.pdf) ##### MSDS [MSDS | FastGene HiFi HS 2x Master Mix | LS36](https://www.nippongenetics.eu/app/uploads/msds-fg-hifi-hs-2x-master-mix-ls36.pdf) ### Reviews There are no reviews yet. Only logged in customers may leave a review.