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Nippon Genetics is proud to introduce a novel reagent specially formulated in order to eliminate nucleic acid purification for PCR analysis. DNAreleasy Advance is a continuous development of our well know lysis reagent DNAreleasy suitable for template preparation of PCR experiments. DNAreleasy Advance replaces time consuming and tedious extractionand purification methods. The released DNA can be used directly in a PCR reaction or can be stored at -20 °C for several months. So far DNAreleasy has been tested on various cell types.
Genomic DNA from scallops
Genomic DNA scallops was isolated by DNAreleasy Advance and a part of the supernatant was directly added to the PCR reaction. The agarose gel shows the high yield of amplified DNA fragment.
The same amplified DNA was used for subsequent sequencing experiments.
Data kindly provided by Dr. Schubbert, Eurofins Medigenomix GmbH, Ebersberg, Germany
Quantitative PCR of plant tissues
Total DNA was isolated by DNAreleasy Advance from various plant species. Thereafter the melting curve was determined by RT PCR by using a Light Cycler instrument (Roche):
Positive control (PTC), maize meal, wheat flour, sugar beet, white cabbage; negative control (NTC)
Human and animal genomic DNA
Genomic DNA was isolatedby using DNAreleasy Advance from different materials and organism and analyzed by Roche Light Cycler:
(1) positive control human DNA, (2) saliva, (3) hair root, (4) pig liver, (5) drosophila melangogaster, (6) horse meat
DNAreleasy Advance replaces time-consuming and difficult extraction methods. The released DNA can directly be used for a PCR reaction or stored for several month at – 20 °C.
Does the lysing solution contain proteinase K?
Yes, it contains proteinase K.
After the lysis and running my PCR, I have seen a huge band of genomic DNA. How I can get rid of it?
The best idea would be to dilute the template up to 1:1000. It it possible that the amount of DNA is to high ,which will lead to a template inhibition.
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