# Clara Probe Mixes --- url: https://www.nippongenetics.eu/en/products/pcr-reagents-and-enzymes/qpcr/fastgene-probe-qpcr/clara-probe-mixes/ language: en type: product modified: 2026-09-15T13:30:27+02:00 --- ## Highly sensitive 4x master mix for reliable singleplex and multiplex probe-based qPCR ## Product Details - **On sale**: yes - **Stock status**: instock - **Categories**: PCR Reagents and Enzymes, Quantitative PCR, Probe qPCR ## Attributes - **Passive reference**: Lo-ROX, Hi-ROX, No-ROX, Separate-ROX - **Package unit**: 1 x 1 mL (200 x 20 µL rxns), 3 x 1 mL (600 x 200 µL rxns), 5 x 1 mL (1000 x 20 µL rxns), 1 x 50 mL (10000 x 20 µL rxns) - **Concentrated 4x master mix** for flexible assay setup - Reliable detection of **low template amounts** - Designed for **singleplex and multiplex qPCR** - Antibody-mediated **hot start Taq DNA polymerase** - Compatible with **standard and fast cycling** - Available with multiple **ROX formats** ### Details ##### Reliable probe qPCR – from routine assays to multiplex workflows Clara® Probe Mix is a concentrated 4x master mix for sensitive probe-based DNA detection. The optimised formulation supports reproducible amplification across both singleplex and multiplex assays while leaving additional reaction volume available for template, primers and probes. Powered by an antibody-mediated hot start Taq DNA polymerase, Clara® Probe Mix is compatible with a broad range of probe chemistries and standard or fast-cycling real-time PCR instruments. It is suitable for routine research workflows as well as more complex high-throughput and multiplex assay development. ##### Applications - Singleplex and multiplex DNA detection - 2-step RT-qPCR - Gene expression analysis - Genotyping - Allelic discrimination - Species abundance quantification - qPCR assay development - In vitro diagnostic assay development ##### Ordering information **Product****ROX****Package unit****Cat. no.**Clara® Probe MixLo-ROX1 x 1 mL | 200 x 20 µL rxnsPB20.61-01Lo-ROX3 x 1 mL | 200 x 20 µL rxnsPB20.61-03Lo-ROX5 x 1 mL | 200 x 20 µL rxnsPB20.61-05Lo-ROX1 x 50 mL | 200 x 20 µL rxnsPB20.61-50Clara® Probe MixHi-ROX1 x 1 mL | 200 x 20 µL rxnsPB20.62-01Hi-ROX3 x 1 mL | 200 x 20 µL rxnsPB20.62-03Hi-ROX5 x 1 mL | 200 x 20 µL rxnsPB20.62-05Hi-ROX1 x 50 mL | 200 x 20 µL rxnsPB20.62-50Clara® Probe MixNo-ROX1 x 1 mL | 200 x 20 µL rxnsPB20.63-01No-ROX3 x 1 mL | 200 x 20 µL rxnsPB20.63-03No-ROX5 x 1 mL | 200 x 20 µL rxnsPB20.63-05No-ROX1 x 50 mL | 200 x 20 µL rxnsPB20.63-50Clara® Probe MixSeparate-ROX1 x 1 mL | 200 x 20 µL rxnsPB20.64-01Separate-ROX3 x 1 mL | 200 x 20 µL rxnsPB20.64-03Separate-ROX5 x 1 mL | 200 x 20 µL rxnsPB20.64-05##### Lo-ROX, Hi-ROX or No-ROX? The appropriate ROX concentration depends on the real-time PCR system used. Clara® Probe Mix is therefore available in different reference dye configurations. - No-ROX: Without a passive ROX reference dye - Lo-ROX: Low ROX concentration - Hi-ROX: Hohe ROX concentration - Separate-ROX: ROX is supplied separately and can be dosed flexibly Not sure which ROX variant is right for your instrument? [Ask our qPCR support team.](https://www.nippongenetics.eu/en/contact/) ![Clara® Probe Mix amplification across low template concentrations](https://www.nippongenetics.eu/app/uploads/dynamic/clara-probe-mix-low-template-detection-120x0-c-default.jpg) ##### Sensitive detection even at low template concentrations Clara® Probe Mix enables reproducible amplification across a broad range of template concentrations and supports reliable detection even at low template levels. Experimental detailsAmplification of common housekeeping genes (GAPDH, and β-2-Microglobulin \[B2M\]) using Clara® Probe Mix (pink curves) or TaqMan Fast Advanced Master Mix (grey curves). Amplification curves are shown in the left panels and efficiency in the right panels. Three (for GAPDH) or four (for B2M) serial dilutions of mouse cDNA template were used, corresponding to 1 ng/μL, 100 pg/μL, and 10 pg/μL, or 1 ng/μL, 100 pg/μL, 10 pg/μL, and 1 pg/μL, respectively. The reaction volume was 20 μL. The cycling conditions were: 95 °C 2 min, followed by 50 cycles of 95 °C 10 s, 60 °C 30 s. Clara® Probe Mix overperforms TaqMan Fast Advanced Master Mix, showing high sensitivity and reproducible amplification even at low template concentrations with optimal efficiency. Data provided by PCR Biosystems. ![Clara® Probe Mix 6-plex qPCR performance on FastGene® qFYR](https://www.nippongenetics.eu/app/uploads/dynamic/clara-probe-mix-fastgene-qfyr-6plex-120x0-c-default.jpg) ##### Multiplex qPCR on the [FastGene® qFYR Real-Time PCR System](https://www.nippongenetics.eu/en/products/lab-instruments/qpcr-thermal-cycler/fastgene-qfyr-plus-real-time-pcr-plus-system/) **Six targets. One reaction.** Clara® Probe Mix has been evaluated in a 6-plex assay using the FastGene® qFYR Real-Time PCR System. All six targets were successfully amplified within a single reaction. [**Discover the FastGene® qFYR Real-Tome PCR System.**](https://www.nippongenetics.eu/en/products/lab-instruments/qpcr-thermal-cycler/fastgene-qfyr-plus-real-time-pcr-plus-system/) Experimental detailsClara® Probe Mix was used in a 6-plex reaction on a FastGene® qFYR Real-Time PCR System. Two technical replicates were used for each target. All 6 targets were successfully amplified in a single well in both replicates. ### Specifications **Parameter** **Clara® Probe Mix**ApplicationProbe-based qPCRConcentration4xTemplateDNA / cDNAPolymeraseHot start Taq DNA polymeraseHot start mechanismAntibody-mediatedSingleplexYesMultiplexYesCyclingStandard & FastReaction volume20 µLROX optionsNo-ROX | Lo-ROX | Hi-ROX | Separate-ROXStorage−30 to −20 °C ### Downloads ##### Manuals [Clara Probe Mix Lo-ROX | PB20.61 | Manual](https://www.nippongenetics.eu/app/uploads/pb20-61-clara-probe-mix-lo-rox-manual.pdf) [Clara Probe Mix Hi-ROX | PB20.62 | Manual](https://www.nippongenetics.eu/app/uploads/pb20-62-clara-probe-mix-hi-rox-manual.pdf) [Clara Probe Mix No-ROX | PB20.63 | Manual](https://www.nippongenetics.eu/app/uploads/pb20-63-clara-probe-mix-no-rox-manual.pdf) [Clara Probe Mix Separate-ROX | PB20.64 | Manual](https://www.nippongenetics.eu/app/uploads/pb20-64-clara-probe-mix-separate-rox-manual.pdf) ##### Flyer [Clara Probe Mix | PB20.61-64 | Product Flyer](https://www.nippongenetics.eu/app/uploads/clara-probe-mix-flyer.pdf) ##### Safety Data Sheet [Clara Probe Mix Lo-ROX | PB20.61 | Safety Data Sheet](https://www.nippongenetics.eu/app/uploads/pb20-61-clara-probe-mix-lo-rox-msds.pdf) [Clara Probe Mix Hi-ROX | PB20.62 | Safety Data Sheet](https://www.nippongenetics.eu/app/uploads/pb20-62-clara-probe-mix-hi-rox-msds.pdf) [Clara Probe Mix No-ROX | PB20.63 | Safety Data Sheet](https://www.nippongenetics.eu/app/uploads/pb20-63-clara-probe-mix-no-rox-msds.pdf) [Clara Probe Mix Separate-ROX | PB20.64 | Safety Data Sheet](https://www.nippongenetics.eu/app/uploads/pb20-64-clara-probe-mix-separate-rox-msds.pdf) ### Free sample ##### Not sure if Clara® Probe Mix is right for your assay? ###### Try a free sample. Evaluate Clara® Probe Mix using your own template, assay and qPCR instrument. Request a free sample and compare sensitivity, reproducibility and multiplex performance directly with your current qPCR master mix. ### Reviews There are no reviews yet. Only logged in customers may leave a review.