IC Green qPCR Universal Kit
It is well-known that SYBR Green is extensively inhibiting the qPCR. This fact led to the development of SYBR resistant enzymes. An alternative approach is to develop a dye that does not inhibit the reaction. This dye is named FastGene® IC Green. FastGene® IC Green is an intercalating dye, only detecting double stranded DNA. By not inhibiting the reaction, the FastGene® IC Green kit is able to detect genes at a lower CT-value, creating a higher sensitivity!
- Quantification of Gene Expression
- Quantification of Gene Copy number
- Melt-curve analysis
- Detection of Gene Expression (Knock-out analysis)
No inhibition – For the highest sensitivity
The superior buffer chemistry enables the detection of low copy number genes, which with other dyes could not be detected. The comparison to competitors shows that FastGene® IC Green is one of the best qPCR mixes available. This has been confirmed by customers analyzing many different genes.
Robust chemistry for faster results
The FastGene® IC Green buffers were designed to have a superior robustness. This guarantees the linearity of the qPCR and creates a better accuracy, essential for reproducible results. Additionally, qPCRs can be performed at lower amplifcation times for example using fast protocols.
Universal – for any qPCR instrument
The FastGene® IC Green kit is universal. The reference dyes come in a separate vial and can be added once to the master mixes. Hence, this kit can be used with qPCR instruments which need a high ROX™ concentration as well as instruments that need a low concentration or no ROX™. There is even a special version with fluorescein.
FastGene® IC Green Universal is available in three different package units:
- 1 x 1 mL | 100 x 20 µL reactions | Cat.No. LS4001
- 5 x 1 mL | 500 x 20 µL reactions | Cat.No. LS4005
- 1 x 50 mL | 5000 x 20 µL reactions | Cat.No. LS4050
Dr. G. Efstathios, Assoc. Professor, Food Science and Nutrition, University of the Aegean, Greece –
I have used the FastGene IC Green 2X qPCR Universal Mix (LOW ROX) in parallel and in comparison with another commercial kit provided by the manufacturer of my instrument. The kit of Nippon Genetics performed very well returning for the same cDNA quantities significant lower Ct values and higher ΔRn differences compared to that of the competitor. Amplification curves were also very nice with adequate consistency between the technical replicates. The kit of Nippon Genetics also provided similar amplification efficiencies with that of competitor (2 genes tested with cDNAs ranging from 100 to 0.01 ng). I will surely work with it!