# FastGene Scriptase Basic cDNA Kit --- url: https://www.nippongenetics.eu/en/products/pcr-reagents-and-enzymes/reverse-transcriptase/cdna-synthesis-kits/fastgene-scriptase-basic-20000-u/ language: en type: product modified: 2026-01-05T10:01:06+01:00 --- ## Kit for reverse transcription ## Product Details - **SKU**: LS62 - **Stock status**: instock - **Categories**: PCR Reagents and Enzymes, Reverse Transcription, cDNA Synthesis Kits - **Tags**: High RNA concentrations, Optimized MuLV Transcriptase, Robust ## Attributes - **Template**: RNA - **Technique**: Reverse transcription 20,000 units at 200 U/µL (for 100 reactions) | With buffer, dNTP mix and sterile water | With Oligo dTs, random hexamers and RNases inhibitor | Endonuclease-free, exonuclease-free, RNase-free, inhibitor-free - 20,000 units at 200 U/µL (for 100 reactions) - Buffer (10x FastGene® Scriptase Basic Buffer), dNTP mixture (2 mM each) and sterile water (RNase-free) included - Oligo dTs, random hexamers and RNases inhibitor included (not included in [FastGene® Scriptase Basic, LS52](https://www.nippongenetics.eu/en/products/pcr-reagents-and-enzymes/enzymes/reverse-transcriptase/reverse-transcriptase-reverse-transcriptase/fastgene-scriptase-basic-20000-u-2/)) - Endonuclease-free, exonuclease-free, RNase-free, inhibitor-free - Reverse transcriptase for the quantification of gene expression - For high DNA concentrations ### Details ##### Optimized for better performance The FastGene® Scriptase Basic is an enhanced version of the Murine Leukemia Virus (MuLV) reverse transcriptase. Hence, like the wildtype, it has the ability to synthesize a cDNA strand, a reduced RNase H activity and processitivity. The robustness however was greatly increased. It is the perfect enzyme for large RNA quantities and easy templates. ##### Applications - Quantification of Gene Expression - Endpoint RT-PCR - High RNA concentrations ##### No inhibition – Even at large concentration The special buffer formulation permits a high RNA concentration. Other reverse transcriptases are not able to process such large quantities. ##### Designed for Endpoint RT-PCR The FastGene® Scriptase Basic was designed for large RNA quantities, typically used in an endpoint RT-PCR. Nonetheless, it is also able to process lower RNA concentrations. ##### Enzyme only or cDNA Synthesis kit The FastGene® Scriptase Basic is available as enzyme only ([FastGene® Scriptase Basic, LS52](https://www.nippongenetics.eu/en/products/pcr-reagents-and-enzymes/enzymes/reverse-transcriptase/reverse-transcriptase-reverse-transcriptase/fastgene-scriptase-basic-20000-u-2/)), containing the enzyme, buffer and dNTPs as well as a cDNA synthesis kit which contains the components of the enzyme plus Oligo dTs, random hexamers and RNase inhibitor (LS62). We have also chosen a 100 reactions kit size so that you have enough for a whole 96-well plate. ##### Comparison of FastGene® Scriptase Basic and a conventional MuLV (A) Competitor N (B) Scriptase Basic FastGene® ![](https://www.nippongenetics.eu/app/uploads/2017/04/Data-1-300x71.png) RNA (ng) 2 1 0.5 0.1 0.01 2 1 0.5 0.1 0.01 Fig. 1: Higher sensitivity of the FastGene® Scriptase Basic. Compared to a wildtype MuLV (A) the FastGene® Scriptase Basic (B) is an optimized MuLV-Reverse transcriptase which has a higher sensitivity, being able to produce a template from RNA concentrations as low as 0.1 ng. ### Documents [LS-62-Scriptase Basic cDNA Manual](https://www.nippongenetics.eu/app/uploads/2018/02/LS-62-Scriptase-Basic-cDNA-Manual.pdf) [MSDS](https://www.nippongenetics.eu/app/uploads/2019/11/LS62_cDNA-synthesis-kit_SDS_eng.pdf) ### FAQ ##### I am using your Scriptase Basic ([LS52](https://www.nippongenetics.eu/en/products/pcr-reagents-and-enzymes/enzymes/reverse-transcriptase/reverse-transcriptase-reverse-transcriptase/fastgene-scriptase-basic-20000-u-2/)) and I wanted to know if I can use this enzyme in combination with a DNA removal kit which contains a DNase Stop buffer with 20 mM EGTA? All DNA/RNA working enzymes need metal ions like Mg2+, Ca2+, Mn2+ and so on. DNase I requires Mg2+ or Ca2+ as well. Therefore, DNase I Stop buffer must contain 20 mM of EGTA or EDTA (as metal chelating agent) to trap the metal ions. For most enzymes the working condition is around 1~10 mM. So, 20 mM of EGTA is enough to stop both reactions, DNase treatment and RT. To keep Scriptase Basic (LS52) working further on, the concentration of Mg2+ has to be increased to make the final Mg2+ concentration back to the working concentration of the enzyme. But in this case the DNase I will start again and degrade DNA/cDNA. This is a common problem with all Reverse Transcriptases. ##### What is the optimal temperature range for Scriptase Basic and Scriptase II? The optimal temperature range for the Scriptases is 42°C to 50°C. A temperature above 50°C is not recommended. ##### Can Scriptase Basic and Scriptase II be used for NGS applications? As the Scriptase Basic and Scriptase II are for ssDNA only, they are not suitable for NGS applications. ### Rated **5** out of 5 based on 2 customer ratings 2 reviews for FastGene Scriptase Basic cDNA Kit 1. ![](https://secure.gravatar.com/avatar/045ce081e26bebc864f36cdc8b90d3771382900ae4d1710e61b0d5dfab3a693d?s=60&d=mm&r=g)Rated **5** out of 5 **Dott.ssa S. Marchianò, PhD Perugia University Department of Medicine and Surgery, Italy** – 24.10.2025 We are using Fastgene scriptase Basic cDNA kit cat# LS62 from some years and we are really satisfied. It is our first choice as we can obtain very good quantities of cDNA starting from a quantity of RNA till 4 ug in each 20ul reaction. [Verified User](https://www.nippongenetics.eu/en/authenticity-of-reviews/) 2. ![](https://secure.gravatar.com/avatar/618be5cfda3638542583770ec97353b135323366804d0ce08866e4407dc0c4e3?s=60&d=mm&r=g)Rated **5** out of 5 **UNAm** – 22.09.2017 Fácil de usar y con muy buenos resultados, además de un muy buen precio. Translated by NIPPON Genetics EUROPE: It is easy to use and leads to very nice results, combined with a very good price. [Verified User](https://www.nippongenetics.eu/en/authenticity-of-reviews/) Only logged in customers may leave a review.