# FastGene Scriptase Basic --- url: https://www.nippongenetics.eu/en/products/pcr-reagents-and-enzymes/reverse-transcriptase/reverse-transcriptase-reverse-transcriptase/fastgene-scriptase-basic-20000-u-2/ language: en type: product modified: 2026-09-22T12:16:25+02:00 --- ## Reverse transcriptase ## Product Details - **SKU**: LS52 - **On sale**: yes - **Stock status**: instock - **Categories**: PCR Reagents and Enzymes, Reverse Transcription, Reverse Transcriptase - **Tags**: High RNA concentrations, Optimized MuLV Transcriptase, Robust ## Attributes - **Template**: RNA - **Technique**: Reverse transcription 20,000 units at 200 U/µL (for 100 reactions) | Buffer (10x FastGene® Scriptase Basic Buffer), dNTP mixture (2 mM each) and sterile water (RNase-free) included | Endonuclease-free, exonuclease-free, RNase-free and inhibitor-free - 20,000 units at 200 U/µL (for 100 reactions) - Buffer (10x FastGene® Scriptase Basic Buffer), dNTP mixture (2 mM each) and sterile water (RNase-free) included - Endonuclease-free, exonuclease-free and RNase-free - Reverse transcriptase for the quantification of gene expression - For high DNA concentrations ### Details ##### Optimized for better performance The FastGene® Scriptase Basic is an enhanced version of the Murine Leukemia Virus (MuLV) reverse transcriptase. Hence, like the wildtype, it has the ability to synthesize a cDNA strand, a reduced RNase H activity and processitivity. The robustness however was greatly increased. It is the perfect enzyme for large RNA quantities and easy templates. ##### Applications - Quantification of ggene expression - Endpoint RT-PCR - High RNA concentrations ##### No inhibition – Even at large concentration The special buffer formulation permits a high RNA concentration. Other reverse transcriptases are not able to process such large quantities. ##### Designed for Endpoint RT-PCR The FastGene® Scriptase Basic was designed for large RNA quantities, typically used in an endpoint RT-PCR. Nonetheless, it is also able to process lower RNA concentrations. ##### Enzyme only or cDNA Synthesis kit The FastGene® Scriptase Basic is available as enzyme only (LS52), containing the enzyme, buffer and dNTPs as well as a cDNA synthesis kit ([FastGene® Scriptase Basic cDNA Kit](https://www.nippongenetics.eu/en/products/pcr-reagents-and-enzymes/enzymes/reverse-transcriptase/reverse-transcriptase-reverse-transcriptase/fastgene-scriptase-basic-20000-u/)) which contains the components of the enzyme plus: - Oligo dTs - Random hexamers - RNase inhibitor. We have also chosen a 100 reactions kit size so that you have enough for a whole 96-well plate. ##### Comparison of FastGene® Scriptase Basic and a conventional MuLV (A) Competitor N (B) FastGene® Scriptase Basic ![](https://www.nippongenetics.eu/app/uploads/2017/04/Data-1-300x71.png) RNA (ng) 2 1 0,5 0,1 0,01 2 1 0,5 0,1 0,01 Fig. 1: Higher sensitivity of the FastGene® Scriptase Basic. Compared to a wildtype MuLV (A) the FastGene® Scriptase Basic (B) is an optimized MuLV-Reverse transcriptase which has a higher sensitivity, being able to produce a template from RNA concentrations as low as 0.1 ng. ### FAQ ##### I am using your Scriptase Basic (LS52) and I wanted to know if I can use this enzyme in combination with a DNA removal kit which contains a DNase Stop buffer with 20 mM EGTA? All DNA/RNA working enzymes need metal ions like Mg2+, Ca2+, Mn2+ and so on. DNase I requires Mg2+ or Ca2+ as well. Therefore, DNase I Stop buffer must contain 20 mM of EGTA or EDTA (as metal chelating agent) to trap the metal ions. For most enzymes the working condition is around 1~10 mM. So, 20 mM of EGTA is enough to stop both reactions, DNase treatment and RT. To keep Scriptase Basic (LS52) working further on, the concentration of Mg2+ has to be increased to make the final Mg2+ concentration back to the working concentration of the enzyme. But in this case the DNase I will start again and degrade DNA/cDNA. This is a common problem with all Reverse Transcriptases. ##### What is the optimal temperature range for Scriptase Basic and Scriptase II? The optimal temperature range for the Scriptases is 42°C to 50°C. A temperature above 50°C is not recommended. ##### Can Scriptase Basic and Scriptase II be used for NGS applications? As the Scriptase Basic and Scriptase II are for ssDNA only, they are not suitable for NGS applications. ### Documents ##### Manual [Manual | FastGene Scriptase Basic | LS52](https://www.nippongenetics.eu/app/uploads/manual-LS52-FastGene-Scriptase-Basic.pdf) ##### MSDS [MSDS | FastGene Scriptase Basic | LS52](https://www.nippongenetics.eu/app/uploads/msds-fastgene-scriptase-basic-ls52.pdf) ##### Application Note [Application Note LS52](https://www.nippongenetics.eu/app/uploads/2019/11/LS52_FG-Scriptase-Basic_AN_2018_20.pdf) ### Reviews There are no reviews yet. Only logged in customers may leave a review.