PCRBIO UltraScript 2.0 Reverse Transcriptase

Reverse transcriptase

  • Highly thermostable: Reverse transcription of templates at 37-65°C and higher
  • Ready in 10-30 minutes
  • High yields of cDNA from minimal amounts of RNA
  • Reduced RNase H activity, including RNase Inhibitor
  • Enzyme with buffer
Price on request


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Cat. No. N/A
PCRBIO UltraScript Reverse Transcriptase (PB30.33-01)
PCRBIO UltraScript Reverse Transcriptase - Wide temperature range

Details

UltraScript 2.0 Reverse Transcriptase is a highly thermostable reverse transcriptase engineered for superior cDNA synthesis speed, yield and representation of the most challenging RNA sample types.

This modified MMLV reverse transcriptase can be used with reaction temperatures of over 55°C, giving improved specificity, higher cDNA yields and more full length cDNA product. 

Features
  • Highly thermostable reverse transcriptase 55°C to 65°C and above
  • Advanced RNase inhibitor
  • High cDNA yields from as little as 20pg total RNA
  • Accurate reverse transcription of GC-rich and highly structured transcripts
  • High sensitivity for challenging templates
  • Reduced RNase H activity
  • Available as a stand-alone enzyme with buffer, a cDNA synthesis kit with premixed oligos and a cDNA synthesis kit with separate oligos
Components
  • UltraScript 2.0 (200 units/µL) with RNase inhibitor
  • 5x UltraScript Buffer
Applications
  • cDNA synthesis for PCR analysis, cloning, cDNA library preparation and Next Generation Sequencing
  • Viral RNA targets
  • miRNA targets
  • Efficient synthesis from total RNA or poly(A)+ RNA

The enhanced thermostability of UltraScript 2.0 Reverse Transcriptase (RTase) allows reaction temperatures of over 55°C to be used, giving improved specificity, higher cDNA yields, and more full length cDNA product. The enzyme remains partially active even up to 90°C and is designed for efficient reverse transcription of the most difficult RNA templates, including GC-rich and highly structured transcripts. 

UltraScript 2.0 RTase is designed for efficient cDNA synthesis from a broad range of RNA concentrations. UltraScript 2.0 kits can be used with 20 pg to 3.5 μg total RNA or oligo(dT) purified mRNA. The RTase is blended with an advanced RNase inhibitor preventing degradation of RNA by contaminating RNase.

Specifications

UltraScript 2.0 RTase is available in three convenient formats, each with dNTPs, MgCl2, and RNase inhibitor included.

  • Our stand-alone enzyme with 5x buffer (PB30.33-01/04) gives users the flexibility to define their own priming strategy and offers exceptional performance with gene-specific primers, oligo(dT) and random hexamers.
UltraScript 2.0 cDNA Reverse Transcriptase (PB30.33) 10 000 U 40 000 U
UltraScript 2.0 (200 units/µL) with RNase inhibitor 2 x 25 µL 2 x 100 µL
5x UltraScript Buffer 1  x 200 µL 4 x 200 µL
  • The 2-tube UltraScript 2.0 cDNA Synthesis Kit contains all the components required for fast, reliable and unbiased cDNA synthesis, including an optimised blend of anchored oligo(dT) and random hexamers.
UltraScript 2.0 cDNA Synthesis Kit (PB30.31-10 – more information) 100 rxn
UltraScript 2.0 (200 units/µL) with RNase inhibitor 1 x 100 µL
5x cDNA Synthesis Mix 4 x 100 µL
  • UltraScript 2.0 cDNA Synthesis Kit Separate Oligos provides anchored oligo(dT) and random hexamers in separate tubes, for user optimisation depending on the type of analysis needed.
UltraScript 2.0 cDNA Synthesis Kit Separate Oligos (PB30.32-10 – more information) 100 rxn
UltraScript 2.0 (200 units/µL) with RNase inhibitor 1 x 100 µL
5x UltraScript Buffer 2 x 200 µL
100 μM Anchored Oligo(dT)18 1 x 100 µL
100 μM Random Hexamers 1 x 100 µL

Data

Wide temperature range

PCRBIO UltraScript Reverse Transcriptase - Wide temperature range - Data

UltraScript 2.0 Reverse Transcriptase gives similar amounts of product across a wide range of temperatures in endpoint RT-PCR. Mouse reference RNA was reverse transcribes using UltraScriopt 2.0 Reverse Transcriptase. G-Act cDNA was amplified using qPCRBIO SyGreen Mix and visualised on 1% agarose gel.

 

Downloads

Manual

Manual – PCRBIO UltraScript 2.0 Reverse Transcriptase (PB30.33)

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