JustBlue Prestained Protein Marker

Single coloured protein ladder (10 - 180 kDA)

  • Broad range: 10 – 180 kDa
  • 11 single coloured bands
  • 2 reference bands with higher intensity at 28 kDa and 72 kDa
  • Ready to use: loading buffer included
  • Sharp bands
  • 500 µL: for 100 mini or 50 large gels
Price on request


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Cat. No. MWP05 0.5 mL
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Details

JustBlue Prestained Protein Marker

The JustBlue Protein Marker is a reasonable priced alternative to the BlueStar Marker. Eleven proteins are coupled with a blue chromophor. This Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon or nitrocellulose) and for approximating the size of proteins. We recommand 4-5 µl per well for protein electrophoresis and 2-3 µl per well for Western Transfer.

 

 

  • Broad range: 10 – 180 kDa
  • High performance: 11 bands with 1 colour
  • Ready to use: Supplied in a loading buffer for direct loading on gels
  • Reference bands: 28 kDa  and 72 kDa with higher intensity
  • Sharp bands
  • 1x 500 µL Protein Marker, sufficient for 100 mini or 50 large gels
Storage

Stable at -20°C for 12 months.
Stable at +4°C for 3 months.

Applications

• Monitoring of protein migration during SDS-polyacrylamide gel electrophoresis

• Monitoring of protein transfer onto membranes during Western blotting

• Sizing of proteins on SDS-polyacrylamide gels and Western blots

Documents

Manual

MWP05_Manual

Safety Data Sheet (MSDS)

MWP05_MSDS

FAQ

Could you send me information about the concentration of each individual band of the JustBlue Prestained Protein Marker. This because someone wants to use it to estimate protein amount of his samples. The only info I have is that the concentration is 0.1 to 0.5 mg/ml. Does that mean 0.1mg/ml for normal bands and 0.5mg/ml for the 25kDa and 75kDa bands?

Unfortunately, in general it is not possible to use dyed protein standards in order to estimate the amount of protein sample. Since the dyes used to couple with the proteins of the ladder are different from the Coomassie Blue dye used to stain the protein(s) of interest, it’d help your customer to obtain a more accurate molecular weight estimation by using an unstained protein ladder because both the unstained protein ladder and the target protein(s) are then stained with the Coomassie Blue dye.

Does this marker contain Formaldehyde or Formamide?

No.

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