The FastGene® T4 DNA Ligase catalyzes the formation of a covalent bond between the 5′-phosphate and 3′-OH in nicked duplex DNA or at two DNA ends. This activity is very useful to ligate DNA fragments with either cohesive or blunt ends, that are generated by restriction enzyme digestion. T4 DNA Ligase can also ligate RNA with DNA or RNA in a double helix with low efficiency. The T4 DNA Ligase is cloned and expressed in E. coli, and purified to homogeneity. The Ligase is free of endonuclease, exonuclease and phosphatase.
- Molecular weight: 55 kDa
- Reaction temperature: Room temperature
- Heat inactivation: 65℃ for 10 min
- Cloning of restricted DNA fragments into a plasmid
- Addition of linker or adaptor DNAs to cohesive or blund-ended DNA