FastGene T4 DNA Ligase

Connection of DNA strands

  • Catalyses the formation of a phosphodiester bond for the connection of two DNA strands
  • T4 Ligase will join blunt ends and sticky ends
  • Supplied with optimal buffer conditions for an efficient ligation
  • Highest activity and purity 
Price on request


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Cat. No. FG-T4
Ligases

Details

The FastGene® T4 DNA Ligase catalyzes the formation of a covalent bond between the 5′-phosphate and 3′-OH in nicked duplex DNA or at two DNA ends. This activity is very useful to ligate DNA fragments with either cohesive or blunt ends, that are generated by restriction enzyme digestion. T4 DNA Ligase can also ligate RNA with DNA or RNA in a double helix with low efficiency. The T4 DNA Ligase is cloned and expressed in E. coli, and purified to homogeneity. The Ligase is free of endonuclease, exonuclease and phosphatase.

Characteristics
  • Molecular weight: 55 kDa
  • Reaction temperature: Room temperature
  • Heat inactivation: 65℃ for 10 min
Applications
  • Cloning of restricted DNA fragments into a plasmid
  • Addition of linker or adaptor DNAs to cohesive or blund-ended DNA

Downloads

Manual

Manual_FG-T4_T4 DNA Ligase

Product inquiry

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