FastGene® Plasmid Mini Kits are designed for rapid small scale isolation and purification of high copy and low copy plasmid DNA. The ready-to-use plasmid DNA is of high quality in low-salt Tris buffer and suitable for typical downstream applications: Cloning, sequencing, PCR, transformation and restriction analysis.
One kit with all components
Each kit comes with ready-to-use LB-Broth capsules. Add one LB-Broth capsule in 40 mL water, autoclave your solution and
start your cloning experiment. The kit includes everything that is needed for a plasmid preparation.
High and low-copy plasmid DNA preparation
The FastGene® Plasmid Mini Kit is designed for rapid small scale isolation and purification of high copy and low copy plasmid DNA. The purified plasmid DNA is of high quality and ready to use in low-salt Tris buffer, suitable for typical downstream applications: Cloning, sequencing, PCR, transformation and restriction analysis.
High yield – High purity – Fast preparation
The FastGene® Plasmid Mini Kits are faster than competitors with comparable yield. This allows you to save time and perform your downstream application quicker.
The gel demonstrates that FastGene® Plasmid Mini Kits yield equal amount of plasmid DNA compared to other supplier with even more time consuming protocols. The preparation with the FastGene® Plasmid Mini Kit was performed by using the Fast Protocol.
Fig. 1: pBluescript plasmid DNA was isolated from a 1,4 ml E.coli culture (DH5α in LB medium) according to the recommended procedures of different kits and eluted in 50 µl elution buffer. 2 µl of each eluate were loaded on a 0,7% TAE agarose gel.
Fig.2: Typical fluorescent capillary sequencing result from pBluescript plasmid DNA purified using the FastGene® Plasmid Mini Kit. Cycle sequencing reaction was performed on an ABI3100 sequenzer. 1 µl of purified plasmid DNA was used as a template. Dye terminators were removed by FastGene® Dye Terminator Removal Kit