Optimized for better performance
The FastGene® Scriptase Basic is an enhanced version of the Murine Leukemia Virus (MuLV) reverse transcriptase. Hence, like the wildtype, it has the ability to synthesize a cDNA strand, a reduced RNase H activity and processitivity. The robustness however was greatly increased. It is the perfect enzyme for large RNA quantities and easy templates.
- Quantification of Gene Expression
- Endpoint RT-PCR
- High RNA concentrations
No inhibition – Even at large concentration
The special buffer formulation permits a high RNA concentration. Other reverse transcriptases are not able to process such large quantities.
Designed for Endpoint RT-PCR
The FastGene® Scriptase Basic was designed for large RNA quantities, typically used in an endpoint RT-PCR. Nonetheless, it is also able to process lower RNA concentrations.
Enzyme only or cDNA Synthesis kit
The FastGene® Scriptase Basic is available as enzyme only (FastGene® Scriptase Basic, LS52), containing the enzyme, buffer and dNTPs as well as a cDNA synthesis kit which contains the components of the enzyme plus Oligo dTs, random hexamers and RNase inhibitor (LS62). We have also chosen a 100 reactions kit size so that you have enough for a whole 96-well plate.
Comparison of FastGene® Scriptase Basic and a conventional MuLV
(A) Competitor N (B) Scriptase Basic FastGene®
RNA (ng) 2 1 0.5 0.1 0.01 2 1 0.5 0.1 0.01
Fig. 1: Higher sensitivity of the FastGene® Scriptase Basic.
Compared to a wildtype MuLV (A) the FastGene® Scriptase Basic (B) is an optimized MuLV-Reverse transcriptase which has a higher sensitivity, being able to produce a template from RNA concentrations as low as 0.1 ng.