FastGene Scriptase Basic cDNA Kit

Ligation of DNA strands

  • 20,000 units at 200 U/µL (for 100 reactions)
  • Buffer (10x FastGene® Scriptase Basic Buffer), dNTP mixture (2 mM each) and sterile water (RNase-free) included
  • Oligo dTs, random hexamers and RNases inhibitor included (not included in FastGene® Scriptase Basic, LS52)
  • Endonuclease-free, exonuclease-free, RNase-free, inhibitor-free
  • Reverse transcriptase for the quantification of gene expression
  • For high DNA concentrations
Price on request


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Cat. No. LS62
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Details

Optimized for better performance

The FastGene® Scriptase Basic is an enhanced version of the Murine Leukemia Virus (MuLV) reverse transcriptase. Hence, like the wildtype, it has the ability to synthesize a cDNA strand, a reduced RNase H activity and processitivity. The robustness however was greatly increased. It is the perfect enzyme for large RNA quantities and easy templates.

Applications
  • Quantification of Gene Expression
  • Endpoint RT-PCR
  • High RNA concentrations
No inhibition – Even at large concentration

The special buffer formulation permits a high RNA concentration. Other reverse transcriptases are not able to process such large quantities.

Designed for Endpoint RT-PCR

The FastGene® Scriptase Basic was designed for large RNA quantities, typically used in an endpoint RT-PCR. Nonetheless, it is also able to process lower RNA concentrations.

Enzyme only or cDNA Synthesis kit

The FastGene® Scriptase Basic is available as enzyme only (FastGene® Scriptase Basic, LS52), containing the enzyme, buffer and dNTPs as well as a cDNA synthesis kit which contains the components of the enzyme plus Oligo dTs, random hexamers and RNase inhibitor (LS62). We have also chosen a 100 reactions kit size so that you have enough for a whole 96-well plate.

Comparison of FastGene® Scriptase Basic and a conventional MuLV

(A) Competitor N                                       (B) Scriptase Basic FastGene®

RNA (ng)   2      1     0.5     0.1    0.01                        2       1      0.5     0.1    0.01

Fig. 1: Higher sensitivity of the FastGene® Scriptase Basic.
Compared to a wildtype MuLV (A) the FastGene® Scriptase Basic (B) is an optimized MuLV-Reverse transcriptase which has a higher sensitivity, being able to produce a template from RNA concentrations as low as 0.1 ng.

Documents

LS-62-Scriptase Basic cDNA Manual
MSDS

FAQ

I am using your Scriptase Basic (LS52) and I wanted to know if I can use this enzyme in combination with a DNA removal kit which contains a DNase Stop buffer with 20 mM EGTA?

All DNA/RNA working enzymes need metal ions like Mg2+, Ca2+, Mn2+ and so on. DNase I requires Mg2+ or Ca2+ as well. Therefore, DNase I Stop buffer must contain 20 mM of EGTA or EDTA (as metal chelating agent) to trap the metal ions.  For most enzymes the working condition is around 1~10 mM. So, 20 mM of EGTA is enough to stop both reactions, DNase treatment and RT.

To keep Scriptase Basic (LS52) working further on, the concentration of Mg2+ has to be increased to make the final Mg2+ concentration back to the working concentration of the enzyme. But in this case the DNase I will start again and degrade DNA/cDNA.

This is a common problem with all Reverse Transcriptases.

What is the optimal temperature range for Scriptase Basic and Scriptase II?

The optimal temperature range for the Scriptases is 42°C to 50°C. A temperature above 50°C is not recommended.

Can Scriptase Basic and Scriptase II be used for NGS applications?

As the Scriptase Basic and Scriptase II are for ssDNA only, they are not suitable for NGS applications.

1 review for FastGene Scriptase Basic cDNA Kit

  1. UNAm

    Fácil de usar y con muy buenos resultados, además de un muy buen precio.

    Translated by Nippon Genetics:

    “It is easy to use and leads to very nice results, combined with a very good price.”

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