Ava I

Restriction Enzyme

Recognition Sequence	Amount
C/YCGRG GRGCY/C	2000 Units, 10 Units/µL

Overhang	CpG Methylation
5′ YCGR	Sensitive

Incubation Temp.	Heat Inactivation
37°C	80°C for 20 min

Activity in FastGene Buffer

Buffer I	Buffer II	Buffer III	Buffer IV	FastCut Buffer
25%	100%	100%	100%	100%

Supplied Buffer	Enzyme Type
Buffer IV	Type IIP

Isoschizomers
Ama87I, BmeT110I, BsiHKCI, BsoBI, Eco88I

Cat. No. FG-AvaI

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