PluT I

Restriction Enzyme

Recognition Sequence	Amount
GGCGC/C C/CGCGG	500 Units, 10 Units/µL

Overhang	CpG Methylation
3′ GCGC	Sensitive

Incubation Temp.	Heat Inactivation
37°C	65°C for 20 min

Activity in FastGene Buffer

Buffer I	Buffer II	Buffer III	Buffer IV	FastCut Buffer
75%	25%	10%	100%	100%

Supplied Buffer	Enzyme Type
Buffer IV	Type IIF,P

Isoschizomers
DinI, EgeI, EheI, KasI, SfoI

Cat. No. FG-PluTI

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