Sph I

Enzyme de restriction

Recognition Sequence	Amount
GCATG/C C/GTACG	600 Units, 5 Units/µL

Overhang	CpG Methylation
3′ CATG	Not sensitive

Incubation Temp.	Heat Inactivation
37°C	65°C for 20 min

Activity in FastGene Buffer

Buffer I	Buffer II	Buffer III	Buffer IV	FastCut Buffer
50%	100%	50%	75%	100%

Supplied Buffer	Enzyme Type
Buffer II	Type IIP

Isoschizomer
PaeI

Cat. No. FG-SphI

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