FastGene Dye Terminator Removal Kit

Removal of dyes

 

 

Price on request


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Cat. No. FG-9411 50 preps

Details

FastGene® Dye Terminator Removal Kit removes dye terminator molecules from sequencing samples using an efficient and reasonably priced gel filtration. The Kit includes a bottle of gelfiltration matrix, resuspension buffer and filter spin columns. At first an aliquot of the matrix will be mixed with resuspension buffer. Thereafter the equilibrated matrix is transferred into filter spin columns. After a brief spin, up to 20 µl of the sequencing reaction can be loaded onto the column. The last centrifugation step will elute the purified sample. All kit components can be stored at room temperature.

Applications

Application data

The DNA purified by the FastGene® Dye Terminator Removal Kit shows a very good performance in sequencing experiments, no dye blobs occur.

Fig. 1: 20 µl sequencing reaction including 2,5 µl BigDye Terminator v3.1 was purified with FastGene® Dye Terminator Removal Kit and analyzed on an ABI 3100 sequencer.

Specifications

Parameter Dye Terminator Removal
Max. sample volume 20 µl sequencing reaction
Recovery > 90%
Prep time 5 minutes
Store at room temperature

Protocol

Documents

FG-9411_FG-Dye-Terminator-Removal-Kit_Manual-1
FG-9411_FG-Dye-Terminator-Removal-Kit_QuickGuide

1 review for FastGene Dye Terminator Removal Kit

  1. Igor Kondrychyn, RIKEN CBD

    Fast and easy to use. Before injection, we dry the purified samples with SpeedVac (without heating) and resuspend in HiDi formamide.

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