Easy enrichment of iRNA
Small RNA molecules can be purified using this addition to our regular RNA purification kit. RNA with miRNA or only miRNA can be purified, depending on your needs.
The FastGene® miRNA Enhancer allows the binding of small RNA to the column of your standard RNA purification protocol. With a single additional step you just have to add the RNA enhancer solution during the purification. Dependent on the kit or protocol you are using, you obtain pure miRNA or total RNA + miRNA. Use the miRNA Enhancer togehter with our FastGene® RNA Premium Kit and you will get pure miRNA.
Or use the FastGene® RNA Basic Kits and you will obtain total RNA together with miRNA. The miRNA Enhancer is also compatible with other RNA extraction kit.
Figure 1: Use the FastGene® miRNA Enhancer with the FastGene® RNA Basic Kit or FastGene® RNA Premium Kit for the recovery of small RNA.
(Lane 1-3) Purification of total RNA + miRNA by using the FastGene® miRNA Enhancer together with the FastGene® RNA Basic Kit.
(Lane 4-6) Purification of RNA by using only the FastGene® RNA Basic Kit, without the FastGene® miRNA Enhancer.
(Lane 7-9) Purification of pure miRNA by using the FastGene® miRNA Enhancer together with the FastGene® RNA Premium Kit.
Tomomi, Faculty for Dental Science, Kyushu University, Japan –
We were able to obtain almost the same results as when using the Kit that we have been using so far.
When using the FastGene RNA Premium Kit and enhancer together, miRNA can be recovered at half the cost so far, so I wanted to shift to this in the future.
(Translated by NIPPON Genetics)