BlueStar Plus Prestained Protein Marker

Three colour protein ladder (10 - 240 kDA)

  • Broad range: 10 – 240 kDa
  • 12 sharp bands with 3 colours (blue, red and green)
  • 2 reference bands: 25 kDa (green) and 75 kDa (red)
  • Ready to use: Supplied in a loading buffer for direct loading
  • 500 µL: for 100 mini or 50 large gels
Price on request


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Cat. No. MWP04 0.5 mL
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Details

BlueStar PLUS Prestained Protein Marker

The BlueStar PLUS Prestained Protein Marker is a three color protein standard with 12 prestained proteins covering a wide range molecular weights from 10 – 240 kDa.
The Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximate sizing of proteins.

MWP04 BlueStar Pprotein Marker map

Contents

Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate, 2 % SDS, 0,2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol).

500 µL Protein Marker sufficient for 100 mini gels or 50 large gels.

Storage

Store at -20°C for 24 months.
Stable at +4°C for 3 months.
Stable at +25°C for 2 weeks.

Recommendations for Loading
  1. Thaw the ladder either at room temperature or at 37-40°C for a few minutes to dissolve precipitated solids. Do not boil.
  2. Mix thoroughly, to ensure that the solution is homogeneous.
  3. Load the following volumes of the ladder on SDS-polyacrylamide gel:
    – 5 μl per well for mini-gels, 3 μl per well for blots;
    – 10 μl per well for large gels, 6 μl per well for blots

Applications

• Monitoring of protein migration during SDS-polyacrylamide gel electrophoresis

• Monitoring of protein transfer onto membranes during Western blotting

• Sizing of proteins on SDS-polyacrylamide gels and Western blots

Documents

Manual

MWP04_Manual

Safety data sheet (MSDS)

FAQ

Which amino acids are linked to the BlueStar Plus Prestained Marker MWP04?

Amino acids involved in the dye reaction are those with amine side chain, such as Arg and Lys.

Can the tryptophane been tagged with the trihalo compounds of the biorad gels or the fluorescent dye?

We do not have information regarding the prestained dye interfering with Bio-rad gel systems or the fluorescent dye associated with it.

Does this marker contain Formaldehyde or Formamide?

No.

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