FastGene Western ECL Kit

Western blot detection kit

Reviews (2)
  • Chemiluminescent substrate based on luminol
  • High sensitivity – Detection of antigens with very low concentrations
  • Long signal duration – Optimal for digital and film-based imaging
  • Fast protocol – 10-30 sec incubation time
  • No optimization required – Easy switch from other brands
  • Perfect for PDVF and nitrocellulose membranes
  • Compatible with Western Blotting Markers
Price on request


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Cat. No. FG-CH01 100 mL

Details

FastGene® Western ECL Kit – Chemiluminescent Western Blot Detection

The FastGene® Western ECL Kit is an enhanced chemiluminescent (ECL) substrate based on luminol.
It is used to detect horseradish peroxidase (HRP) – conjugated secondary antibodies.
The high femtogram or low picogram detection of antigen is enabled by FastGene® Western ECL Kit brilliant sensitivity and long signal duration.
This long chemiluminescent signal duration makes it possible to detect signals on both digital and film-based imaging systems without any loss in signal intensity.
Appropriate primary and secondary antibody dilutions are suggested for attaining optimal signal intensity and duration.

Workflow using FastGene® Western ECL Kit

Mix the luminol solution and peroxide solution in a 1:1 ratio, and thoroughly agitate the chemiluminescent substrate solution well for preparing the 0.1 ml of solution / cm2 of membrane.
Place the membrane with the protein side up and remove the membrane from the chemiluminescent substrate solution. Image the membrane with a digital imager.

Product comparison

Comparison between the FastGene® Western ECL Kit and competitor products
A

1000-100 ng BSA were separated in a 4-20% SDS-Page (FastGene® Precast Gel) using a primary antibody 1:5000 alpha-BSA (Invitrogen, A11133, corresponding  to 4 µg). All ECL kits were used under the same experimental workflow.

 

B

An IL-6 fusion protein was detected using a primary antibody against IL-6 from mouse and a secondary anti-mouse peroxide (POD) antibody 2 seconds exposure for the Western Blot. All ECL kits were used under the same experimental workflow.

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Downloads

Manual

Manual | FastGene Western ECL Kit | FG-CH1

MSDS

FG-CH01_MSDS

Technical Notes

Technical Note | FastGene Western ECL Kit | Detection limit for whole cell lysates

Technical Note | FastGene Western ECL Kit | Detection sensitivity comparison

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2 reviews for FastGene Western ECL Kit

  1. Daniel Hoff Institut für Pharmazie und Molekulare Biotechnologie Universität Heidelberg –

    Äußerst sensitiv bei einem deutlich besseren Preis verglichen mit ähnlichen Kits anderer Hersteller.
    Translated by NIPPON Genetics EUROPE
    Extremely sensitive, yet significantly better value for money compared to similar kits from other manufacturers.

    Verified User

  2. Katharina Gryksa-Zotz Lehrstuhl für Neurobiologie und Tierphysiologie Universität Regensburg –

    Wir sehen nach 30sec Inkubation bessere Protein Banden als nach 5min Inkubation mit einem vergleichbaren Kit eines anderen Herstellers.

    Translated by NIPPON Genetics EUROPE:
    We see better protein bands after 30 seconds of incubation than after 5 minutes of incubation with a comparable kit from another manufacturer.

    Verified User

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