FastGene® Western ECL Kit – Chemiluminescent Western Blot Detection
The FastGene® Western ECL Kit is an enhanced chemiluminescent (ECL) substrate based on luminol.
It is used to detect horseradish peroxidase (HRP) – conjugated secondary antibodies.
The high femtogram or low picogram detection of antigen is enabled by FastGene® Western ECL Kit brilliant sensitivity and long signal duration.
This long chemiluminescent signal duration makes it possible to detect signals on both digital and film-based imaging systems without any loss in signal intensity.
Appropriate primary and secondary antibody dilutions are suggested for attaining optimal signal intensity and duration.
Workflow using FastGene® Western ECL Kit
Mix the luminol solution and peroxide solution in a 1:1 ratio, and thoroughly agitate the chemiluminescent substrate solution well for preparing the 0.1 ml of solution / cm2 of membrane.
Place the membrane with the protein side up and remove the membrane from the chemiluminescent substrate solution. Image the membrane with a digital imager.