Biochemical protein analysis via blotting
Blotting of proteins is a powerful biochemical method for the detection of proteins. Protein bands that were separated by size after PAGE are transferred and immobilized on a carrier membrane. The firm binding of the proteins to the membrane allows a subsequent protein detection by choosing from a variety of staining or immunological methods.
Wet-transfer Western Blot system
The FastGene® Western Blot System is an efficient device to perform the first Western Blot protein transfer step from gel to membrane via a wet electropohoretic transfer technique. The Western Blot Systems contains all the necessary components for reliable protein transfer and comes with detailed guidelines to successfully perform transfer and Western Blot analysis.
The system includes a cool pack, which is installed during the run. The cool pack absobs generated running heat, avoiding the possibility of power loss. Gel and membrane can be easily sandwiched in the blot connector, which is assembled via an easy-to-use lock mechanism.
Below you find an image of the general Western Blot setup. For more detailed information, please have a look at the manual (click here).
Figure: General setup of the FastGene® Western Blot System.