PAGE stands for Polyacrylamide Gel Electrophoresis and describes an analytical method in biochemistry for the separation of differently sized protein mixtures in an electric field. During PAGE, proteins migrate through a gel matrix in response to an applied electic field. Smaller proteins travel faster through the gel than larger proteins, leading to a size dependent separation. The most popular form of PAGE is SDS-PAGE. The detergent sodium dodecyl sulfate (SDS) is added for PAGE sample preparation and is also part of the buffer composition. The treatment with the harsh detergent leads to full protein denaturation and unfolding. SDS binds to hydrophobic parts of the unfolded protein and masks the intrinsic charge of the protein with its own negative charge. As a consequence, SDS-protein complexes migration is predominantly dependent on the size of the protein, allowing an estimation of its molecular weight.
Complete set for PAGE protein separation
The FastGene® PAGE Protein System contains all the necessary equipment you need for PAGE protein analysis.
The electrophoresis tank can hold a maximum of 4 gels simultanously for electrophoretic protein separation.
Setup of the FastGene® PAGE Protein System
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Broad gel compatibility for highest flexibility
The FastGene® PAGE Protein System is compatible with a wide range of pre-cast gels (e.g., FastGene® gels, Bio-Rad Mini-PROTEAN® Precast Gels or ThermoFisher mini gels).
It also runs hand-cast gels included in the PAGE Protein System Set (for casting 1 mm thick gels). Also available are FastGene® hand-cast gel sets for gel preparation with 0.75 mm or 1.5 mm gel thickness (product link).
For use of different gels, three different U sealings are included in the FastGene® PAGE Protein System:
U sealing strip long, for 10 cm x 10 cm gels (e.g., ThermoFisher mini gels)
U sealing strip short, for 10 cm x 8 cm gels (Bio-Rad Mini-PROTEAN® Precast Gels and hand-cast gels)
U sealing strip FastGene®, for 10 cm x 8 cm gels (FastGene® gels)
Comfortable handling and setup
The FastGene® PAGE Protein System can easily be setup. Plastic dummy cassettes are included for flexible use with 1−4 gels.
Figure: General setup of the FastGene® PAGE Protein System (drawing).
Easiest gel hand-casting − No leaking!
The FastGene® PAGE Protein System includes a hand-cast set for casting 1 mm thick gels. The set alllows easiest gel hand-casting withour leaking!
Another gel-thickness is needed? No problem, 0.75 mm (PG02) and 1.5 mm (PG03) gel casting sets are separately available.
Figure: Gel hand-casting setup − Gel casting base with glass spacer, gel casting clips and sealing gasket.
Content and Accessories
The FastGene® PAGE Protein System (PG01) contains all the necessary eqipment for PAGE protein analysis and includes a hand-cast set for convenient gel casting.
Some content of PG01 is also separately available.
Content of FastGene® PAGE Protein System (PG01)
Component
Image
Description
Quantity inluded
Separately available as
Basic components
FastGene® Inner electrophoresis chamber with electrodes
The FastGene® PAGE Protein System also runs hand-cast gels included in the PAGE Protein System Set (for casting 1 mm thick gels).
Also available are FastGene® hand-cast gel sets for gel preparation with 0.75 mm or 1.5 mm gel thickness.
Bio-Rad™ Handcast Gels: The FastGene® PAGE Protein System is compatible with the Bio-Rad™Mini PROTEAN Tetra Handcast Gels. For more detailed information regarding the compatibility of the single components, please the section ‘Spare parts for Bio-Rad’.
Spare parts for Bio-Rad
Looking for spare parts for your Bio-Rad chamber?
The FastGene® PAGE Protein System is compatible with the Bio-Rad™Mini PROTEAN Tetra Handcast Gels 1.0 mm.
Do you have any questions about the product? Please contact us!
Rated 5 out of 5 based on 2 customer ratings
2 reviews for FastGene PAGE Protein System
Rated 5 out of 5
resnova top –
The innovative system from Nippon Genetics Europe, Gel Casting Clip, has allowed us to significantly speed up the preparation of Western Blot gels, as it does not allow any liquid leakage, so we no longer have to do the water leak test, it is small and handy. We already ordered 3 of them, with a particular Resnova promotion in March, and today we have ordered two more. I recommend anyone who makes homemade gels to use this system!
Rated 5 out of 5
resnova –
The innovative system from Nippon Genetics Europe, Gel Casting Clip, has allowed us to significantly speed up the preparation of Western Blot gels, as it does not allow any liquid leakage, so we no longer have to do the water leak test, it is small and handy. We already ordered 3 of them, with a particular Resnova promotion in March, and today we have ordered two more. I recommend anyone who makes homemade gels to use this system!
resnova top –
The innovative system from Nippon Genetics Europe, Gel Casting Clip, has allowed us to significantly speed up the preparation of Western Blot gels, as it does not allow any liquid leakage, so we no longer have to do the water leak test, it is small and handy. We already ordered 3 of them, with a particular Resnova promotion in March, and today we have ordered two more. I recommend anyone who makes homemade gels to use this system!
resnova –
The innovative system from Nippon Genetics Europe, Gel Casting Clip, has allowed us to significantly speed up the preparation of Western Blot gels, as it does not allow any liquid leakage, so we no longer have to do the water leak test, it is small and handy. We already ordered 3 of them, with a particular Resnova promotion in March, and today we have ordered two more. I recommend anyone who makes homemade gels to use this system!
Verified User