• FAS-Digi PRO gel documentation of DNA and RNA in agarose gels
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FastGene FAS-Digi PRO

Article number: GP-07LED

Price on request

Gel documentation system with innovative Blue/Green LED technology

 

Key Benefits

  • Documentation of nucleic acids in agarose gels
  • Light-sensitive camera with 24 MPixel and APS-C CMOS-sensor
  • Compatible with all common green, yellow and red DNA dyes
  • Camera studio software with comprehensive features for image acquisition
  • Fully networkable gel doc system: Easy transfer of images in JPEG and TIFF format when connected to a PC
  • Large illuminated imaging area for imaging multiple agarose gels of various size (26 x 21 cm)
  • Safe solution: No damage of DNA, no risk of UV exposure for users

The Revolution for Nucleic Acid Gel Documentation

The FastGene® FAS-Digi PRO is our newest imaging system for the detection of DNA and RNA in agarose gels. This gel doc system is a further development of our very successful FAS-Digi (GP-05LED). Equiped with a light-sensitive 24 MPixel camera, the FAS-Digi PRO is controlled completely by our newly developed imaging software. With the live view mode, all changes of the camera, the exposure time, the lens aperture, and a digital zoom are displayed in real-time. The FAS-Digi PRO is a fully networkable gel doc system, which allows a simple transfer of images when connected to a PC.

 

Blue/Green LED Light for a Safe Detection of DNA and RNA

The FastGene® FAS-Digi PRO is composed of a strong transilluminator equipped with the unique Blue/Green LED technology. These LEDs emit light at a wavelength of 470 nm – 520 nm without damaging nucleic acids (Figure 1). The Blue/Green LED light enables the detection of all common green dyes, such as MIDORIGreen or SYBRTM Green, yellow dyes e.g. SYBRTM Safe and red dyes, e.g. ethidium bromide or GelRedTM.

Absorbance Spectra of MIDORI Green and Ethidium bromide

Figure 1:  Absorbance spectra of Ethidium bromide (red), MIDORIGreen Advance (green) and MIDORIGreen Direct (light green). The Blue/Green LED light emit light at a wavelength of 470 – 520 nm, enabling the detection of all common green and red DNA dyes.

 

The imaging area of the transilluminator has a size of 26 x 21 cm, which allows the imaging of multiple agarose gels of various size. Because of the large illumination area and the possibility to remove the hood of the Fas-Digi PRO, it is very easy to excise DNA bands (Figure 2). The combination of our unique Blue/Green LED excitation technology with the high-end components deliver the best results with an easy to use gel doc system.

Figure 2: The hood of the FAS-Digi PRO can be easily removed. This allows a very easy excision of DNA bands.

Easy-To-Use Control Imaging Software

The FastGene® FAS-Digi PRO comes with the intuitive NIPPON Genetics Camera Studio software. With this software you can control all necessary parameters of the camera to analyse and optimize all your images: Just adjust the aperture, exposing time, sensitivity and focus and make images of the highest quality. A very nice feature is to control the focus by using a mouse wheel. Furthermore, you can select and enlarged the area of interest by using the mouse. Images can be recorded in the common TIFF and JPEG format. For prints just use a printer connected to your PC. Recommended printer is the thermal Mitsubishi Thermal Printer (P95DE), which creates brilliant prints on high-glossy paper.

 

Software of the FAS-Digi Pro Gel Doc system

Software of the FAS-Digi Pro Gel documentation system

Optimize and analyse your images with the NIPPON Genetics Camera Studio software. In real time you can control all settings of the camera, the aperture, exposure time, sensitivity. Very easy zoom function of an agarose gel, by dragging a frame of the area of interest with the computer mouse.

Camera for High Quality Agarose Gels

Take images of agarose gels of the highest quality using the Canon EOS 200D. This camera has an immense APS-C CMOS-sensor with a diameter of 22.3 x 14.9. The sensor produces no visible noise at all from ISO 100 all the way up to ISO 1600. Furthermore, the 24 megapixels allows the detection of lowest light signals in agarose gels.

The exposure time of the sensor can be set from 1/4000 sec up to 30 sec. A very short exposure guarantees that even the brightest DNA band will be visible within the dynamic range. Exposing the sensor for up 30 sec will enable the detection of even the faintest band. The 3x zoom (focal length of 18 mm to 55 mm) allows a perfect enlargement of the area of interest. Setting the lens at 18 mm allows the imaging of the complete illuminated area, while zooming into 55 mm will eliminate any unnecessary area.

The camera is directly connected to the power supply adapter of the Fas-Digi PRO. Therefore, the camera is constantly supplied with power.

Replacing batteries is no longer necessary.

 

FAS-Digi PRO high sensitive camera

The light sensitive camera of the FAS-Digi PRO has 24 megapixels and an APS-C CMOS sensor, allowing the analysis of agarose gels of the highest quality.

Camera

 

Camera typeCanon 200D, 24 MPixel (Resolution: 6000x4000), APS-C Sensor DIGIC 8 Processor, WiFi enabled
Image SensorAPS-C sized CMOS Sensor
Resolution/Image Size6000 x 4000 pixel
Bit Output Format8-bit
ApertureF/4-5.6
Lens18-55 mm zoom Lens, manual
Exposure time0.00025 to 30 seconds
FilterFastGene Amber Filter for the lens

 

Control Software

 

Control SoftwareNIPPON Genetics Camera Studio v1.0, WINDOWS 10
Saved Image FormatTIFF, JPEG
Image storageHost computer dependent
InterfaceHost computer dependent, Supports Mitsubishi thermal-printer P95DE

 

Hood

 

Power unitPower supply for transilluminator and camera
AccessFront door, 180°opened
FilterFastGene Amber Filter Shield

 

Illuminator

 

Built-in Blue-Green light source470-520 nm
View Area26 x 21 cm

 

Unit

MaterialPainted aluminum metal
Rated Voltage100-240 V~, 50/60 Hz, A
Dimension (H x L x W)52 x 33.5 x 32.5 cm
Weight14 kg

Is the thermoprinter Mitsubishi P95DE compatibe with the FAS-Digi PRO?

You can connect every printer directly or via network with the PC, which controlls the FAS-Digi PRO. If you want to connect a printer directly to the camera, without using a PC. we recommen the Canon Selphy, a Wifi photo-printer.

on 09/10/2019

Wir haben das FastGene FAS-Digi PRO seit einiger Zeit in Gebrauch und sind sowohl mit der benutzerfreundlichen Handhabung der Software, wie auch der Qualität der Aufnahmen sehr zufrieden. Wir verwenden als Farbstoff Ethidiumbromid, womit DNA im Gel sehr gut detektierbar wird. Von uns wurden keine nennenswerten Unterschiede zur Detektion mit herkömmlichen UV-Transilluminatoren festgestellt. Lediglich das Ausschneiden von Banden aus einem Gel gefällt uns an dem System nicht so gut. Die vom Hersteller vorgesehen Entfernung der Dunkelkammer empfinden wir als etwas unhandlich. Wir haben uns zu diesem Zweck einen selbstgebauten Amber-Filter oben an der Kammer befestigt, der jetzt bei Bedarf heruntergeklappt werden kann. Mit dieser Modifikation gefällt uns das Geldokumentationssystem sehr gut und können es mit gutem Gewissen weiterempfehlen.

Translation by NIPPON Genetics EUROPE:

We have been using the FastGene FAS-Digi PRO for some time now and are very satisfied with the user-friendly handling of the software and the quality of the recordings. We use ethidium bromide as a dye, with which the DNA detection is very good. We did not find any significant difference in signal to the detection using conventional UV transilluminators. Only the cutting out of bands from a gel we like the system could be optimized. The removal of the darkroom provided by the manufacturer is perceived as being somewhat unwieldy. For this purpose, we have attached a self-made amber filter to the top of the chamber, which can now be folded down if necessary. With this modification, we like the gel documentation system very well and can recommend without hesitation.

Comment from NIPPON Genetics EUROPE:

Thank you very much for your honest feedback. We will use your recommendation to optimize our FAS Digi PRO. We will contact you shortly.

on 09/09/2019

Wir arbeiten nun seit einigen Monaten zu unserer großen Zufriedenheit mit dem FastGene FAS-Digi Pro in Kombination mit dem Farbstoff Midori Green. DNA Proben sind sehr gut detektierbar und dank der mitgelieferten Software sind leicht und schnell sehr gute Bilder gemacht. Einziges Manko: Zum Ausschneiden von Gelbanden, wäre ein beiliegendes Plexiglas, das durch eine Erhöhung einer Seite schief in der Kammer liegt von Vorteil, da man so in einem 90° Winkel auf das Gel gucken kann und somit genauere Schnitte durchführen werden könnten. Das Geldokumentationssystem erfüllt bis auf diesen Punkt alle unsere Erwartung. Wir würden das FastGene FAS-DIGI jederzeit weiterempfehlen.

Translation by NIPPON Genetics EUROPE:

We have been working for a few months with the FastGene FAS-Digi Pro with great satisfaction using the dye Midori Green. DNA samples are very easy to detect and thanks to the supplied software, very good pictures are taken quickly and easily. The only drawback: To cut out gel bands, an enclosed Filter, which is attached to the system´s chamber could be advantageous because you can look at a 90 ° angle to the gel and thus more accurate cuts could be performed. The gel documentation system meets all our expectations to this point. We would recommend the FastGene FAS-DIGI Pro at any time.

Comment from NIPPON Genetics EUROPE:

Thank you very much for your honest feedback. We will follow your recommendation and integrate an amber filter to the system to facilitate the cutting of the bands
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