The FastGene® FAS-Digi PRO is our newest imaging system for the detection of DNA and RNA in agarose gels. This gel doc system is a further development of our very successful FAS-Digi (GP-05LED). Equiped with a light-sensitive 24 MPixel camera, the FAS-Digi PRO is controlled completely by our newly developed imaging software. With the live view mode, all changes of the camera, the exposure time, the lens aperture, and a digital zoom are displayed in real-time. The FAS-Digi PRO is a fully networkable gel doc system, which allows a simple transfer of images when connected to a PC.
The FastGene® FAS-Digi PRO is composed of a strong transilluminator equipped with the unique Blue/Green LED technology. These LEDs emit light at a wavelength of 470 nm – 520 nm without damaging nucleic acids (Figure 1). The Blue/Green LED light enables the detection of all common green dyes, such as MIDORIGreen or SYBRTM Green, yellow dyes e.g. SYBRTM Safe and red dyes, e.g. ethidium bromide or GelRedTM.
Figure 1: Absorbance spectra of Ethidium bromide (red), MIDORIGreen Advance (green) and MIDORIGreen Direct (light green). The Blue/Green LED light emit light at a wavelength of 470 – 520 nm, enabling the detection of all common green and red DNA dyes.
The imaging area of the transilluminator has a size of 26 x 21 cm, which allows the imaging of multiple agarose gels of various size. Because of the large illumination area and the possibility to remove the hood of the Fas-Digi PRO, it is very easy to excise DNA bands (Figure 2). The combination of our unique Blue/Green LED excitation technology with the high-end components deliver the best results with an easy to use gel doc system.
Figure 2: The hood of the FAS-Digi PRO can be easily removed. This allows a very easy excision of DNA bands.
The FastGene® FAS-Digi PRO comes with the intuitive NIPPON Genetics Camera Studio software. With this software you can control all necessary parameters of the camera to analyse and optimize all your images: Just adjust the aperture, exposing time, sensitivity and focus and make images of the highest quality. A very nice feature is to control the focus by using a mouse wheel. Furthermore, you can select and enlarged the area of interest by using the mouse. Images can be recorded in the common TIFF and JPEG format. For prints just use a printer connected to your PC. Recommended printer is the thermal Mitsubishi Thermal Printer (P95DE), which creates brilliant prints on high-glossy paper.
Optimize and analyse your images with the NIPPON Genetics Camera Studio software. In real time you can control all settings of the camera, the aperture, exposure time, sensitivity. Very easy zoom function of an agarose gel, by dragging a frame of the area of interest with the computer mouse.
Take images of agarose gels of the highest quality using the Canon EOS 200D. This camera has an immense APS-C CMOS-sensor with a diameter of 22.3 x 14.9. The sensor produces no visible noise at all from ISO 100 all the way up to ISO 1600. Furthermore, the 24 megapixels allows the detection of lowest light signals in agarose gels.
The exposure time of the sensor can be set from 1/4000 sec up to 30 sec. A very short exposure guarantees that even the brightest DNA band will be visible within the dynamic range. Exposing the sensor for up 30 sec will enable the detection of even the faintest band. The 3x zoom (focal length of 18 mm to 55 mm) allows a perfect enlargement of the area of interest. Setting the lens at 18 mm allows the imaging of the complete illuminated area, while zooming into 55 mm will eliminate any unnecessary area.
The camera is directly connected to the power supply adapter of the Fas-Digi PRO. Therefore, the camera is constantly supplied with power.
Replacing batteries is no longer necessary.
The light sensitive camera of the FAS-Digi PRO has 24 megapixels and an APS-C CMOS sensor, allowing the analysis of agarose gels of the highest quality.
|Camera type||Canon 200D, 24 MPixel (Resolution: 6000x4000), APS-C Sensor DIGIC 8 Processor, WiFi enabled|
|Image Sensor||APS-C sized CMOS Sensor|
|Resolution/Image Size||6000 x 4000 pixel|
|Bit Output Format||8-bit|
|Lens||18-55 mm zoom Lens, manual|
|Exposure time||0.00025 to 30 seconds|
|Filter||MIDORI Green Amber Filter|
|Control Software||NIPPON Genetics Camera Studio v1.0, WINDOWS 10|
|Saved Image Format||TIFF, JPEG|
|Image storage||Host computer dependent|
|Interface||Host computer dependent, Supports Mitsubishi thermal-printer P95DE|
|Access||Front door, 180°opened|
|Power unit||Power supply for transilluminator and camera|
|Built-in Blue-Green light source||470-520 nm|
|View Area||26 x 21 cm|
|Material||Painted aluminum metal|
|Rated Voltage||100-240 V~, 50/60 Hz, A|
|Dimension (H x L x W)||52 x 33.5 x 32.5 cm|