Kickspeed 2x DNA Ligation Mix
The FastGene® Kickspeed 2X DNA Ligation Mix is a ready-to-use solution of T4 DNA Ligase for both sticky-end and blunt-end ligation reactions. This master mix enables quick ligation in a short incubation time (< 5min) at room temperature.
- 2X Master mix of T4 DNA Ligase and ligation enhancer
- Fastest ligation of dsDNA with sticky end and blunt end
- Incubation-free ligation
- Vector construction
- Linker ligation
- Fragment assembly
- Routine cloning
T4 DNA Ligase
The FastGene® T4 DNA Ligase catalyzes the formation of a covalent bond between the 5′-phosphate and 3′-OH in nicked duplex DNA or at two DNA ends. This activity is very useful to ligate DNA fragments with either cohesive or blunt ends, that are generated by restriction enzyme digestion. T4 DNA Ligase can also ligate RNA with DNA or RNA in a double helix with low efficiency. The T4 DNA Ligase is cloned and expressed in E. coli, and purified to homogeneity. The Ligase is free of endonuclease, exonuclease and phosphatase.