Hae II

Restriction Enzyme

Recognition Sequence	Amount
RGCGC/Y Y/CGCGR	2000 Units, 20 Units/µL

Overhang	CpG Methylation
3′ GCGC	Sensitive

Incubation Temp.	Heat Inactivation
37°C	80°C for 20 min

Activity in FastGene Buffer

Buffer I	Buffer II	Buffer III	Buffer IV	FastCut Buffer
10%	100%	100%	100%	100%

Supplied Buffer	Enzyme Type
Buffer IV	Type IIP

Isoschizomers
BfoI, BstH2I

Cat. No. FG-HaeII

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