Not I

Restriction Enzyme

Recognition Sequence	Amount
GC/GGCCGC CGCCGG/CG	500 Units, 10 Units/µL

Overhang	CpG Methylation
5′ GGCC	Sensitive

Incubation Temp.	Heat Inactivation
37°C	65°C for 20 min

Activity in FastGene Buffer

Buffer I	Buffer II	Buffer III	Buffer IV	FastCut Buffer
0%	50%	100%	0%	100%

Supplied Buffer	Enzyme Type
Buffer III	Type IIP

Isoschizomer
CciNI

Cat. No. FG-NotI

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