Ple I

Restriction Enzyme

Recognition Sequence	Amount
GAGTCNNNN/N CTCAGNNNNN/	1000 Units, 5 Units/µL

Overhang	CpG Methylation
5′ N	Conditionally sensitive

Incubation Temp.	Heat Inactivation
37°C	65°C for 20 min

Activity in FastGene Buffer

Buffer I	Buffer II	Buffer III	Buffer IV	FastCut Buffer
75%	75%	50%	100%	100%

Supplied Buffer	Enzyme Type
Buffer IV	Type IIS

Isoschizomers
MlyI, PpsI, SchI

Cat. No. FG-PleI

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