MIDORIGreen Advance TBE Agarose Tablets – safe, clean and fast
The MIDORIGreen Agarose Tablets are a clean and fast solution for preparing agarose gels without time-consuming weighing. The agarose tablet only has to be solved in pure water. After heating and pouring of the solution the gel is ready for loading. As MIDORIGreen DNA Stain, TBE powder and agarose are integrated components, there is no addition of other ingredients necessary. In particular for researchers, who are preparing many agarose gels, this is a very comfortable and easy solution to save time. Another big advantage is the safety for the user. As a safe alternative to ethidium bromide MIDORIGreen Advance is used as DNA stain. MIDORIGreen Advance DNA Stain is non-carcinogenic – hence it is optimal for a safe work with agarose gels and no special disposing of waste is necessary.
||MIDORI Green amount (µL)
||MIDORI Green calculated amount in 100 mL gel*
|1.0 % gel
|1.2 % gel
|1.5 % gel
|2.0 % gel
*TBE is suitable to use in rage 0.5 – 1 x concentration.
**MIDORIGreen DNA stain optimum is 4-6 µL per 100 mL agarose gel.
If I have to use 50ml of water to dissolve 1 tablet of AG09 to generate 1% agarose gel, will I be able to produce 1.5% or 2% agarose solution by varying the quantity of water?
Yes, it is exactly as you presumed. You can reduce the amount of water to the half to get a 2% agarose solution. With 2% agarose we get satisfying results. I wouldn’t recommend to increase the percentage much more than 2%, because otherwise the background could be to strong (theoretically).
What temperature (approximately) is needed to completely dissolve the tablet using microwave oven? Can the tablet be dissolved first and stored? If yes, how long can the gel be stored?
In the beginning you solve the tablet in cold distilled water (water with room temperature is fine) till it is completely solved. From experience it takes around one minute. It is easy to observe the solving. After the disintegration of the tablet you heat the mixture till you can’t see any streaks. For the heating you have different options. The easiest it to choose the microwave. You will heat the mixture as long as it is boiling (around 30 sec in the microwave) to be sure to solve the agarose completely. I suppose you mean if the casted gel could be stored? Yes, that is possible but you have to ensure that the gel is wrapped in a wet tissue (and additionally with a foil so that it can’t dry out) and isn’t affected by light. You can store the gels for one week like that.
Can MG Agarose Tablets bs used with TAE buffer?
We wouldn’t recommend to use TAE buffer. The gel is a TBE gel and using TAE running buffer wouldn’t result in an optimal running behavior because of different conductivity.
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Japanese Researcher, Department of Health Sciences, Faculty of Medicine, University of the Ryukyus –
No weighing is necessary and it is very convenient to use. It’s easy to melt, and detection of bands is pretty clear.
Kyushu Institute of Technology, Research Bioinformatics Research Inst. Yasunaga Laboratory, Y. M. –
Easy to handle. The procedure is very fast.
Japanese Researcher at Kinjo Gakuin University –
EtBr is unnecessary, results can be obtained immediately. Easy to use because it is a tablet.
Japanese Researcher, RIKEN –
We can create a gel without weighing agarose. I made a 4% gel, without any problems.
Eiji Hisanaga, Biological Department of Science, Tokyo Igusa High School –
Very easy to handle. No worry about about toxicity such as Ethidium Bromide. A agarose gel can be made easily without concentration calculation.
Research Department, Research Department, Nissui Pharmaceutical Co., Ltd, J.T. –
Extremely good for safety and disposal. Not neccessary to use carcinogenic DNA dyes such as Ethidium bromide
Mr. Isao Abe, Miyagi prefecture Furukawa Industrial High School Department of Chemical Technology –
Easy to prepare a agarose gel with the target concentration and the nucleic acid fluorescent reagent. It is unnecessary to perform other operations than melting and heating.
Nagasaki University Graduate School of Medicine and Dentistry Graduate School of Medicine and Dentistry Genomic Clinical Pharmacy Laboratory, N.B. –
Very easy to use
St. Marianna University School of Medicine School of Obstetrics and Gynecology, Atsushi Uekawa –
Very easy gel preparation without weighing agarose and addition of the DNA dye.
Department of Health Sciences, Faculty of Medicine, University of the Ryukyus Department of Biological Metabolism, HR –
Easy to use without weighing the agarose. Furthermore the agarose is easy to melt and the band detection is also beautiful.